RNA Enzymology and Technology, Volume 725 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as RNA enzymes in transcription, RNA processing, translation regulation, RNA transcriptase in gene expression regulation, Enzymes Involved in Ribosome Biogenesis and Translation Regulation, In Vitro Transcriptase: Methods for Studying RNA Synthesis, RNA Enzymes in RNA Silencing and Regulation, RNA Silencing Machinery: Mechanisms of RISC and MicroRNA Regulation, Enzymes in RNA-Based Therapeutics, and much more.
Other chapters cover Antisense Oligonucleotides (ASOs) and RNase H: Mechanisms and Applications, RNA Enzymes in Targeted Therapeutics: From Bench to Bedside, Enzymes in RNA Sequencing and Analysis, Enzymes in High-Throughput RNA Sequencing: Applications and Challenges, Advanced Techniques and Future Directions, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xv 8. Section 1: RNA enzymes in transcription, RNA processing, and translation regulation - p. 1 9. Chapter One: Unmasking a hidden code during transcription: A practical strategy to reveal Thr4 phosphorylation in RNA polymerase II - p. 3 10. Chapter Two: In Vitro transcriptase: Methods for studying XNA synthesis - p. 33 11. Chapter Three: Enzymes in high-throughput RNA sequencing: Applications and challenges - p. 51 12. Chapter Four: An smFRET assay to probe the impact of antibiotics on intersubunit rotation in eukaryotic ribosomes - p. 77 13. Chapter Five: A non-radioactive method to detect and measure 48S initiation complex and 80S ribosome formation in vitro - p. 105 14. Section 2: Enzymes in RNA Silencing and Regulation - p. 125 15. Chapter Six: Bioinformatic identification of regulatory feedback motifs within RNAi pathways using multi-omics datasets - p. 127 16. Chapter Seven: Induro-seq to analyze subcellular enrichment of small RNAs - p. 155 17. Section 3: Enzymes in RNA-Based Technologies - p. 175 18. Chapter Eight: Preparation of enzymes and libraries for MapID-tRNA-seq to identify chemical modifications in human tRNAs - p. 177 19. Section 4: RNA Modification and Structural Analysis - p. 223 20. Chapter Nine: m6A Immunoprecipitation from Ribosome-Bound mRNA - p. 225 21. Chapter Ten: Synthesis and biochemical studies of N3-methylcytidine(m3C), N4-methylcytidine (m4C) and N4, N4-dimethylcytidine (m42C) modified RNAs - p. 255 22. Chapter Eleven: Detecting tRNA modifications with fluorescent-labeled DNA probes - p. 283 23. Chapter Twelve: RNA structure analyzed by chemical probing and mutational profiling with sequencing (MaPseq) - p. 301
RNA Enzymology and Technology, Volume 725 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as RNA enzymes in transcription, RNA processing, translation regulation, RNA transcriptase in gene expression regulation, Enzymes Involved in Ribosome Biogenesis and Translation Regulation, In Vitro Transcriptase: Methods for Studying RNA Synthesis, RNA Enzymes in RNA Silencing and Regulation, RNA Silencing Machinery: Mechanisms of RISC and MicroRNA Regulation, Enzymes in RNA-Based Therapeutics, and much more.
Other chapters cover Antisense Oligonucleotides (ASOs) and RNase H: Mechanisms and Applications, RNA Enzymes in Targeted Therapeutics: From Bench to Bedside, Enzymes in RNA Sequencing and Analysis, Enzymes in High-Throughput RNA Sequencing: Applications and Challenges, Advanced Techniques and Future Directions, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xv 8. Section 1: RNA enzymes in transcription, RNA processing, and translation regulation - p. 1 9. Chapter One: Unmasking a hidden code during transcription: A practical strategy to reveal Thr4 phosphorylation in RNA polymerase II - p. 3 10. Chapter Two: In Vitro transcriptase: Methods for studying XNA synthesis - p. 33 11. Chapter Three: Enzymes in high-throughput RNA sequencing: Applications and challenges - p. 51 12. Chapter Four: An smFRET assay to probe the impact of antibiotics on intersubunit rotation in eukaryotic ribosomes - p. 77 13. Chapter Five: A non-radioactive method to detect and measure 48S initiation complex and 80S ribosome formation in vitro - p. 105 14. Section 2: Enzymes in RNA Silencing and Regulation - p. 125 15. Chapter Six: Bioinformatic identification of regulatory feedback motifs within RNAi pathways using multi-omics datasets - p. 127 16. Chapter Seven: Induro-seq to analyze subcellular enrichment of small RNAs - p. 155 17. Section 3: Enzymes in RNA-Based Technologies - p. 175 18. Chapter Eight: Preparation of enzymes and libraries for MapID-tRNA-seq to identify chemical modifications in human tRNAs - p. 177 19. Section 4: RNA Modification and Structural Analysis - p. 223 20. Chapter Nine: m6A Immunoprecipitation from Ribosome-Bound mRNA - p. 225 21. Chapter Ten: Synthesis and biochemical studies of N3-methylcytidine(m3C), N4-methylcytidine (m4C) and N4, N4-dimethylcytidine (m42C) modified RNAs - p. 255 22. Chapter Eleven: Detecting tRNA modifications with fluorescent-labeled DNA probes - p. 283 23. Chapter Twelve: RNA structure analyzed by chemical probing and mutational profiling with sequencing (MaPseq) - p. 301
