Quantitative Gas Chromatography-Mass Spectrometry: Principles in Life Sciences and Biochemical Applications provides an in-depth exploration of the principles and practices of GC-MS. This comprehensive guide caters to researchers, academics, and graduate students engaged in analytical chemistry, offering both theoretical knowledge and practical applications. The book is structured to introduce the fundamentals before delving into advanced techniques, ensuring a thorough understanding for all readers. The book covers essential topics such as gas chromatography-tandem mass spectrometry, liquid chromatography-tandem mass spectrometry, and the use of stable isotopes and labeled analogs. It also presents practical advice on method development, validation, and quality control.
Additionally, it addresses common issues and troubleshooting in GC-MS, making it a valuable resource for those in fields like Biochemistry, Pharmacology, Forensic Medicine, and Metabolomics.
1. 1.1 Introduction to analytical chemistry - p. 1 2. 1.2 Introduction to quantitative analysis by gas chromatography–mass spectrometry and triple quadrupole gas chromatography–mass spectrometry - p. 5 3. 1.3 Quadrupole-based gas chromatography–mass spectrometry and triple quadrupole gas chromatography–mass spectrometry - p. 9 4. 1.4 Quantitative gas chromatography–mass spectrometry and triple quadrupole gas chromatography–mass spectrometry analysis—SIM and SRM - p. 25 5. 1.5 Quantitative high-resolution mass spectrometry coupled to gas chromatography - p. 32 6. 1.6 Evaluation of the analytical performance - p. 58 7. 1.7 Comparison of quantitative analytical methods - p. 77 8. 1.8 Development and validation of quantitative gas chromatography–mass spectrometry and triple quadrupole gas chromatography–mass spectrometry methods - p. 81 9. 1.9 Ethics in analytical chemistry - p. 105 10. 1.10 Health and disease from an analytical perspective - p. 111 11. 1.11 Selection of gas chromatography–mass spectrometry instruments and columns used in the chapter - p. 147 12. 1.12 Gas chromatography–mass spectrometry in space - p. 148 13. References - p. 149 14. Further reading - p. 149 15. 2. Extraction and analytical derivatization techniques in GC–MS - p. 175 16. 3. Quantitative GC–MS analysis of amino acids - p. 305 17. 4. Quantitative GC–MS analysis of posttranslational modifications - p. 415 18. 5. Quantitative GC–MS analysis of biogenic amines and polyamines - p. 493 19. 6. Quantitative GC-MS analysis of drugs - p. 561 20. 7. Biomedical applications of GC-MS and GC-MS/MS in human studies - p. 635 21. 8. Perspectives of gas chromatography–mass spectrometry in the life sciences - p. 657 22. Abbreviations - p. 673 23. Abbreviations - p. 685 24. Subject and Names Index - p. 687
Quantitative Gas Chromatography-Mass Spectrometry: Principles in Life Sciences and Biochemical Applications provides an in-depth exploration of the principles and practices of GC-MS. This comprehensive guide caters to researchers, academics, and graduate students engaged in analytical chemistry, offering both theoretical knowledge and practical applications. The book is structured to introduce the fundamentals before delving into advanced techniques, ensuring a thorough understanding for all readers. The book covers essential topics such as gas chromatography-tandem mass spectrometry, liquid chromatography-tandem mass spectrometry, and the use of stable isotopes and labeled analogs. It also presents practical advice on method development, validation, and quality control.
Additionally, it addresses common issues and troubleshooting in GC-MS, making it a valuable resource for those in fields like Biochemistry, Pharmacology, Forensic Medicine, and Metabolomics.
1. 1.1 Introduction to analytical chemistry - p. 1 2. 1.2 Introduction to quantitative analysis by gas chromatography–mass spectrometry and triple quadrupole gas chromatography–mass spectrometry - p. 5 3. 1.3 Quadrupole-based gas chromatography–mass spectrometry and triple quadrupole gas chromatography–mass spectrometry - p. 9 4. 1.4 Quantitative gas chromatography–mass spectrometry and triple quadrupole gas chromatography–mass spectrometry analysis—SIM and SRM - p. 25 5. 1.5 Quantitative high-resolution mass spectrometry coupled to gas chromatography - p. 32 6. 1.6 Evaluation of the analytical performance - p. 58 7. 1.7 Comparison of quantitative analytical methods - p. 77 8. 1.8 Development and validation of quantitative gas chromatography–mass spectrometry and triple quadrupole gas chromatography–mass spectrometry methods - p. 81 9. 1.9 Ethics in analytical chemistry - p. 105 10. 1.10 Health and disease from an analytical perspective - p. 111 11. 1.11 Selection of gas chromatography–mass spectrometry instruments and columns used in the chapter - p. 147 12. 1.12 Gas chromatography–mass spectrometry in space - p. 148 13. References - p. 149 14. Further reading - p. 149 15. 2. Extraction and analytical derivatization techniques in GC–MS - p. 175 16. 3. Quantitative GC–MS analysis of amino acids - p. 305 17. 4. Quantitative GC–MS analysis of posttranslational modifications - p. 415 18. 5. Quantitative GC–MS analysis of biogenic amines and polyamines - p. 493 19. 6. Quantitative GC-MS analysis of drugs - p. 561 20. 7. Biomedical applications of GC-MS and GC-MS/MS in human studies - p. 635 21. 8. Perspectives of gas chromatography–mass spectrometry in the life sciences - p. 657 22. Abbreviations - p. 673 23. Abbreviations - p. 685 24. Subject and Names Index - p. 687
