RNA Modification Enzymes, Volume 659 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of related topics, including Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis, Development of RNA modification mapping pipelines for high-throughput sequencing approaches, AlkAniline-Seq for high-resolution mapping RNA m7G and m3C modifications, Facile detection of RNA phospho-methylation in cells, Detection and analysis of glycosylated queuosine modifications, A comprehensive pipeline for analysis of RNA 3’-end modification, Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry, and more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Tables - p. iii 5. 21 - p. iii 6. Copyright - p. iii 7. Contributors - p. iii 8. Preface - p. iii 9. Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis - p. iii 10. Mapping of 7-methylguanosine (m7G), 3-methylcytidine (m3C), dihydrouridine (D) and 5-hydroxycytidine (ho5C) RNA modifications by AlkAniline-Seq - p. iii 11. Facile detection of RNA phospho-methylation in cells and tissues - p. iii 12. Quantitative probing of glycosylated queuosine modifications in tRNA - p. iii 13. CTS tag-based methods for investigating mitochondrial RNA modification factors in Trypanosoma brucei - p. iii 14. Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry - p. iii 15. RNA immunoprecipitation to identify in vivo targets of RNA editing and modifying enzymes - p. iii 16. Chemoenzymatic labeling of RNA to enrich, detect and identify methyltransferase-target sites - p. iii 17. Analysis of codon-specific translation by ribosome profiling - p. iii 18. Partially modified tRNAs for the study of tRNA maturation and function - p. iii 19. Transient kinetic analysis for studying ionizations in RNA modification enzyme mechanisms - p. iii 20. Pseudouridine site assignment by high-throughput in vitro RNA pseudouridylation and sequencing - p. iii 21. Detection of tRNA-specific adenosine deaminase activity and wobble inosine modification in human cell lysates - p. iii 22. In vitro and in cellula site-directed RNA editing using the λNDD-BoxB system - p. iii 23. A semi-quantitative pull-down assay to study tRNA substrate specificity of modification enzymes - p. iii 24. Investigating the consequences of mRNA modifications on protein synthesis using in vitro translation assays - p. iii 25. Mass spectrometric analysis of mRNA 5′ terminal modifications - p. iii 26. CLIP-Seq to identify targets and interactions of RNA binding proteins and RNA modifying enzymes - p. iii 27. CRISPR mediated genome editing, a tool to dissect RNA modification processes - p. iii
RNA Modification Enzymes, Volume 659 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of related topics, including Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis, Development of RNA modification mapping pipelines for high-throughput sequencing approaches, AlkAniline-Seq for high-resolution mapping RNA m7G and m3C modifications, Facile detection of RNA phospho-methylation in cells, Detection and analysis of glycosylated queuosine modifications, A comprehensive pipeline for analysis of RNA 3’-end modification, Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry, and more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Tables - p. iii 5. 21 - p. iii 6. Copyright - p. iii 7. Contributors - p. iii 8. Preface - p. iii 9. Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis - p. iii 10. Mapping of 7-methylguanosine (m7G), 3-methylcytidine (m3C), dihydrouridine (D) and 5-hydroxycytidine (ho5C) RNA modifications by AlkAniline-Seq - p. iii 11. Facile detection of RNA phospho-methylation in cells and tissues - p. iii 12. Quantitative probing of glycosylated queuosine modifications in tRNA - p. iii 13. CTS tag-based methods for investigating mitochondrial RNA modification factors in Trypanosoma brucei - p. iii 14. Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry - p. iii 15. RNA immunoprecipitation to identify in vivo targets of RNA editing and modifying enzymes - p. iii 16. Chemoenzymatic labeling of RNA to enrich, detect and identify methyltransferase-target sites - p. iii 17. Analysis of codon-specific translation by ribosome profiling - p. iii 18. Partially modified tRNAs for the study of tRNA maturation and function - p. iii 19. Transient kinetic analysis for studying ionizations in RNA modification enzyme mechanisms - p. iii 20. Pseudouridine site assignment by high-throughput in vitro RNA pseudouridylation and sequencing - p. iii 21. Detection of tRNA-specific adenosine deaminase activity and wobble inosine modification in human cell lysates - p. iii 22. In vitro and in cellula site-directed RNA editing using the λNDD-BoxB system - p. iii 23. A semi-quantitative pull-down assay to study tRNA substrate specificity of modification enzymes - p. iii 24. Investigating the consequences of mRNA modifications on protein synthesis using in vitro translation assays - p. iii 25. Mass spectrometric analysis of mRNA 5′ terminal modifications - p. iii 26. CLIP-Seq to identify targets and interactions of RNA binding proteins and RNA modifying enzymes - p. iii 27. CRISPR mediated genome editing, a tool to dissect RNA modification processes - p. iii
