Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses.
1. Protocols and Applications in Enzymology - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Preface - p. Page v 7. List of Illustrations - p. Page vi 8. List of Tables - p. Page vii 9. Chapter 1 : Enzymes—past, present, and future - p. Page vii 10. Chapter 2 : Distribution and diversity in microbial enzymes - p. Page 14 11. Chapter 3 : Screening of potential microbes for enzymes of industrial significance - p. Page 39 12. Chapter 4 : Solid-state fermentation and submerged fermentation for enzyme production - p. Page 66 13. Chapter 5 : Laboratory methods for enzyme assays - p. Page 85 14. Chapter 6 : Structure and functions of enzyme kinetics - p. Page 114 15. Chapter 7 : Protocols of important industrial enzymes - p. Page 125 16. Chapter 8 : Strategies to improve enzyme activity for industrial processes - p. Page 161 17. Chapter 9 : Scope and relevance of industrial applications - p. Page 174 18. Chapter 10 : Agroindustrial wastes for enzyme production - p. Page 190 19. Chapter 11 : Significance of enzymes and their agricultural applications - p. Page 207 20. Chapter 12 : Biotechnological applications of enzymes and future prospective - p. Page 224 21. Chapter 13 : Conclusion - p. Page 237 22. Index - p. Page 242
Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses.
1. Protocols and Applications in Enzymology - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Preface - p. Page v 7. List of Illustrations - p. Page vi 8. List of Tables - p. Page vii 9. Chapter 1 : Enzymes—past, present, and future - p. Page vii 10. Chapter 2 : Distribution and diversity in microbial enzymes - p. Page 14 11. Chapter 3 : Screening of potential microbes for enzymes of industrial significance - p. Page 39 12. Chapter 4 : Solid-state fermentation and submerged fermentation for enzyme production - p. Page 66 13. Chapter 5 : Laboratory methods for enzyme assays - p. Page 85 14. Chapter 6 : Structure and functions of enzyme kinetics - p. Page 114 15. Chapter 7 : Protocols of important industrial enzymes - p. Page 125 16. Chapter 8 : Strategies to improve enzyme activity for industrial processes - p. Page 161 17. Chapter 9 : Scope and relevance of industrial applications - p. Page 174 18. Chapter 10 : Agroindustrial wastes for enzyme production - p. Page 190 19. Chapter 11 : Significance of enzymes and their agricultural applications - p. Page 207 20. Chapter 12 : Biotechnological applications of enzymes and future prospective - p. Page 224 21. Chapter 13 : Conclusion - p. Page 237 22. Index - p. Page 242
