Tumor Immunology and Immunotherapy – Molecular Methods, Volume 629, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this release include Droplet digital PCR for measuring circulating tumor-derived DNA, Detection and quantification of cytosolic DNA, Methods to detect endogenous dsRNA induction and recognition, Quantification of eIF2alpha phosphorylation during immunogenic cell death, Assessment of annexin A1 release during immunogenic cell death, Luciferase-assisted detection of extracellular ATP in the course of ICD, The P2X7 receptor: structure and function, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface: More than two decades of modern tumor immunology - p. xxi 7. Chapter One: Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies - p. pgxl 8. Chapter Two: Detection and quantification of cytosolic DNA - p. 15 9. Chapter Three: Methods to detect endogenous dsRNA induction and recognition - p. 33 10. Chapter Four: Quantification of eIF2alpha phosphorylation during immunogenic cell death - p. 51 11. Chapter Five: Assessment of annexin A1 release during immunogenic cell death - p. 69 12. Chapter Six: Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells - p. 79 13. Chapter Seven: Quinacrine-mediated detection of intracellular ATP - p. 102 14. Chapter Eight: Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells - p. 113 15. Chapter Nine: LEGENDplex™: Bead-assisted multiplex cytokine profiling by flow cytometry - p. 150 16. Chapter Ten: Methods for measuring HMGB1 release during immunogenic cell death - p. 176 17. Chapter Eleven: Different measures of HMGB1 location in cancer immunology - p. 193 18. Chapter Twelve: Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues - p. 217 19. Chapter Thirteen: Quantification of IDO1 enzyme activity in normal and malignant tissues - p. 233 20. Chapter Fourteen: Enzyme activity of circulating CD73 in human serum - p. 256 21. Chapter Fifteen: Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays - p. 267 22. Chapter Sixteen: Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis - p. 288 23. Chapter Seventeen: Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization - p. 306 24. Chapter Eighteen: In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1 - p. 360 25. Chapter Nineteen: Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies - p. 381 26. Chapter Twenty: High-throughput discovery of cancer-targeting TCRs - p. 399 27. Chapter Twenty-One: TCR validation toward gene therapy for cancer - p. 417 28. Chapter Twenty-Two: Analysis of TCR β CDR3 sequencing data for tracking anti-tumor immunity - p. 441 29. Chapter Twenty-Three: Quantitative analysis of the T cell receptor repertoire - p. 464 30. Chapter Twenty-Four: CRATES: A one-step assembly method for Class 2 CRISPR arrays - p. 492
Tumor Immunology and Immunotherapy – Molecular Methods, Volume 629, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this release include Droplet digital PCR for measuring circulating tumor-derived DNA, Detection and quantification of cytosolic DNA, Methods to detect endogenous dsRNA induction and recognition, Quantification of eIF2alpha phosphorylation during immunogenic cell death, Assessment of annexin A1 release during immunogenic cell death, Luciferase-assisted detection of extracellular ATP in the course of ICD, The P2X7 receptor: structure and function, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface: More than two decades of modern tumor immunology - p. xxi 7. Chapter One: Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies - p. pgxl 8. Chapter Two: Detection and quantification of cytosolic DNA - p. 15 9. Chapter Three: Methods to detect endogenous dsRNA induction and recognition - p. 33 10. Chapter Four: Quantification of eIF2alpha phosphorylation during immunogenic cell death - p. 51 11. Chapter Five: Assessment of annexin A1 release during immunogenic cell death - p. 69 12. Chapter Six: Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells - p. 79 13. Chapter Seven: Quinacrine-mediated detection of intracellular ATP - p. 102 14. Chapter Eight: Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells - p. 113 15. Chapter Nine: LEGENDplex™: Bead-assisted multiplex cytokine profiling by flow cytometry - p. 150 16. Chapter Ten: Methods for measuring HMGB1 release during immunogenic cell death - p. 176 17. Chapter Eleven: Different measures of HMGB1 location in cancer immunology - p. 193 18. Chapter Twelve: Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues - p. 217 19. Chapter Thirteen: Quantification of IDO1 enzyme activity in normal and malignant tissues - p. 233 20. Chapter Fourteen: Enzyme activity of circulating CD73 in human serum - p. 256 21. Chapter Fifteen: Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays - p. 267 22. Chapter Sixteen: Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis - p. 288 23. Chapter Seventeen: Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization - p. 306 24. Chapter Eighteen: In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1 - p. 360 25. Chapter Nineteen: Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies - p. 381 26. Chapter Twenty: High-throughput discovery of cancer-targeting TCRs - p. 399 27. Chapter Twenty-One: TCR validation toward gene therapy for cancer - p. 417 28. Chapter Twenty-Two: Analysis of TCR β CDR3 sequencing data for tracking anti-tumor immunity - p. 441 29. Chapter Twenty-Three: Quantitative analysis of the T cell receptor repertoire - p. 464 30. Chapter Twenty-Four: CRATES: A one-step assembly method for Class 2 CRISPR arrays - p. 492
