Principles and Practice of Modern Chromatographic Methods, Second Edition takes a comprehensive, unified approach in its presentation of chromatographic techniques. Like the first edition, the book provides a scientifically rigid, but easy-to-follow presentation of chromatography concepts that begins with the purpose and intent of chromatographic theory - the “what and why that are left out of other books attempting to cover these principles. This fully revised second edition brings the content up-to-date, covering recent developments in several new sections and an additional chapter on composite methods. New topics include sample profiling, sample preparation, sustainable green chemistry, 2D chromatography, miniaturization/nano-LC, HILIC, and more.
1. Chapter 1 Introduction and overview - p. 1 2. Chapter 2 Theoretical considerations - p. 41 3. 2.1: Introduction - p. 41 4. 2.2: Theory of chromatography - p. 43 5. 2.3: Retention, dispersion, and distortion - p. 49 6. 2.4: Retention: Thermodynamics and molecular interactions - p. 55 7. 2.5: Retention - p. 56 8. 2.6: Measures of chromatographic retention - p. 62 9. 2.7: Dispersion - p. 68 10. 2.8: Is it resolution or separation? - p. 77 11. 2.9: Extra-column dispersion - p. 85 12. 2.10: Summary: Retention and dispersion - p. 89 13. 2.11: Column testing - p. 90 14. Chapter 3 Planar chromatography - p. 97 15. Chapter 4 Gas chromatography - p. 145 16. 4.1: Introduction - p. 145 17. 4.2: Mobile phases and delivery systems - p. 148 18. 4.3: Sample introduction in GC - p. 159 19. 4.4: Column packings and hardware - p. 175 20. 4.5: Detectors - p. 211 21. 4.6: Column temperature and temperature programming - p. 220 22. 4.7: Derivatization - p. 223 23. 4.8: Innovation - p. 228 24. 4.9: Separations - p. 235 25. Chapter 5 High performance liquid chromatography: Instrumentation and techniques - p. 247 26. Chapter 6 High performance liquid chromatography: Separations - p. 283 27. 6.1: Introduction - p. 283 28. 6.2: Separation of neutral compounds - p. 286 29. 6.3: Techniques for ionic and ionizable species - p. 309 30. 6.4: Specialty separation modes - p. 326 31. 6.5: Choosing a chromatographic method - p. 330 32. Chapter 7 Supercritical fluid chromatography - p. 337 33. Chapter 8 Coupled systems - p. 369 34. Chapter 9 Analyses - p. 399 35. 9.1: Introduction - p. 399 36. 9.2: Detectors for chromatography - p. 400 37. 9.3: Data handling - p. 405 38. 9.4: Qualitative analysis - p. 418 39. 9.5: Quantitative analysis - p. 425 40. 9.6: Metabolomics and chromatography - p. 434 41. Chapter 10 Sample handling in chromatography - p. 453 42. Chapter 11 Preparative chromatography - p. 495 43. Index - p. 515
Principles and Practice of Modern Chromatographic Methods, Second Edition takes a comprehensive, unified approach in its presentation of chromatographic techniques. Like the first edition, the book provides a scientifically rigid, but easy-to-follow presentation of chromatography concepts that begins with the purpose and intent of chromatographic theory - the “what and why that are left out of other books attempting to cover these principles. This fully revised second edition brings the content up-to-date, covering recent developments in several new sections and an additional chapter on composite methods. New topics include sample profiling, sample preparation, sustainable green chemistry, 2D chromatography, miniaturization/nano-LC, HILIC, and more.
1. Chapter 1 Introduction and overview - p. 1 2. Chapter 2 Theoretical considerations - p. 41 3. 2.1: Introduction - p. 41 4. 2.2: Theory of chromatography - p. 43 5. 2.3: Retention, dispersion, and distortion - p. 49 6. 2.4: Retention: Thermodynamics and molecular interactions - p. 55 7. 2.5: Retention - p. 56 8. 2.6: Measures of chromatographic retention - p. 62 9. 2.7: Dispersion - p. 68 10. 2.8: Is it resolution or separation? - p. 77 11. 2.9: Extra-column dispersion - p. 85 12. 2.10: Summary: Retention and dispersion - p. 89 13. 2.11: Column testing - p. 90 14. Chapter 3 Planar chromatography - p. 97 15. Chapter 4 Gas chromatography - p. 145 16. 4.1: Introduction - p. 145 17. 4.2: Mobile phases and delivery systems - p. 148 18. 4.3: Sample introduction in GC - p. 159 19. 4.4: Column packings and hardware - p. 175 20. 4.5: Detectors - p. 211 21. 4.6: Column temperature and temperature programming - p. 220 22. 4.7: Derivatization - p. 223 23. 4.8: Innovation - p. 228 24. 4.9: Separations - p. 235 25. Chapter 5 High performance liquid chromatography: Instrumentation and techniques - p. 247 26. Chapter 6 High performance liquid chromatography: Separations - p. 283 27. 6.1: Introduction - p. 283 28. 6.2: Separation of neutral compounds - p. 286 29. 6.3: Techniques for ionic and ionizable species - p. 309 30. 6.4: Specialty separation modes - p. 326 31. 6.5: Choosing a chromatographic method - p. 330 32. Chapter 7 Supercritical fluid chromatography - p. 337 33. Chapter 8 Coupled systems - p. 369 34. Chapter 9 Analyses - p. 399 35. 9.1: Introduction - p. 399 36. 9.2: Detectors for chromatography - p. 400 37. 9.3: Data handling - p. 405 38. 9.4: Qualitative analysis - p. 418 39. 9.5: Quantitative analysis - p. 425 40. 9.6: Metabolomics and chromatography - p. 434 41. Chapter 10 Sample handling in chromatography - p. 453 42. Chapter 11 Preparative chromatography - p. 495 43. Index - p. 515
