Organs and Mini-Organs combines contributions from leading practitioners who work under the editorial control of an acclaimed researcher who also served for eight years as Editor-in-Chief of the journal Organogenesis, the first journal on this topic. The book begins with an introduction, but then delves into chapters that present advice on how to make organoids for many systems. In addition, case studies that illustrate the uses of organioids are presented, along with discussions on future directions and specific problems that need to be solved.
1. Part I: Introduction - p. xviii 2. Chapter 1. Organoids and mini-organs: Introduction, history, and potential - p. 3 3. Part II: Constructing Organoids - p. 18 4. Chapter 2. Elements of organoid design - p. 27 5. Chapter 3. Intestinal organoids: Mini-guts grown in the laboratory - p. 43 6. Chapter 4. Three-dimensional breast culture models: New culture models for analyzing breast development and function - p. 73 7. Chapter 5. Prostate organoids: Directed differentiation from embryonic stem cells - p. 89 8. Chapter 6. Kidney organoids - p. 117 9. Chapter 7. Spontaneous self-assembly of liver organoids from differentiated human cells: Human liver organoids - p. 145 10. Chapter 8. Cerebral organoids: Building brains from stem cells - p. 157 11. Chapter 9. From organoids to mini-organs: A case study in the kidney - p. 175 12. Part III: Applications of Organoids - p. 189 13. Chapter 10. Intestinal organoids: A model for biomedical and nutritional research - p. 195 14. Chapter 11. A three-dimensional neurosphere system using human stem cells for nanotoxicology studies - p. 215 15. Chapter 12. Organoids for modeling kidney disease - p. 227 16. Chapter 13. Intestinal organoids in studying host−bacterial interactions - p. 247 17. Chapter 14. Four challenges for organoid engineers - p. 255 18. Index - p. 261
Organs and Mini-Organs combines contributions from leading practitioners who work under the editorial control of an acclaimed researcher who also served for eight years as Editor-in-Chief of the journal Organogenesis, the first journal on this topic. The book begins with an introduction, but then delves into chapters that present advice on how to make organoids for many systems. In addition, case studies that illustrate the uses of organioids are presented, along with discussions on future directions and specific problems that need to be solved.
1. Part I: Introduction - p. xviii 2. Chapter 1. Organoids and mini-organs: Introduction, history, and potential - p. 3 3. Part II: Constructing Organoids - p. 18 4. Chapter 2. Elements of organoid design - p. 27 5. Chapter 3. Intestinal organoids: Mini-guts grown in the laboratory - p. 43 6. Chapter 4. Three-dimensional breast culture models: New culture models for analyzing breast development and function - p. 73 7. Chapter 5. Prostate organoids: Directed differentiation from embryonic stem cells - p. 89 8. Chapter 6. Kidney organoids - p. 117 9. Chapter 7. Spontaneous self-assembly of liver organoids from differentiated human cells: Human liver organoids - p. 145 10. Chapter 8. Cerebral organoids: Building brains from stem cells - p. 157 11. Chapter 9. From organoids to mini-organs: A case study in the kidney - p. 175 12. Part III: Applications of Organoids - p. 189 13. Chapter 10. Intestinal organoids: A model for biomedical and nutritional research - p. 195 14. Chapter 11. A three-dimensional neurosphere system using human stem cells for nanotoxicology studies - p. 215 15. Chapter 12. Organoids for modeling kidney disease - p. 227 16. Chapter 13. Intestinal organoids in studying host−bacterial interactions - p. 247 17. Chapter 14. Four challenges for organoid engineers - p. 255 18. Index - p. 261
