Targeted Protein Degradation, Volume 680 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, with each. Each written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xv 8. Chapter One: High-efficiency knock-in of degradable tags (dTAG) at endogenous loci in cell lines - p. 1 9. Chapter Two: High throughput E3 ligase degron binding assays for novel PROTAC ligand discovery - p. 23 10. Chapter Three: Synthesis of SNIPERs against BCR-ABL with kinase inhibitors and a method to evaluate their growth inhibitory activity derived from BCR-ABL degradation - p. 41 11. Chapter Four: Harnessing nanobodies for target protein degradation through the Affinity-directed PROtein Missile (AdPROM) system - p. 61 12. Chapter Five: Monitoring PROTAC interactions in biochemical assays using Lumit immunoassays - p. 81 13. Chapter Six: The In-Cell Western immunofluorescence assay to monitor PROTAC mediated protein degradation - p. 115 14. Chapter Seven: Cereblon target validation using a covalent inhibitor of neosubstrate recruitment - p. 155 15. Chapter Eight: Advancing targeted protein degrader discovery by measuring cereblon engagement in cells - p. 169 16. Chapter Nine: Quantitative measurement of PROTAC intracellular accumulation - p. 189 17. Chapter Ten: The importance of controls in targeted protein degradation: Determining mechanism - p. 215 18. Chapter Eleven: Crystallization of VHL-based PROTAC-induced ternary complexes - p. 241 19. Chapter Twelve: Targeted protein degradation through light-activated E3 ligase recruitment - p. 265 20. Chapter Thirteen: A heterobifunctional molecule system for targeted protein acetylation in cells - p. 287
Targeted Protein Degradation, Volume 680 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, with each. Each written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xv 8. Chapter One: High-efficiency knock-in of degradable tags (dTAG) at endogenous loci in cell lines - p. 1 9. Chapter Two: High throughput E3 ligase degron binding assays for novel PROTAC ligand discovery - p. 23 10. Chapter Three: Synthesis of SNIPERs against BCR-ABL with kinase inhibitors and a method to evaluate their growth inhibitory activity derived from BCR-ABL degradation - p. 41 11. Chapter Four: Harnessing nanobodies for target protein degradation through the Affinity-directed PROtein Missile (AdPROM) system - p. 61 12. Chapter Five: Monitoring PROTAC interactions in biochemical assays using Lumit immunoassays - p. 81 13. Chapter Six: The In-Cell Western immunofluorescence assay to monitor PROTAC mediated protein degradation - p. 115 14. Chapter Seven: Cereblon target validation using a covalent inhibitor of neosubstrate recruitment - p. 155 15. Chapter Eight: Advancing targeted protein degrader discovery by measuring cereblon engagement in cells - p. 169 16. Chapter Nine: Quantitative measurement of PROTAC intracellular accumulation - p. 189 17. Chapter Ten: The importance of controls in targeted protein degradation: Determining mechanism - p. 215 18. Chapter Eleven: Crystallization of VHL-based PROTAC-induced ternary complexes - p. 241 19. Chapter Twelve: Targeted protein degradation through light-activated E3 ligase recruitment - p. 265 20. Chapter Thirteen: A heterobifunctional molecule system for targeted protein acetylation in cells - p. 287
