Tumor Immunology and Immunotherapy – Cellular Methods Part A, Volume 631, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters include Detection of intracellular cytokine production by T cells with flow cytometry, High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers, In vitro assays for effector T cell functions and activity of immunostimulatory antibodies, Ex vivo energetic profiling of tumor cells and T cells from mouse models and human samples, A cytofluorimetric assay to evaluate T cell polyfunctionality, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xv 6. Preface: More than two decades of modern tumor immunology - p. page_xv 7. Chapter One: Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry - p. page_xlii 8. Chapter Two: High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers - p. page_20 9. Chapter Three: In vitro assays for effector T cell functions and activity of immunomodulatory antibodies - p. page_42 10. Chapter Four: A cytofluorimetric assay to evaluate T cell polyfunctionality - p. page_59 11. Chapter Five: Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells - p. page_76 12. Chapter Six: Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma models - p. page_90 13. Chapter Seven: Methods to edit T cells for cancer immunotherapy - p. page_106 14. Chapter Eight: Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer - p. page_135 15. Chapter Nine: Reverse immunology: From peptide sequence to tumor-killing human T-cell clones - p. page_158 16. Chapter Ten: Generation of TCR-engineered reference cell samples to control T-cell assay performance - p. page_194 17. Chapter Eleven: In vitro expansion of Vγ9Vδ2 T cells for immunotherapy - p. page_221 18. Chapter Twelve: CFSE dilution to study human T and NK cell proliferation in vitro - p. page_237 19. Chapter Thirteen: Rapid isolation and enrichment of mouse NK cells for experimental purposes - p. page_255 20. Chapter Fourteen: Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood - p. page_275 21. Chapter Fifteen: Two-dimensional dynamic evaluation of natural killer cell-mediated lysis of adherent target cells - p. page_287 22. Chapter Sixteen: Rapid isolation of mouse ILCs from murine intestinal tissues - p. page_304 23. Chapter Seventeen: Detecting and analyzing murine innate lymphoid cells - p. page_327 24. Chapter Eighteen: A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells - p. page_342 25. Chapter Nineteen: Applications of microfluidic devices in advancing NK-cell migration studies - p. page_355 26. Chapter Twenty: Complementary approaches to study NKT cells in cancer - p. page_370 27. Chapter Twenty-One: Assessment of IFN-γ and granzyme-B production by in “sitro” technology - p. page_389 28. Chapter Twenty-Two: Assessment of IFNγ responsiveness in patient-derived xenografts - p. page_414 29. Chapter Twenty-Three: Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro - p. page_427 30. Chapter Twenty-Four: Analysis of cancer cell-intrinsic immune regulation in response to CD8 + T cell attack - p. page_441 31. Chapter Twenty-Five: A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicity - p. page_466 32. Chapter Twenty-Six: Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay - p. page_482 33. Chapter Twenty-Seven: 51Cr-release to monitor NK cell cytotoxicity - p. page_495
Tumor Immunology and Immunotherapy – Cellular Methods Part A, Volume 631, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters include Detection of intracellular cytokine production by T cells with flow cytometry, High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers, In vitro assays for effector T cell functions and activity of immunostimulatory antibodies, Ex vivo energetic profiling of tumor cells and T cells from mouse models and human samples, A cytofluorimetric assay to evaluate T cell polyfunctionality, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xv 6. Preface: More than two decades of modern tumor immunology - p. page_xv 7. Chapter One: Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry - p. page_xlii 8. Chapter Two: High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers - p. page_20 9. Chapter Three: In vitro assays for effector T cell functions and activity of immunomodulatory antibodies - p. page_42 10. Chapter Four: A cytofluorimetric assay to evaluate T cell polyfunctionality - p. page_59 11. Chapter Five: Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells - p. page_76 12. Chapter Six: Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma models - p. page_90 13. Chapter Seven: Methods to edit T cells for cancer immunotherapy - p. page_106 14. Chapter Eight: Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer - p. page_135 15. Chapter Nine: Reverse immunology: From peptide sequence to tumor-killing human T-cell clones - p. page_158 16. Chapter Ten: Generation of TCR-engineered reference cell samples to control T-cell assay performance - p. page_194 17. Chapter Eleven: In vitro expansion of Vγ9Vδ2 T cells for immunotherapy - p. page_221 18. Chapter Twelve: CFSE dilution to study human T and NK cell proliferation in vitro - p. page_237 19. Chapter Thirteen: Rapid isolation and enrichment of mouse NK cells for experimental purposes - p. page_255 20. Chapter Fourteen: Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood - p. page_275 21. Chapter Fifteen: Two-dimensional dynamic evaluation of natural killer cell-mediated lysis of adherent target cells - p. page_287 22. Chapter Sixteen: Rapid isolation of mouse ILCs from murine intestinal tissues - p. page_304 23. Chapter Seventeen: Detecting and analyzing murine innate lymphoid cells - p. page_327 24. Chapter Eighteen: A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells - p. page_342 25. Chapter Nineteen: Applications of microfluidic devices in advancing NK-cell migration studies - p. page_355 26. Chapter Twenty: Complementary approaches to study NKT cells in cancer - p. page_370 27. Chapter Twenty-One: Assessment of IFN-γ and granzyme-B production by in “sitro” technology - p. page_389 28. Chapter Twenty-Two: Assessment of IFNγ responsiveness in patient-derived xenografts - p. page_414 29. Chapter Twenty-Three: Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro - p. page_427 30. Chapter Twenty-Four: Analysis of cancer cell-intrinsic immune regulation in response to CD8 + T cell attack - p. page_441 31. Chapter Twenty-Five: A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicity - p. page_466 32. Chapter Twenty-Six: Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay - p. page_482 33. Chapter Twenty-Seven: 51Cr-release to monitor NK cell cytotoxicity - p. page_495
