Retinoid Signaling Pathways, Volume 637, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sections in this release include The chemistry and biochemistry of Vitamin A and its natural derivative, Biosynthesis of retinoic acids, Biodegration of retinoic acids mediated by retinoid binding proteins, Retinoic acid homeostasis, Cryo Electron Microscopy to study retinol update via the STRA6 receptor, Immuno-detection of retinoic acid synthesis enzymes in the brain, classical pathway of gene regulation by retinoids, Protein-protein interactions in the regulation of retinoid acid receptors activity, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xiii 6. Preface - p. page_xix 7. Chapter One: Vitamin A and its natural derivatives - p. page_xx 8. Chapter Two: Post-natal all-trans-retinoic acid biosynthesis - p. page_25 9. Chapter Three: Retinoid metabolism and functions mediated by retinoid binding-proteins - p. page_54 10. Chapter Four: Generation and isolation of recombinant retinoid oxidoreductase complex - p. page_75 11. Chapter Five: Sample preparation for structural and functional analyses of the STRA6 receptor for retinol-binding protein - p. page_93 12. Chapter Six: Use of fixatives for immunohistochemistry and their application for detection of retinoic acid synthesizing enzymes in the central nervous system - p. page_116 13. Chapter Seven: Classical pathways of gene regulation by retinoids - p. page_150 14. Chapter Eight: Protein-protein interactions in the regulation of RAR–RXR heterodimers transcriptional activity - p. page_173 15. Chapter Nine: Natural ligands of RXR receptors - p. page_207 16. Chapter Ten: DNA recognition by retinoic acid nuclear receptors - p. page_234 17. Chapter Eleven: Noncanonical retinoic acid signaling - p. page_260 18. Chapter Twelve: Retinoic acid and microRNA - p. page_281 19. Chapter Thirteen: Analysis of vitamin A and retinoids in biological matrices - p. page_308 20. Chapter Fourteen: Effects of vitamin A and retinoic acid on mouse embryonic stem cells and their differentiating progeny - p. page_340 21. Chapter Fifteen: Identifying vitamin A signaling by visualizing gene and protein activity, and by quantification of vitamin A metabolites - p. page_365 22. Chapter Sixteen: Whole mount in situ hybridization and immunohistochemistry for studying retinoic acid signaling in developing amphioxus - p. page_418 23. Chapter Seventeen: Design of synthetic retinoids - p. page_452 24. Chapter Eighteen: Application of human organotypic skin raft cultures for analysis of retinoid metabolism, retinoic acid signaling, and screening of bioactive rexinoids - p. page_491 25. Chapter Nineteen: Tissue localization of retinoic acid receptor (RAR) active drugs - p. page_512 26. Chapter Twenty: The development of methodologies for high-throughput retinoic acid binding assays in drug discovery and beyond - p. page_538 27. Chapter Twenty-One: Using the human CYP26A1 gene promoter as a suitable tool for the determination of RAR-mediated retinoid activity - p. page_560
Retinoid Signaling Pathways, Volume 637, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sections in this release include The chemistry and biochemistry of Vitamin A and its natural derivative, Biosynthesis of retinoic acids, Biodegration of retinoic acids mediated by retinoid binding proteins, Retinoic acid homeostasis, Cryo Electron Microscopy to study retinol update via the STRA6 receptor, Immuno-detection of retinoic acid synthesis enzymes in the brain, classical pathway of gene regulation by retinoids, Protein-protein interactions in the regulation of retinoid acid receptors activity, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xiii 6. Preface - p. page_xix 7. Chapter One: Vitamin A and its natural derivatives - p. page_xx 8. Chapter Two: Post-natal all-trans-retinoic acid biosynthesis - p. page_25 9. Chapter Three: Retinoid metabolism and functions mediated by retinoid binding-proteins - p. page_54 10. Chapter Four: Generation and isolation of recombinant retinoid oxidoreductase complex - p. page_75 11. Chapter Five: Sample preparation for structural and functional analyses of the STRA6 receptor for retinol-binding protein - p. page_93 12. Chapter Six: Use of fixatives for immunohistochemistry and their application for detection of retinoic acid synthesizing enzymes in the central nervous system - p. page_116 13. Chapter Seven: Classical pathways of gene regulation by retinoids - p. page_150 14. Chapter Eight: Protein-protein interactions in the regulation of RAR–RXR heterodimers transcriptional activity - p. page_173 15. Chapter Nine: Natural ligands of RXR receptors - p. page_207 16. Chapter Ten: DNA recognition by retinoic acid nuclear receptors - p. page_234 17. Chapter Eleven: Noncanonical retinoic acid signaling - p. page_260 18. Chapter Twelve: Retinoic acid and microRNA - p. page_281 19. Chapter Thirteen: Analysis of vitamin A and retinoids in biological matrices - p. page_308 20. Chapter Fourteen: Effects of vitamin A and retinoic acid on mouse embryonic stem cells and their differentiating progeny - p. page_340 21. Chapter Fifteen: Identifying vitamin A signaling by visualizing gene and protein activity, and by quantification of vitamin A metabolites - p. page_365 22. Chapter Sixteen: Whole mount in situ hybridization and immunohistochemistry for studying retinoic acid signaling in developing amphioxus - p. page_418 23. Chapter Seventeen: Design of synthetic retinoids - p. page_452 24. Chapter Eighteen: Application of human organotypic skin raft cultures for analysis of retinoid metabolism, retinoic acid signaling, and screening of bioactive rexinoids - p. page_491 25. Chapter Nineteen: Tissue localization of retinoic acid receptor (RAR) active drugs - p. page_512 26. Chapter Twenty: The development of methodologies for high-throughput retinoic acid binding assays in drug discovery and beyond - p. page_538 27. Chapter Twenty-One: Using the human CYP26A1 gene promoter as a suitable tool for the determination of RAR-mediated retinoid activity - p. page_560
