Synthetic Biology and Metabolic Engineering in Plants and Microbes, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the usage of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Chapter One: Comparative Biochemistry and In Vitro Pathway Reconstruction as Powerful Partners in Studies of Metabolic Diversity - p. xviii 8. Chapter Two: De Novo Deep Transcriptome Analysis of Medicinal Plants for Gene Discovery in Biosynthesis of Plant Natural Products - p. 17 9. Chapter Three: Genomics-Based Discovery of Plant Genes for Synthetic Biology of Terpenoid Fragrances: A Case Study in Sandalwood oil Biosynthesis - p. 45 10. Chapter Four: A Workflow for Studying Specialized Metabolism in Nonmodel Eukaryotic Organisms - p. 67 11. Chapter Five: Gene Discovery for Synthetic Biology: Exploring the Novel Natural Product Biosynthetic Capacity of Eukaryotic Microalgae - p. 97 12. Chapter Six: cis-Prenyltransferase and Polymer Analysis from a Natural Rubber Perspective - p. 120 13. Chapter Seven: Generation and Functional Evaluation of Designer Monoterpene Synthases - p. 145 14. Chapter Eight: Prequels to Synthetic Biology: From Candidate Gene Identification and Validation to Enzyme Subcellular Localization in Plant and Yeast Cells - p. 165 15. Chapter Nine: Functional Expression and Characterization of Plant ABC Transporters in Xenopus laevis Oocytes for Transport Engineering Purposes - p. 206 16. Chapter Ten: Quantifying the Metabolites of the Methylerythritol 4-Phosphate (MEP) Pathway in Plants and Bacteria by Liquid Chromatography–Triple Quadrupole Mass Spectrometry - p. 224 17. Chapter Eleven: Establishing the Architecture of Plant Gene Regulatory Networks - p. 249 18. Chapter Twelve: Engineering of Tomato Glandular Trichomes for the Production of Specialized Metabolites - p. 304 19. Chapter Thirteen: Tomato Fruits—A Platform for Metabolic Engineering of Terpenes - p. 331 20. Chapter Fourteen: Libraries of Synthetic TALE-Activated Promoters: Methods and Applications - p. 359 21. Author Index - p. 379 22. Subject Index - p. 419
Synthetic Biology and Metabolic Engineering in Plants and Microbes, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the usage of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Chapter One: Comparative Biochemistry and In Vitro Pathway Reconstruction as Powerful Partners in Studies of Metabolic Diversity - p. xviii 8. Chapter Two: De Novo Deep Transcriptome Analysis of Medicinal Plants for Gene Discovery in Biosynthesis of Plant Natural Products - p. 17 9. Chapter Three: Genomics-Based Discovery of Plant Genes for Synthetic Biology of Terpenoid Fragrances: A Case Study in Sandalwood oil Biosynthesis - p. 45 10. Chapter Four: A Workflow for Studying Specialized Metabolism in Nonmodel Eukaryotic Organisms - p. 67 11. Chapter Five: Gene Discovery for Synthetic Biology: Exploring the Novel Natural Product Biosynthetic Capacity of Eukaryotic Microalgae - p. 97 12. Chapter Six: cis-Prenyltransferase and Polymer Analysis from a Natural Rubber Perspective - p. 120 13. Chapter Seven: Generation and Functional Evaluation of Designer Monoterpene Synthases - p. 145 14. Chapter Eight: Prequels to Synthetic Biology: From Candidate Gene Identification and Validation to Enzyme Subcellular Localization in Plant and Yeast Cells - p. 165 15. Chapter Nine: Functional Expression and Characterization of Plant ABC Transporters in Xenopus laevis Oocytes for Transport Engineering Purposes - p. 206 16. Chapter Ten: Quantifying the Metabolites of the Methylerythritol 4-Phosphate (MEP) Pathway in Plants and Bacteria by Liquid Chromatography–Triple Quadrupole Mass Spectrometry - p. 224 17. Chapter Eleven: Establishing the Architecture of Plant Gene Regulatory Networks - p. 249 18. Chapter Twelve: Engineering of Tomato Glandular Trichomes for the Production of Specialized Metabolites - p. 304 19. Chapter Thirteen: Tomato Fruits—A Platform for Metabolic Engineering of Terpenes - p. 331 20. Chapter Fourteen: Libraries of Synthetic TALE-Activated Promoters: Methods and Applications - p. 359 21. Author Index - p. 379 22. Subject Index - p. 419
