As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.
1. Cover 2. Title Page 3. Copyright 4. Contributors 5. Preface 6. Part I: Practical Considerations and Equipment 7. Chapter 1: Theoretical Basis of Confocal Microscopy 8. Chapter 2: Practical Considerations in Acquiring Biological Signals from Confocal Microscope 9. Chapter 3: Equipment for Mass Storage and Processing of Data 10. Chapter 4: Antifading Agents for Confocal Fluorescence Microscopy 11. Chapter 5: Mounting Techniques for Confocal Microscopy 12. Chapter 6: Preparation of Whole Mounts and Thick Sections for Confocal Microscopy 13. Chapter 7: Use of Confocal Microscopy to Investigate Cell Structure and Function 14. Chapter 8: Combining Laser Scanning Confocal Microscopy and Electron Microscopy 15. Part II: Functional Approaches 16. Chapter 9: Volume Measurements in Confocal Microscopy 17. Chapter 10: Quantitation of Phagocytosis by Confocal Microscopy 18. Chapter 11: Receptor–Ligand Internalization 19. Chapter 12: Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy 20. Chapter 13: Trafficking of the Androgen Receptor 21. Chapter 14: Confocal Microscopy 22. Chapter 15: Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues; Functional Metabolic Imaging of Human Skin In Vivo 23. Chapter 16: Video-Rate, Scanning Slit Confocal Microscopy of Living Human Cornea In Vivo 24. Chapter 17: In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) Method 25. Chapter 18: Identification of Viral Infection by Confocal Microscopy 26. Chapter 19: Membrane Trafficking 27. Part III: Green Fluorescent Protein 28. Chapter 20: Monitoring of Protein Secretion with Green Fluorescent Protein 29. Chapter 21: Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters 30. Chapter 22: Measuring Protein Degradation with Green Fluorescent Protein 31. Chapter 23: Studying Nuclear Receptors with Green Fluorescent Protein Fusions 32. Chapter 24: Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein Conjugates 33. Chapter 25: Fluorescent Proteins in Single- and Multicolor Flow Cytometry 34. Chapter 26: Jellyfish Green Fluorescent Protein 35. Chapter 27: Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis Oocytes 36. Chapter 28: Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living Cells 37. Part IV: Laser Capture Microdissection 38. Chapter 29: Laser Capture Microdissection and Its Applications in Genomics and Proteomics 39. Chapter 30: Going In Vivo with Laser Microdissection 40. Chapter 31: Fluorescence In Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections 41. Index
As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.
1. Cover 2. Title Page 3. Copyright 4. Contributors 5. Preface 6. Part I: Practical Considerations and Equipment 7. Chapter 1: Theoretical Basis of Confocal Microscopy 8. Chapter 2: Practical Considerations in Acquiring Biological Signals from Confocal Microscope 9. Chapter 3: Equipment for Mass Storage and Processing of Data 10. Chapter 4: Antifading Agents for Confocal Fluorescence Microscopy 11. Chapter 5: Mounting Techniques for Confocal Microscopy 12. Chapter 6: Preparation of Whole Mounts and Thick Sections for Confocal Microscopy 13. Chapter 7: Use of Confocal Microscopy to Investigate Cell Structure and Function 14. Chapter 8: Combining Laser Scanning Confocal Microscopy and Electron Microscopy 15. Part II: Functional Approaches 16. Chapter 9: Volume Measurements in Confocal Microscopy 17. Chapter 10: Quantitation of Phagocytosis by Confocal Microscopy 18. Chapter 11: Receptor–Ligand Internalization 19. Chapter 12: Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy 20. Chapter 13: Trafficking of the Androgen Receptor 21. Chapter 14: Confocal Microscopy 22. Chapter 15: Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues; Functional Metabolic Imaging of Human Skin In Vivo 23. Chapter 16: Video-Rate, Scanning Slit Confocal Microscopy of Living Human Cornea In Vivo 24. Chapter 17: In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) Method 25. Chapter 18: Identification of Viral Infection by Confocal Microscopy 26. Chapter 19: Membrane Trafficking 27. Part III: Green Fluorescent Protein 28. Chapter 20: Monitoring of Protein Secretion with Green Fluorescent Protein 29. Chapter 21: Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters 30. Chapter 22: Measuring Protein Degradation with Green Fluorescent Protein 31. Chapter 23: Studying Nuclear Receptors with Green Fluorescent Protein Fusions 32. Chapter 24: Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein Conjugates 33. Chapter 25: Fluorescent Proteins in Single- and Multicolor Flow Cytometry 34. Chapter 26: Jellyfish Green Fluorescent Protein 35. Chapter 27: Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis Oocytes 36. Chapter 28: Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living Cells 37. Part IV: Laser Capture Microdissection 38. Chapter 29: Laser Capture Microdissection and Its Applications in Genomics and Proteomics 39. Chapter 30: Going In Vivo with Laser Microdissection 40. Chapter 31: Fluorescence In Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections 41. Index
