Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more. The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.
1. Chapter 1 : Introduction to biochemistry - p. Page ix 2. Chapter 2 : Introduction to laboratory procedures - p. Page 5 3. Chapter 3 : Preparation of solutions and buffers - p. Page 11 4. Chapter 4 : Qualitative analysis of carbohydrates - p. Page 18 5. Chapter 5 : Qualitative analysis of proteins - p. Page 28 6. Chapter 6 : pH (negative logarithm of hydrogen ion concentration) - p. Page 36 7. Chapter 7 : Spectrophotometry - p. Page 40 8. Chapter 8 : Estimation of carbohydrates - p. Page 46 9. Chapter 9 : Amino acids and proteins - p. Page 59 10. Chapter 10 : Enzyme assays - p. Page 68 11. Chapter 11 : Vitamin assays - p. Page 98 12. Chapter 12 : Phenolics - p. Page 116 13. Chapter 13 : Plant pigments - p. Page 125 14. Chapter 14 : Plant acids - p. Page 133 15. Chapter 15 : Antinutritional factors in plants - p. Page 137 16. Chapter 16 : Toxins - p. Page 144 17. Chapter 17 : Extraction and identification of polyacetylenes - p. Page 147 18. Chapter 18 : Alkaloids - p. Page 150 19. Chapter 19 : Plant hormones - p. Page 152 20. Chapter 20 : Immunochemical methods - p. Page 162 21. Chapter 21 : Nitrogenase activity - p. Page 165 22. Chapter 22 : Chlorophyll - p. Page 169 23. Chapter 23 : Proximate analysis - p. Page 172 24. Chapter 24 : Minerals - p. Page 179 25. Chapter 25 : Antinutritional factors in plants - p. Page 203 26. Chapter 26 : Antioxidants - p. Page 209 27. Chapter 27 : Lipids - p. Page 214 28. Index - p. Page 218
Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more. The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.
1. Chapter 1 : Introduction to biochemistry - p. Page ix 2. Chapter 2 : Introduction to laboratory procedures - p. Page 5 3. Chapter 3 : Preparation of solutions and buffers - p. Page 11 4. Chapter 4 : Qualitative analysis of carbohydrates - p. Page 18 5. Chapter 5 : Qualitative analysis of proteins - p. Page 28 6. Chapter 6 : pH (negative logarithm of hydrogen ion concentration) - p. Page 36 7. Chapter 7 : Spectrophotometry - p. Page 40 8. Chapter 8 : Estimation of carbohydrates - p. Page 46 9. Chapter 9 : Amino acids and proteins - p. Page 59 10. Chapter 10 : Enzyme assays - p. Page 68 11. Chapter 11 : Vitamin assays - p. Page 98 12. Chapter 12 : Phenolics - p. Page 116 13. Chapter 13 : Plant pigments - p. Page 125 14. Chapter 14 : Plant acids - p. Page 133 15. Chapter 15 : Antinutritional factors in plants - p. Page 137 16. Chapter 16 : Toxins - p. Page 144 17. Chapter 17 : Extraction and identification of polyacetylenes - p. Page 147 18. Chapter 18 : Alkaloids - p. Page 150 19. Chapter 19 : Plant hormones - p. Page 152 20. Chapter 20 : Immunochemical methods - p. Page 162 21. Chapter 21 : Nitrogenase activity - p. Page 165 22. Chapter 22 : Chlorophyll - p. Page 169 23. Chapter 23 : Proximate analysis - p. Page 172 24. Chapter 24 : Minerals - p. Page 179 25. Chapter 25 : Antinutritional factors in plants - p. Page 203 26. Chapter 26 : Antioxidants - p. Page 209 27. Chapter 27 : Lipids - p. Page 214 28. Index - p. Page 218
