The Zebrafish: Genetics, Genomics, and Transcriptomics, Fourth Edition, is the latest volume in the Methods in Cell Biology series that looks at methods for the analysis of genetics, genomics, and transcriptomics of Zebrafish.
Chapters cover such topics as gene-trap mutagenesis, genetic Screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, and developmental epigenetics and the zebrafish interactome.
1. Cover image - p. i 2. Title page - p. i 3. Table of Contents - p. iii 4. Series Editors - p. ii 5. Copyright - p. iv 6. Dedication - p. v 7. Contributors - p. xvii 8. Preface - p. xxv 9. Part 1: Forward and Reverse Genetics - p. xxv 10. Chapter 1. Multiplex conditional mutagenesis in zebrafish using the CRISPR/Cas system - p. 3 11. Chapter 2. Tol2-mediated transgenesis, gene trapping, enhancer trapping, and Gal4-UAS system - p. 19 12. Chapter 3. Genetic screens for mutations affecting adult traits and parental-effect genes - p. 39 13. Chapter 4. Targeted candidate gene screens using CRISPR/Cas9 technology - p. 89 14. Chapter 5. TALEN- and CRISPR-enhanced DNA homologous recombination for gene editing in zebrafish - p. 107 15. Chapter 6. Precise genome editing by homologous recombination - p. 121 16. Chapter 7. The zebrafish genome editing toolkit - p. 149 17. Chapter 8. Clonal analysis of gene loss of function and tissue-specific gene deletion in zebrafish via CRISPR/Cas9 technology - p. 171 18. Chapter 9. Tissue-specific gene targeting using CRISPR/Cas9 - p. 189 19. Part 2: Transgenesis and Functional Genomics - p. 202 20. Chapter 10. Transcriptional regulation using the Q system in transgenic zebrafish - p. 205 21. Chapter 11. Contemporary zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments - p. 219 22. Chapter 12. Method for somatic cell nuclear transfer in zebrafish - p. 245 23. Chapter 13. Experimental approaches to studying the nature and impact of splicing variation in zebrafish - p. 259 24. Chapter 14. PICCORO: A technique for manipulating the activity of transcription factors with blue light - p. 289 25. Part 3: Transcriptomics - p. 295 26. Chapter 15. Tomo-seq: A method to obtain genome-wide expression data with spatial resolution - p. 299 27. Chapter 16. Cell type-specific transcriptomic analysis by thiouracil tagging in zebrafish - p. 309 28. Chapter 17. RT-qPCR gene expression analysis in zebrafish: Preanalytical precautions and use of expressed repetitive elements for normalization - p. 329 29. Part 4: Epigenetics - p. 342 30. Chapter 18. Genome-wide DNA methylation profiling in zebrafish - p. 345 31. Chapter 19. Histone modifications in zebrafish development - p. 361 32. Chapter 20. Chromatin immunoprecipitation and an open chromatin assay in zebrafish erythrocytes - p. 387 33. Chapter 21. Assay for transposase-accessible chromatin and circularized chromosome conformation capture, two methods to explore the regulatory landscapes of genes in zebrafish - p. 413 34. Chapter 22. Epigenetic regulation of hematopoietic stem cell development - p. 431 35. Part 5: Zebrafish Infrastructure - p. 448 36. Chapter 23. A scientist's guide for submitting data to ZFIN - p. 451 37. Chapter 24. New frontiers for zebrafish management - p. 483 38. Chapter 25. Aquaculture, husbandry, and shipping at the Zebrafish International Resource Center - p. 509 39. Chapter 26. Health monitoring and disease prevention at the Zebrafish International Resource Center - p. 535 40. Volumes in Series - p. 553 41. Index - p. 567
The Zebrafish: Genetics, Genomics, and Transcriptomics, Fourth Edition, is the latest volume in the Methods in Cell Biology series that looks at methods for the analysis of genetics, genomics, and transcriptomics of Zebrafish.
Chapters cover such topics as gene-trap mutagenesis, genetic Screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, and developmental epigenetics and the zebrafish interactome.
1. Cover image - p. i 2. Title page - p. i 3. Table of Contents - p. iii 4. Series Editors - p. ii 5. Copyright - p. iv 6. Dedication - p. v 7. Contributors - p. xvii 8. Preface - p. xxv 9. Part 1: Forward and Reverse Genetics - p. xxv 10. Chapter 1. Multiplex conditional mutagenesis in zebrafish using the CRISPR/Cas system - p. 3 11. Chapter 2. Tol2-mediated transgenesis, gene trapping, enhancer trapping, and Gal4-UAS system - p. 19 12. Chapter 3. Genetic screens for mutations affecting adult traits and parental-effect genes - p. 39 13. Chapter 4. Targeted candidate gene screens using CRISPR/Cas9 technology - p. 89 14. Chapter 5. TALEN- and CRISPR-enhanced DNA homologous recombination for gene editing in zebrafish - p. 107 15. Chapter 6. Precise genome editing by homologous recombination - p. 121 16. Chapter 7. The zebrafish genome editing toolkit - p. 149 17. Chapter 8. Clonal analysis of gene loss of function and tissue-specific gene deletion in zebrafish via CRISPR/Cas9 technology - p. 171 18. Chapter 9. Tissue-specific gene targeting using CRISPR/Cas9 - p. 189 19. Part 2: Transgenesis and Functional Genomics - p. 202 20. Chapter 10. Transcriptional regulation using the Q system in transgenic zebrafish - p. 205 21. Chapter 11. Contemporary zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments - p. 219 22. Chapter 12. Method for somatic cell nuclear transfer in zebrafish - p. 245 23. Chapter 13. Experimental approaches to studying the nature and impact of splicing variation in zebrafish - p. 259 24. Chapter 14. PICCORO: A technique for manipulating the activity of transcription factors with blue light - p. 289 25. Part 3: Transcriptomics - p. 295 26. Chapter 15. Tomo-seq: A method to obtain genome-wide expression data with spatial resolution - p. 299 27. Chapter 16. Cell type-specific transcriptomic analysis by thiouracil tagging in zebrafish - p. 309 28. Chapter 17. RT-qPCR gene expression analysis in zebrafish: Preanalytical precautions and use of expressed repetitive elements for normalization - p. 329 29. Part 4: Epigenetics - p. 342 30. Chapter 18. Genome-wide DNA methylation profiling in zebrafish - p. 345 31. Chapter 19. Histone modifications in zebrafish development - p. 361 32. Chapter 20. Chromatin immunoprecipitation and an open chromatin assay in zebrafish erythrocytes - p. 387 33. Chapter 21. Assay for transposase-accessible chromatin and circularized chromosome conformation capture, two methods to explore the regulatory landscapes of genes in zebrafish - p. 413 34. Chapter 22. Epigenetic regulation of hematopoietic stem cell development - p. 431 35. Part 5: Zebrafish Infrastructure - p. 448 36. Chapter 23. A scientist's guide for submitting data to ZFIN - p. 451 37. Chapter 24. New frontiers for zebrafish management - p. 483 38. Chapter 25. Aquaculture, husbandry, and shipping at the Zebrafish International Resource Center - p. 509 39. Chapter 26. Health monitoring and disease prevention at the Zebrafish International Resource Center - p. 535 40. Volumes in Series - p. 553 41. Index - p. 567
