Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical and Structural Biochemistry provides students with working knowledge of the fundamental and advanced techniques of experimental biochemistry. Sections provide an overview of the microbiological and biochemical methods typically used for the purification of metabolites and discuss the biological significance of secondary metabolites secreted by three diverse species of bacteria. Additionally, this lab manual covers the theory and practice of the most commonly-used techniques of analytical biochemistry, UV-vis and IR spectrophotometry, high-performance liquid chromatography, mass spectrometry, X-ray crystallography and nuclear magnetic resonance, and how to evaluate and effectively use scientific data.
Instructors will find this book useful because of the modular nature of the lab exercises included. Written in a logical, easy-to-understand manner, this book is an indispensable resource for both students and instructors.
1. Cover image - p. Cover 2. Title page - p. iii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. Preface - p. xv 6. Acknowledgments - p. xvii 7. Chapter 1. Working safely and efficiently in the biochemical laboratory - p. 1 8. Chapter 2. The structure and function of secondary metabolites that are secreted by bacteria - p. 9 9. Chapter 3. Overview of the methods for purification of metabolites that are secreted by bacteria - p. 19 10. Chapter 4. Absorption of ultraviolet, visible, and infrared radiation - p. 33 11. Chapter 5. High-performance liquid chromatography - p. 49 12. Chapter 6. Mass spectrometry - p. 59 13. Chapter 7. Nuclear magnetic resonance spectroscopy - p. 67 14. Chapter 8. X-ray crystallography - p. 79 15. Chapter 9. Exercises in purifying and characterizing a quorum-sensing signal - p. 85 16. Chapter 10. Exercises in purifying and characterizing iron-chelating molecules - p. 107 17. Chapter 11. Exercises in purifying and characterizing a chloroplast-targeting phytotoxin - p. 147 18. Chapter 12. Designing your own experiments - p. 173 19. Index - p. 185
Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical and Structural Biochemistry provides students with working knowledge of the fundamental and advanced techniques of experimental biochemistry. Sections provide an overview of the microbiological and biochemical methods typically used for the purification of metabolites and discuss the biological significance of secondary metabolites secreted by three diverse species of bacteria. Additionally, this lab manual covers the theory and practice of the most commonly-used techniques of analytical biochemistry, UV-vis and IR spectrophotometry, high-performance liquid chromatography, mass spectrometry, X-ray crystallography and nuclear magnetic resonance, and how to evaluate and effectively use scientific data.
Instructors will find this book useful because of the modular nature of the lab exercises included. Written in a logical, easy-to-understand manner, this book is an indispensable resource for both students and instructors.
1. Cover image - p. Cover 2. Title page - p. iii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. Preface - p. xv 6. Acknowledgments - p. xvii 7. Chapter 1. Working safely and efficiently in the biochemical laboratory - p. 1 8. Chapter 2. The structure and function of secondary metabolites that are secreted by bacteria - p. 9 9. Chapter 3. Overview of the methods for purification of metabolites that are secreted by bacteria - p. 19 10. Chapter 4. Absorption of ultraviolet, visible, and infrared radiation - p. 33 11. Chapter 5. High-performance liquid chromatography - p. 49 12. Chapter 6. Mass spectrometry - p. 59 13. Chapter 7. Nuclear magnetic resonance spectroscopy - p. 67 14. Chapter 8. X-ray crystallography - p. 79 15. Chapter 9. Exercises in purifying and characterizing a quorum-sensing signal - p. 85 16. Chapter 10. Exercises in purifying and characterizing iron-chelating molecules - p. 107 17. Chapter 11. Exercises in purifying and characterizing a chloroplast-targeting phytotoxin - p. 147 18. Chapter 12. Designing your own experiments - p. 173 19. Index - p. 185
