De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface - p. xix 7. Chapter One: Chemical Posttranslational Modification with Designed Rhodium(II) Catalysts - p. xxii 8. Chapter Two: Cell-Binding Assays for Determining the Affinity of Protein–Protein Interactions: Technologies and Considerations - p. 19 9. Chapter Three: Protein and Antibody Engineering by Phage Display - p. 44 10. Chapter Four: Incorporation of Unnatural Amino Acids into Proteins Expressed in Mammalian Cells - p. 87 11. Chapter Five: Method for Enzyme Design with Genetically Encoded Unnatural Amino Acids - p. 107 12. Chapter Six: Methods for Solving Highly Symmetric De Novo Designed Metalloproteins: Crystallographic Examination of a Novel Three-Stranded Coiled-Coil Structure Containing d-Amino Acids - p. 133 13. Chapter Seven: SpyRings Declassified: A Blueprint for Using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal Resilience - p. 148 14. Chapter Eight: Engineering and Application of LOV2-Based Photoswitches - p. 167 15. Chapter Nine: Minimalist Design of Allosterically Regulated Protein Catalysts - p. 190 16. Chapter Ten: Combining Design and Selection to Create Novel Protein–Peptide Interactions - p. 202 17. Chapter Eleven: Metal-Directed Design of Supramolecular Protein Assemblies - p. 222 18. Chapter Twelve: Designing Fluorinated Proteins - p. 250 19. Chapter Thirteen: Solid Phase Synthesis of Helically Folded Aromatic Oligoamides - p. 278 20. Chapter Fourteen: Conformational Restriction of Peptides Using Dithiol Bis-Alkylation - p. 301 21. Chapter Fifteen: Engineering Short Preorganized Peptide Sequences for Metal Ion Coordination: Copper(II) a Case Study - p. 332 22. Chapter Sixteen: De Novo Construction of Redox Active Proteins - p. 364 23. Chapter Seventeen: Design Strategies for Redox Active Metalloenzymes: Applications in Hydrogen Production - p. 388 24. Chapter Eighteen: Equilibrium Studies of Designed Metalloproteins - p. 416 25. Chapter Nineteen: Reconstitution of Heme Enzymes with Artificial Metalloporphyrinoids - p. 438 26. Chapter Twenty: Creation of a Thermally Tolerant Peroxidase - p. 454 27. Chapter Twenty-One: Designing Covalently Linked Heterodimeric Four-Helix Bundles - p. 470 28. Chapter Twenty-Two: Design of Heteronuclear Metalloenzymes - p. 499 29. Chapter Twenty-Three: Periplasmic Screening for Artificial Metalloenzymes - p. 537 30. Chapter Twenty-Four: De Novo Designed Imaging Agents Based on Lanthanide Peptides Complexes - p. 556 31. Chapter Twenty-Five: Peptide Binding for Bio-Based Nanomaterials - p. 580 32. Author Index - p. 599 33. Subject Index - p. 639
De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface - p. xix 7. Chapter One: Chemical Posttranslational Modification with Designed Rhodium(II) Catalysts - p. xxii 8. Chapter Two: Cell-Binding Assays for Determining the Affinity of Protein–Protein Interactions: Technologies and Considerations - p. 19 9. Chapter Three: Protein and Antibody Engineering by Phage Display - p. 44 10. Chapter Four: Incorporation of Unnatural Amino Acids into Proteins Expressed in Mammalian Cells - p. 87 11. Chapter Five: Method for Enzyme Design with Genetically Encoded Unnatural Amino Acids - p. 107 12. Chapter Six: Methods for Solving Highly Symmetric De Novo Designed Metalloproteins: Crystallographic Examination of a Novel Three-Stranded Coiled-Coil Structure Containing d-Amino Acids - p. 133 13. Chapter Seven: SpyRings Declassified: A Blueprint for Using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal Resilience - p. 148 14. Chapter Eight: Engineering and Application of LOV2-Based Photoswitches - p. 167 15. Chapter Nine: Minimalist Design of Allosterically Regulated Protein Catalysts - p. 190 16. Chapter Ten: Combining Design and Selection to Create Novel Protein–Peptide Interactions - p. 202 17. Chapter Eleven: Metal-Directed Design of Supramolecular Protein Assemblies - p. 222 18. Chapter Twelve: Designing Fluorinated Proteins - p. 250 19. Chapter Thirteen: Solid Phase Synthesis of Helically Folded Aromatic Oligoamides - p. 278 20. Chapter Fourteen: Conformational Restriction of Peptides Using Dithiol Bis-Alkylation - p. 301 21. Chapter Fifteen: Engineering Short Preorganized Peptide Sequences for Metal Ion Coordination: Copper(II) a Case Study - p. 332 22. Chapter Sixteen: De Novo Construction of Redox Active Proteins - p. 364 23. Chapter Seventeen: Design Strategies for Redox Active Metalloenzymes: Applications in Hydrogen Production - p. 388 24. Chapter Eighteen: Equilibrium Studies of Designed Metalloproteins - p. 416 25. Chapter Nineteen: Reconstitution of Heme Enzymes with Artificial Metalloporphyrinoids - p. 438 26. Chapter Twenty: Creation of a Thermally Tolerant Peroxidase - p. 454 27. Chapter Twenty-One: Designing Covalently Linked Heterodimeric Four-Helix Bundles - p. 470 28. Chapter Twenty-Two: Design of Heteronuclear Metalloenzymes - p. 499 29. Chapter Twenty-Three: Periplasmic Screening for Artificial Metalloenzymes - p. 537 30. Chapter Twenty-Four: De Novo Designed Imaging Agents Based on Lanthanide Peptides Complexes - p. 556 31. Chapter Twenty-Five: Peptide Binding for Bio-Based Nanomaterials - p. 580 32. Author Index - p. 599 33. Subject Index - p. 639
