Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. Preface - p. xiii 7. Acknowledgments - p. xv 8. Scheduling and interrelationships of exercises - p. xvii 9. Chapter 1. History of plant cell culture - p. 1 10. Chapter 2. Setup of a tissue culture laboratory - p. 25 11. Chapter 3. Media components and preparation - p. 33 12. Chapter 4. Explant preparation - p. 47 13. Chapter 5. Contamination - p. 55 14. Chapter 6. Callus induction - p. 67 15. Chapter 7. Regeneration and morphogenesis - p. 87 16. Chapter 8. Woody shrubs and trees - p. 101 17. Chapter 9. Haploid plants from anther culture - p. 111 18. Chapter 10. Embryo rescue - p. 123 19. Chapter 11. Meristem culture for virus-free plants - p. 129 20. Chapter 12. In vitro propagation for commercial production of ornamentals - p. 137 21. Chapter 13. Protoplast isolation and fusion - p. 157 22. Agrobacterium-mediated transformation of plants - p. 171 23. Chapter 15. Genome editing and RNA interference technologies in plants - p. 195 24. Appendix I. Useful measurements - p. 213 25. Appendix II. Review of solution preparation - p. 215 26. Appendix III. List of suppliers - p. 219 27. Appendix IV. Common plant tissue culture terms - p. 221 28. Index - p. 227
Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. Preface - p. xiii 7. Acknowledgments - p. xv 8. Scheduling and interrelationships of exercises - p. xvii 9. Chapter 1. History of plant cell culture - p. 1 10. Chapter 2. Setup of a tissue culture laboratory - p. 25 11. Chapter 3. Media components and preparation - p. 33 12. Chapter 4. Explant preparation - p. 47 13. Chapter 5. Contamination - p. 55 14. Chapter 6. Callus induction - p. 67 15. Chapter 7. Regeneration and morphogenesis - p. 87 16. Chapter 8. Woody shrubs and trees - p. 101 17. Chapter 9. Haploid plants from anther culture - p. 111 18. Chapter 10. Embryo rescue - p. 123 19. Chapter 11. Meristem culture for virus-free plants - p. 129 20. Chapter 12. In vitro propagation for commercial production of ornamentals - p. 137 21. Chapter 13. Protoplast isolation and fusion - p. 157 22. Agrobacterium-mediated transformation of plants - p. 171 23. Chapter 15. Genome editing and RNA interference technologies in plants - p. 195 24. Appendix I. Useful measurements - p. 213 25. Appendix II. Review of solution preparation - p. 215 26. Appendix III. List of suppliers - p. 219 27. Appendix IV. Common plant tissue culture terms - p. 221 28. Index - p. 227
