Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Contributors - p. xv 7. Foreword - p. xix 8. Part 1: Theory - p. xx 9. Chapter 1. Coherent Raman scattering processes - p. 3 10. Chapter 2. Sensitivity and noise in SRS microscopy - p. 21 11. Chapter 3. Stimulated Raman scattering: Ensembles to single molecules - p. 41 12. Part 2: Advanced Instrumentation and emerging modalities - p. 65 13. Chapter 4. Hyperspectral SRS imaging via spectral focusing - p. 69 14. Chapter 5. Balanced detection SRS microscopy - p. 81 15. Chapter 6. Multiplex stimulated Raman scattering microscopy via a tuned amplifier - p. 91 16. Chapter 7. Impulsive SRS microscopy - p. 99 17. Chapter 8. Multicolor SRS imaging with wavelength-tunable/switchable lasers - p. 115 18. Chapter 9. Pulse-shaping-based SRS spectral imaging and applications - p. 127 19. Chapter 10. Background-free stimulated Raman scattering imaging by manipulating photons in the spectral domain - p. 137 20. Chapter 11. Coherent Raman scattering microscopy for superresolution vibrational imaging: Principles, techniques, and implementations - p. 147 21. Chapter 12. Quantum-enhanced stimulated Raman scattering - p. 165 22. Chapter 13. Stimulated Raman excited fluorescence (SREF) microscopy: Combining the best of two worlds - p. 179 23. Chapter 14. Instrumentation and methodology for volumetric stimulated Raman scattering imaging - p. 189 24. Chapter 15. SRS flow and image cytometry - p. 203 25. Chapter 16. Widely and rapidly tunable fiber laser for high-speed multicolor SRS - p. 215 26. Chapter 17. Compact fiber lasers for stimulated Raman scattering microscopy - p. 233 27. Chapter 18. Synchronized time-lens source for coherent Raman scattering microscopy - p. 257 28. Part 3: Vibrational probes - p. 271 29. Chapter 19. Spontaneous Raman and SERS microscopy for Raman tag imaging - p. 275 30. Chapter 20. Stimulated Raman scattering imaging with small vibrational probes - p. 289 31. Chapter 21. Supermultiplexed vibrational imaging: From probe development to biomedical applications - p. 311 32. Chapter 22. Raman beads for bio-imaging - p. 329 33. Chapter 23. Plasmon-enhanced stimulated Raman scattering microscopy - p. 343 34. Part 4: Data science - p. 356 35. Chapter 24. Converting hyperspectral SRS into chemical maps - p. 359 36. Chapter 25. Compressive Raman microspectroscopy - p. 371 37. Chapter 26. Denoise SRS images - p. 383 38. Part 5: Applications to life science and materials science - p. 399 39. Chapter 27. Use of SRS microscopy for imaging drugs - p. 403 40. Chapter 28. Isotope-probed SRS (ip-SRS) imaging of metabolic dynamics in living organisms - p. 421 41. Chapter 29. Rapid determination of antimicrobial susceptibility by SRS single-cell metabolic imaging - p. 445 42. Chapter 30. Stimulated Raman scattering imaging of cancer metabolism: New avenue to precision medicine - p. 463 43. Chapter 31. Biomedical applications of SRS microscopy in functional genetics and genomics - p. 475 44. Chapter 32. Stimulated Raman voltage imaging for quantitative mapping of membrane potential - p. 487 45. Chapter 33. Neurodegenerative disease by SRS microscopy - p. 501 46. Chapter 34. Applications of stimulated Raman scattering (SRS) microscopy in materials science - p. 515 47. Chapter 35. Resolving molecular orientation by polarization-sensitive stimulated Raman scattering microscopy - p. 529 48. Part 6: Miniaturization and translation to medicine - p. 537 49. Chapter 36. Stimulated Raman histology - p. 541 50. Chapter 37. Miniaturized handheld stimulated Raman scattering microscope - p. 551 51. Chapter 38. Intraoperative multimodal imaging - p. 561 52. Index - p. 583
Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Contributors - p. xv 7. Foreword - p. xix 8. Part 1: Theory - p. xx 9. Chapter 1. Coherent Raman scattering processes - p. 3 10. Chapter 2. Sensitivity and noise in SRS microscopy - p. 21 11. Chapter 3. Stimulated Raman scattering: Ensembles to single molecules - p. 41 12. Part 2: Advanced Instrumentation and emerging modalities - p. 65 13. Chapter 4. Hyperspectral SRS imaging via spectral focusing - p. 69 14. Chapter 5. Balanced detection SRS microscopy - p. 81 15. Chapter 6. Multiplex stimulated Raman scattering microscopy via a tuned amplifier - p. 91 16. Chapter 7. Impulsive SRS microscopy - p. 99 17. Chapter 8. Multicolor SRS imaging with wavelength-tunable/switchable lasers - p. 115 18. Chapter 9. Pulse-shaping-based SRS spectral imaging and applications - p. 127 19. Chapter 10. Background-free stimulated Raman scattering imaging by manipulating photons in the spectral domain - p. 137 20. Chapter 11. Coherent Raman scattering microscopy for superresolution vibrational imaging: Principles, techniques, and implementations - p. 147 21. Chapter 12. Quantum-enhanced stimulated Raman scattering - p. 165 22. Chapter 13. Stimulated Raman excited fluorescence (SREF) microscopy: Combining the best of two worlds - p. 179 23. Chapter 14. Instrumentation and methodology for volumetric stimulated Raman scattering imaging - p. 189 24. Chapter 15. SRS flow and image cytometry - p. 203 25. Chapter 16. Widely and rapidly tunable fiber laser for high-speed multicolor SRS - p. 215 26. Chapter 17. Compact fiber lasers for stimulated Raman scattering microscopy - p. 233 27. Chapter 18. Synchronized time-lens source for coherent Raman scattering microscopy - p. 257 28. Part 3: Vibrational probes - p. 271 29. Chapter 19. Spontaneous Raman and SERS microscopy for Raman tag imaging - p. 275 30. Chapter 20. Stimulated Raman scattering imaging with small vibrational probes - p. 289 31. Chapter 21. Supermultiplexed vibrational imaging: From probe development to biomedical applications - p. 311 32. Chapter 22. Raman beads for bio-imaging - p. 329 33. Chapter 23. Plasmon-enhanced stimulated Raman scattering microscopy - p. 343 34. Part 4: Data science - p. 356 35. Chapter 24. Converting hyperspectral SRS into chemical maps - p. 359 36. Chapter 25. Compressive Raman microspectroscopy - p. 371 37. Chapter 26. Denoise SRS images - p. 383 38. Part 5: Applications to life science and materials science - p. 399 39. Chapter 27. Use of SRS microscopy for imaging drugs - p. 403 40. Chapter 28. Isotope-probed SRS (ip-SRS) imaging of metabolic dynamics in living organisms - p. 421 41. Chapter 29. Rapid determination of antimicrobial susceptibility by SRS single-cell metabolic imaging - p. 445 42. Chapter 30. Stimulated Raman scattering imaging of cancer metabolism: New avenue to precision medicine - p. 463 43. Chapter 31. Biomedical applications of SRS microscopy in functional genetics and genomics - p. 475 44. Chapter 32. Stimulated Raman voltage imaging for quantitative mapping of membrane potential - p. 487 45. Chapter 33. Neurodegenerative disease by SRS microscopy - p. 501 46. Chapter 34. Applications of stimulated Raman scattering (SRS) microscopy in materials science - p. 515 47. Chapter 35. Resolving molecular orientation by polarization-sensitive stimulated Raman scattering microscopy - p. 529 48. Part 6: Miniaturization and translation to medicine - p. 537 49. Chapter 36. Stimulated Raman histology - p. 541 50. Chapter 37. Miniaturized handheld stimulated Raman scattering microscope - p. 551 51. Chapter 38. Intraoperative multimodal imaging - p. 561 52. Index - p. 583
