Patient Derived Tumor Xenograft Models: Promise, Potential and Practice offers guidance on how to conduct PDX modeling and trials, including how to know when these models are appropriate for use, and how the data should be interpreted through the selection of immunodeficient strains.
In addition, proper methodologies suitable for growing different type of tumors, acquisition of pathology, genomic and other data about the tumor, potential pitfalls, and confounding background pathologies that occur in these models are also included, as is a discussion of the facilities and infrastructure required to operate a PDX laboratory.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. List of Contributors - p. xvii 6. Biographies - p. xxi 7. Foreword - p. xxiii 8. Preface - p. xxv 9. Section I. Mouse Xenograft Models of Cancer - p. xxvi 10. Chapter 1. PDX Models: History and Development - p. 1 11. Chapter 2. History of Mouse Cancer Models - p. 15 12. Chapter 3. Challenges and Limitations of Mouse Xenograft Models of Cancer - p. 25 13. Chapter 4. Tumor Heterogeneity - p. 37 14. Chapter 5. Immunodeficient Mice: The Backbone of Patient-Derived Tumor Xenograft Models - p. 57 15. Chapter 6. Humanized Mice and PDX Models - p. 75 16. Section II. Components of a PDX Program - p. 89 17. Chapter 1. Regulations of Patient-Derived Xenografts - p. 93 18. Chapter 2. Acquisition and Storage of Clinical Samples to Establish PDX Models - p. 109 19. Chapter 3. Methodologies for Developing and Maintaining Patient-Derived Xenograft Mouse Models - p. 119 20. Chapter 4. Pathology of Patient-Derived Xenograft Tumors - p. 135 21. Chapter 5. Genetic Profiling of Tumors in PDX Models - p. 149 22. Chapter 6. Running a PDX Core Laboratory or a PDX Support Program - p. 161 23. Chapter 7. Veterinary Care - p. 173 24. Chapter 8. Occupational Health and Safety - p. 187 25. Section III. PDX Models for Tumors of Various Organ Systems - p. 202 26. Chapter 1. Pediatric and Adult Brain Tumor PDX Models - p. 205 27. Chapter 2. Patient-Derived Xenograft Models of Prostate Tumors - p. 217 28. Chapter 3. Patient-Derived Xenograft Model of Pancreatic Cancer - p. 229 29. Chapter 4. Modeling Breast Cancer Heterogeneity With Patient-Derived Xenografts - p. 243 30. Chapter 5. Patient-Derived Xenograft Models of Ovarian/Gynecologic Tumors - p. 257 31. Chapter 6. Patient-Derived Xenografts From Lung Cancer and Their Potential Applications - p. 273 32. Chapter 7. PDX Models of Colorectal Tumors - p. 291 33. Chapter 8. Patient-Derived Tumor Xenografts in Hematologic Disorders - p. 305 34. Chapter 9. Patient-Derived Xenografting of Human Melanoma - p. 341 35. Chapter 10. Advances in Organoid Culturing of Patient-Derived Tumors - p. 365 36. Section IV. PDX Models in Cancer Research and Therapy Around the World - p. 375 37. Chapter 1. Global Practices in PDX Programs - p. 379 38. Chapter 2. Role of Companies and Corporations in the Development and Utilization of PDX Models - p. 409 39. Section V. Challenges & Future of PDX Models - p. 426 40. Chapter 1. Patient-Derived Tumor Xenograft: Present and Future Challenges and Applications - p. 429 41. Index - p. 453
Patient Derived Tumor Xenograft Models: Promise, Potential and Practice offers guidance on how to conduct PDX modeling and trials, including how to know when these models are appropriate for use, and how the data should be interpreted through the selection of immunodeficient strains.
In addition, proper methodologies suitable for growing different type of tumors, acquisition of pathology, genomic and other data about the tumor, potential pitfalls, and confounding background pathologies that occur in these models are also included, as is a discussion of the facilities and infrastructure required to operate a PDX laboratory.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. List of Contributors - p. xvii 6. Biographies - p. xxi 7. Foreword - p. xxiii 8. Preface - p. xxv 9. Section I. Mouse Xenograft Models of Cancer - p. xxvi 10. Chapter 1. PDX Models: History and Development - p. 1 11. Chapter 2. History of Mouse Cancer Models - p. 15 12. Chapter 3. Challenges and Limitations of Mouse Xenograft Models of Cancer - p. 25 13. Chapter 4. Tumor Heterogeneity - p. 37 14. Chapter 5. Immunodeficient Mice: The Backbone of Patient-Derived Tumor Xenograft Models - p. 57 15. Chapter 6. Humanized Mice and PDX Models - p. 75 16. Section II. Components of a PDX Program - p. 89 17. Chapter 1. Regulations of Patient-Derived Xenografts - p. 93 18. Chapter 2. Acquisition and Storage of Clinical Samples to Establish PDX Models - p. 109 19. Chapter 3. Methodologies for Developing and Maintaining Patient-Derived Xenograft Mouse Models - p. 119 20. Chapter 4. Pathology of Patient-Derived Xenograft Tumors - p. 135 21. Chapter 5. Genetic Profiling of Tumors in PDX Models - p. 149 22. Chapter 6. Running a PDX Core Laboratory or a PDX Support Program - p. 161 23. Chapter 7. Veterinary Care - p. 173 24. Chapter 8. Occupational Health and Safety - p. 187 25. Section III. PDX Models for Tumors of Various Organ Systems - p. 202 26. Chapter 1. Pediatric and Adult Brain Tumor PDX Models - p. 205 27. Chapter 2. Patient-Derived Xenograft Models of Prostate Tumors - p. 217 28. Chapter 3. Patient-Derived Xenograft Model of Pancreatic Cancer - p. 229 29. Chapter 4. Modeling Breast Cancer Heterogeneity With Patient-Derived Xenografts - p. 243 30. Chapter 5. Patient-Derived Xenograft Models of Ovarian/Gynecologic Tumors - p. 257 31. Chapter 6. Patient-Derived Xenografts From Lung Cancer and Their Potential Applications - p. 273 32. Chapter 7. PDX Models of Colorectal Tumors - p. 291 33. Chapter 8. Patient-Derived Tumor Xenografts in Hematologic Disorders - p. 305 34. Chapter 9. Patient-Derived Xenografting of Human Melanoma - p. 341 35. Chapter 10. Advances in Organoid Culturing of Patient-Derived Tumors - p. 365 36. Section IV. PDX Models in Cancer Research and Therapy Around the World - p. 375 37. Chapter 1. Global Practices in PDX Programs - p. 379 38. Chapter 2. Role of Companies and Corporations in the Development and Utilization of PDX Models - p. 409 39. Section V. Challenges & Future of PDX Models - p. 426 40. Chapter 1. Patient-Derived Tumor Xenograft: Present and Future Challenges and Applications - p. 429 41. Index - p. 453
