tRNA-derived RNAs series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Purification of endogenous tDRs by hybridization-based pulldown - p. 2 9. Chapter Two: Northern blotting for human pre-tRNA and tRNA-derived RNAs - p. 16 10. Chapter Three: A general framework to analyze potential roles of tDRs in mammalian protein synthesis - p. 30 11. Chapter Four: tRNA-derived RNAs that form tetramolecular assemblies - p. 48 12. Chapter Five: Ribozyme-mediated expression of tRNA-derived small RNAs in bacteria - p. 66 13. Chapter Six: Ribosome-associated tDRs in yeast - p. 86 14. Chapter Seven: Analyzing, visualizing, and annotating tRNA-derived RNAs using tRAX and tDRnamer - p. 104 15. Chapter Eight: cP-RNA-seq for tRNA half sequencing - p. 136 16. Chapter Nine: TaqMan RT-qPCR for tRNA half quantification - p. 156 17. Chapter Ten: Discrimination between vesicular and nonvesicular extracellular tRNAs and their fragments - p. 172 18. Chapter Eleven: Methods for purification and characterization of nicked tRNAs - p. 188 19. Chapter Twelve: In vitro functional analysis of plant tDRs - p. 204 20. Chapter Thirteen: TGIRT-seq to profile tRNA-derived RNAs and associated RNA modifications - p. 224 21. Chapter Fourteen: A general framework to over-express tRNA-derived fragments from their parental tRNAs in mammalian cells - p. 243 22. Chapter Fifteen: Functional characterization of tRNA-derived small RNAs in stem cells - p. 262 23. Chapter Sixteen: Detection of mitochondrial tDRs in killifish embryos and other non-model organisms - p. 284 24. Chapter Seventeen: Bioinformatics of simultaneous, quantitative measurements of full-length tRNA and tRNA fragments by MSR sequencing - p. 314 25. Chapter Eighteen: Self-quenched tRNA reporters for imaging tRNA-derived RNA biogenesis - p. 326 26. Chapter Nineteen: Functional analysis of tRNA-derived small translational regulation - p. 338 27. Chapter Twenty: Determining small RNA-interacting proteomes using endogenously modified tRNA-derived RNAs - p. 355 28. Chapter Twenty One: Assay for ribosome stimulation of angiogenin nuclease activity - p. 384
tRNA-derived RNAs series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Purification of endogenous tDRs by hybridization-based pulldown - p. 2 9. Chapter Two: Northern blotting for human pre-tRNA and tRNA-derived RNAs - p. 16 10. Chapter Three: A general framework to analyze potential roles of tDRs in mammalian protein synthesis - p. 30 11. Chapter Four: tRNA-derived RNAs that form tetramolecular assemblies - p. 48 12. Chapter Five: Ribozyme-mediated expression of tRNA-derived small RNAs in bacteria - p. 66 13. Chapter Six: Ribosome-associated tDRs in yeast - p. 86 14. Chapter Seven: Analyzing, visualizing, and annotating tRNA-derived RNAs using tRAX and tDRnamer - p. 104 15. Chapter Eight: cP-RNA-seq for tRNA half sequencing - p. 136 16. Chapter Nine: TaqMan RT-qPCR for tRNA half quantification - p. 156 17. Chapter Ten: Discrimination between vesicular and nonvesicular extracellular tRNAs and their fragments - p. 172 18. Chapter Eleven: Methods for purification and characterization of nicked tRNAs - p. 188 19. Chapter Twelve: In vitro functional analysis of plant tDRs - p. 204 20. Chapter Thirteen: TGIRT-seq to profile tRNA-derived RNAs and associated RNA modifications - p. 224 21. Chapter Fourteen: A general framework to over-express tRNA-derived fragments from their parental tRNAs in mammalian cells - p. 243 22. Chapter Fifteen: Functional characterization of tRNA-derived small RNAs in stem cells - p. 262 23. Chapter Sixteen: Detection of mitochondrial tDRs in killifish embryos and other non-model organisms - p. 284 24. Chapter Seventeen: Bioinformatics of simultaneous, quantitative measurements of full-length tRNA and tRNA fragments by MSR sequencing - p. 314 25. Chapter Eighteen: Self-quenched tRNA reporters for imaging tRNA-derived RNA biogenesis - p. 326 26. Chapter Nineteen: Functional analysis of tRNA-derived small translational regulation - p. 338 27. Chapter Twenty: Determining small RNA-interacting proteomes using endogenously modified tRNA-derived RNAs - p. 355 28. Chapter Twenty One: Assay for ribosome stimulation of angiogenin nuclease activity - p. 384
