Methods in Enzymology, Volume 607: Phosphatases, the latest release in this ongoing series, highlights new advances in the field as detailed by an international board of authors. This latest release includes chapters on Empirical Valence Bond Simulations of the Evolution of Enzyme Function, QM/MM Free Energy and Kinetic Isotope Analysis of Phosphoryl Transfer in Enzymes, the Structural, Mechanism and Evolution of Phosphatases, How to Define Rapid Motions in Pumping Pyrophosphatases, The Evolution of K+-Independence in Pyrophosphatases, the Crystallization of Michaelis, Intermediate and Inhibited Complexes in Phosphatases, and an Investigation of Nucleotide Loading and Effector Binding of K-Ras.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Section I: Computational Analysis of Enzymatic Phosphoryl Transfer - p. xviii 8. Chapter One: Empirical Valence Bond Simulations of Organophosphate Hydrolysis: Theory and Practice - p. xviii 9. Chapter Two: Analysis of Phosphoryl-Transfer Enzymes with QM/MM Free Energy Simulations - p. 51 10. Section II: Structure, Mechanism and Evolution of Phosphatases - p. 90 11. Chapter Three: Defining Dynamics of Membrane-Bound Pyrophosphatases by Experimental and Computational Single-Molecule FRET - p. 90 12. Chapter Four: A Simple Strategy to Determine the Dependence of Membrane-Bound Pyrophosphatases on K+ as a Cofactor - p. 130 13. Chapter Five: Crystallization of Liganded Phosphatases in the HAD Superfamily - p. 156 14. Section III: Substrate Specificity and Enzyme Conformational States - p. 184 15. Chapter Six: Structure, Mechanism, and Substrate Profiles of the Trinuclear Metallophosphatases from the Amidohydrolase Superfamily - p. 184 16. Chapter Seven: Affinities and Comparisons of Enzyme States by Principal Component Analysis of NMR Spectra, Automated using TREND Software - p. 216 17. Chapter Eight: Assessment and Impacts of Phosphorylation on Protein Flexibility of the α-d-Phosphohexomutases - p. 240 18. Chapter Nine: Chemical Tools for Studying the Impact of cis/trans Prolyl Isomerization on Signaling: A Case Study on RNA Polymerase II Phosphatase Activity and Specificity - p. 267 19. Section IV: Delineating the Action of Phosphatases in Signaling and Regulation - p. 297 20. Chapter Ten: Quantitative Analysis of Intracellular Response Regulator Phosphatase Activity of Histidine Kinases - p. 297 21. Chapter Eleven: Measuring the Activities of Two-Component Regulatory System Phosphatases - p. 319 22. Chapter Twelve: Assembly of PGAM5 into Multimeric Complexes Provides a Mechanism for Allosteric Regulation of Phosphatase Activity - p. 351 23. Chapter Thirteen: Purification of Lipin and Measurement of Phosphatidic Acid Phosphatase Activity from Liposomes - p. 372 24. Chapter Fourteen: Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities - p. 388 25. Chapter Fifteen: Protein Chemical Approaches to Understanding PTEN Lipid Phosphatase Regulation - p. 403
Methods in Enzymology, Volume 607: Phosphatases, the latest release in this ongoing series, highlights new advances in the field as detailed by an international board of authors. This latest release includes chapters on Empirical Valence Bond Simulations of the Evolution of Enzyme Function, QM/MM Free Energy and Kinetic Isotope Analysis of Phosphoryl Transfer in Enzymes, the Structural, Mechanism and Evolution of Phosphatases, How to Define Rapid Motions in Pumping Pyrophosphatases, The Evolution of K+-Independence in Pyrophosphatases, the Crystallization of Michaelis, Intermediate and Inhibited Complexes in Phosphatases, and an Investigation of Nucleotide Loading and Effector Binding of K-Ras.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Section I: Computational Analysis of Enzymatic Phosphoryl Transfer - p. xviii 8. Chapter One: Empirical Valence Bond Simulations of Organophosphate Hydrolysis: Theory and Practice - p. xviii 9. Chapter Two: Analysis of Phosphoryl-Transfer Enzymes with QM/MM Free Energy Simulations - p. 51 10. Section II: Structure, Mechanism and Evolution of Phosphatases - p. 90 11. Chapter Three: Defining Dynamics of Membrane-Bound Pyrophosphatases by Experimental and Computational Single-Molecule FRET - p. 90 12. Chapter Four: A Simple Strategy to Determine the Dependence of Membrane-Bound Pyrophosphatases on K+ as a Cofactor - p. 130 13. Chapter Five: Crystallization of Liganded Phosphatases in the HAD Superfamily - p. 156 14. Section III: Substrate Specificity and Enzyme Conformational States - p. 184 15. Chapter Six: Structure, Mechanism, and Substrate Profiles of the Trinuclear Metallophosphatases from the Amidohydrolase Superfamily - p. 184 16. Chapter Seven: Affinities and Comparisons of Enzyme States by Principal Component Analysis of NMR Spectra, Automated using TREND Software - p. 216 17. Chapter Eight: Assessment and Impacts of Phosphorylation on Protein Flexibility of the α-d-Phosphohexomutases - p. 240 18. Chapter Nine: Chemical Tools for Studying the Impact of cis/trans Prolyl Isomerization on Signaling: A Case Study on RNA Polymerase II Phosphatase Activity and Specificity - p. 267 19. Section IV: Delineating the Action of Phosphatases in Signaling and Regulation - p. 297 20. Chapter Ten: Quantitative Analysis of Intracellular Response Regulator Phosphatase Activity of Histidine Kinases - p. 297 21. Chapter Eleven: Measuring the Activities of Two-Component Regulatory System Phosphatases - p. 319 22. Chapter Twelve: Assembly of PGAM5 into Multimeric Complexes Provides a Mechanism for Allosteric Regulation of Phosphatase Activity - p. 351 23. Chapter Thirteen: Purification of Lipin and Measurement of Phosphatidic Acid Phosphatase Activity from Liposomes - p. 372 24. Chapter Fourteen: Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities - p. 388 25. Chapter Fifteen: Protein Chemical Approaches to Understanding PTEN Lipid Phosphatase Regulation - p. 403
