Tumor Immunology and Immunotherapy – Cellular Methods Part B, Volume 632, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered include Quantitation of calreticulin exposure associated with immunogenic cell death, Side-by-side comparisons of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death, Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry, Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies, Methods to assess DC-dependent priming of T cell responses by dying cells, and more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xv 6. Preface: More than two decades of modern tumor immunology - p. page_xl 7. Chapter One: Quantitation of calreticulin exposure associated with immunogenic cell death - p. page_xlii 8. Chapter Two: Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death - p. page_13 9. Chapter Three: Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry - p. page_25 10. Chapter Four: Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies - p. page_37 11. Chapter Five: Methods to assess DC-dependent priming of T cell responses by dying cells - p. page_54 12. Chapter Six: Isolation of mononuclear phagocytes from the mouse gut - p. page_65 13. Chapter Seven: Generation of mouse bone marrow-derived macrophages using tumor coculture assays to mimic the tumor microenvironment - p. page_90 14. Chapter Eight: Methods for macrophage differentiation and in vitro generation of human tumor associated-like macrophages - p. page_111 15. Chapter Nine: In vitro assessment of cancer cell-induced polarization of macrophages - p. page_131 16. Chapter Ten: Protocol to assess the suppression of T-cell proliferation by human MDSC - p. page_154 17. Chapter Eleven: Detection and functional evaluation of arginase-1 isolated from human PMNs and murine MDSC - p. page_192 18. Chapter Twelve: Functional assay to assess T-cell inhibitory properties of myeloid derived suppressor cells (MDSCs) isolated from the tumor microenvironment of murine glioma models - p. page_213 19. Chapter Thirteen: FACS isolation and analysis of human circulating and tumor neutrophils - p. page_228 20. Chapter Fourteen: Flow cytometry-based isolation of tumor-associated regulatory T cells and assessment of their suppressive potential - p. page_257 21. Chapter Fifteen: Assessment of lipid load in tumor-infiltrating Tregs by flow cytometry - p. page_281 22. Chapter Sixteen: Preparation of single cells from tumors for single-cell RNA sequencing - p. page_294 23. Chapter Seventeen: Characterization of the tumor immune infiltrate by multiparametric flow cytometry and unbiased high-dimensional data analysis - p. page_308 24. Chapter Eighteen: Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF) - p. page_337 25. Chapter Nineteen: Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system - p. page_368 26. Chapter Twenty: Analysis of the immune status from peripheral whole blood with a single-tube multicolor flow cytometry assay - p. page_388 27. Chapter Twenty-One: Ex vivo assessment of in vivo DC-targeted antibodies in pre-clinical models - p. page_415 28. Chapter Twenty-Two: Flow cytometry-based assessment of direct-targeting anti-cancer antibody immune effector functions - p. page_430 29. Chapter Twenty-Three: Enzyme-linked immunospot assay to monitor antigen-specific cellular immune responses in mouse tumor models - p. page_456 30. Chapter Twenty-Four: High-throughput analysis of cell-cell crosstalk in ad hoc designed microfluidic chips for oncoimmunology applications - p. page_477 31. Chapter Twenty-Five: Generation of affinity ranged antigen-expressing tumor cell lines - p. page_502 32. Chapter Twenty-Six: Recombinant fusion proteins for targeting dendritic cell subsets in therapeutic cancer vaccine - p. page_519
Tumor Immunology and Immunotherapy – Cellular Methods Part B, Volume 632, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered include Quantitation of calreticulin exposure associated with immunogenic cell death, Side-by-side comparisons of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death, Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry, Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies, Methods to assess DC-dependent priming of T cell responses by dying cells, and more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xv 6. Preface: More than two decades of modern tumor immunology - p. page_xl 7. Chapter One: Quantitation of calreticulin exposure associated with immunogenic cell death - p. page_xlii 8. Chapter Two: Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death - p. page_13 9. Chapter Three: Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry - p. page_25 10. Chapter Four: Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies - p. page_37 11. Chapter Five: Methods to assess DC-dependent priming of T cell responses by dying cells - p. page_54 12. Chapter Six: Isolation of mononuclear phagocytes from the mouse gut - p. page_65 13. Chapter Seven: Generation of mouse bone marrow-derived macrophages using tumor coculture assays to mimic the tumor microenvironment - p. page_90 14. Chapter Eight: Methods for macrophage differentiation and in vitro generation of human tumor associated-like macrophages - p. page_111 15. Chapter Nine: In vitro assessment of cancer cell-induced polarization of macrophages - p. page_131 16. Chapter Ten: Protocol to assess the suppression of T-cell proliferation by human MDSC - p. page_154 17. Chapter Eleven: Detection and functional evaluation of arginase-1 isolated from human PMNs and murine MDSC - p. page_192 18. Chapter Twelve: Functional assay to assess T-cell inhibitory properties of myeloid derived suppressor cells (MDSCs) isolated from the tumor microenvironment of murine glioma models - p. page_213 19. Chapter Thirteen: FACS isolation and analysis of human circulating and tumor neutrophils - p. page_228 20. Chapter Fourteen: Flow cytometry-based isolation of tumor-associated regulatory T cells and assessment of their suppressive potential - p. page_257 21. Chapter Fifteen: Assessment of lipid load in tumor-infiltrating Tregs by flow cytometry - p. page_281 22. Chapter Sixteen: Preparation of single cells from tumors for single-cell RNA sequencing - p. page_294 23. Chapter Seventeen: Characterization of the tumor immune infiltrate by multiparametric flow cytometry and unbiased high-dimensional data analysis - p. page_308 24. Chapter Eighteen: Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF) - p. page_337 25. Chapter Nineteen: Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system - p. page_368 26. Chapter Twenty: Analysis of the immune status from peripheral whole blood with a single-tube multicolor flow cytometry assay - p. page_388 27. Chapter Twenty-One: Ex vivo assessment of in vivo DC-targeted antibodies in pre-clinical models - p. page_415 28. Chapter Twenty-Two: Flow cytometry-based assessment of direct-targeting anti-cancer antibody immune effector functions - p. page_430 29. Chapter Twenty-Three: Enzyme-linked immunospot assay to monitor antigen-specific cellular immune responses in mouse tumor models - p. page_456 30. Chapter Twenty-Four: High-throughput analysis of cell-cell crosstalk in ad hoc designed microfluidic chips for oncoimmunology applications - p. page_477 31. Chapter Twenty-Five: Generation of affinity ranged antigen-expressing tumor cell lines - p. page_502 32. Chapter Twenty-Six: Recombinant fusion proteins for targeting dendritic cell subsets in therapeutic cancer vaccine - p. page_519
