ADARs, Volume 710 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Updates in this new release include Yeast as a discovery tool for hyperactive ADARs, X-ray crystallography of ADAR-RNA complexes, Enrichment capture enhances the ability to detect A-to-I editing, Editing specificity of ADAR isoforms, Novel chemical tools for detection methods of A to I sites, En Masse Evaluation of RNA Guides (EMERGe) for ADARs, Mouse models for defining physiological functions of ADARs, Nanopore sequencing to detect A-to-I editing sites, CellREADR: An ADAR-based RNA sensor, and more.
Additional sections cover Site-directed RNA editing with MS2-ADAR systems, Sequencing and Bioinformatic Tools for Accurate Assessment of A to I editing in Complete Transcriptomes, Aptazyme-directed A to I RNA editing, and Obstacles in quantifying A-to-I editing by Sanger Sequencing.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Preface - p. xvii 9. Chapter One: Leveraging Saccharomyces cerevisiae for ADAR research: From high-yield purification to high-throughput screening and therapeutic applications - p. 1 10. Chapter Two: Structural analysis of human ADAR2-RNA complexes by X-ray crystallography - p. 19 11. Chapter Three: A probe-based capture enrichment method for detection of A-to-I editing in low abundance transcripts - p. 55 12. Chapter Four: Editing specificity of ADAR isoforms - p. 77 13. Chapter Five: EndoVIA for quantifying A-to-I editing and mapping the subcellular localization of edited transcripts - p. 99 14. Chapter Six: En masse evaluation of RNA guides (EMERGe) for ADARs - p. 131 15. Chapter Seven: Mouse models for understanding physiological functions of ADARs - p. 153 16. Chapter Eight: Nanopore sequencing to detect A-to-I editing sites - p. 187 17. Chapter Nine: CellREADR: An ADAR-based RNA sensor-actuator device - p. 207 18. Chapter Ten: Restoration of G to A mutated transcripts using the MS2-ADAR1 system - p. 229 19. Chapter Eleven: Bioinformatic approaches for accurate assessment of A-to-I editing in complete transcriptomes - p. 241 20. Chapter Twelve: Aptazyme-directed A-to-I RNA editing - p. 267 21. Chapter Thirteen: Obstacles in quantifying A-to-I RNA editing by Sanger sequencing - p. 285
ADARs, Volume 710 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Updates in this new release include Yeast as a discovery tool for hyperactive ADARs, X-ray crystallography of ADAR-RNA complexes, Enrichment capture enhances the ability to detect A-to-I editing, Editing specificity of ADAR isoforms, Novel chemical tools for detection methods of A to I sites, En Masse Evaluation of RNA Guides (EMERGe) for ADARs, Mouse models for defining physiological functions of ADARs, Nanopore sequencing to detect A-to-I editing sites, CellREADR: An ADAR-based RNA sensor, and more.
Additional sections cover Site-directed RNA editing with MS2-ADAR systems, Sequencing and Bioinformatic Tools for Accurate Assessment of A to I editing in Complete Transcriptomes, Aptazyme-directed A to I RNA editing, and Obstacles in quantifying A-to-I editing by Sanger Sequencing.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Preface - p. xvii 9. Chapter One: Leveraging Saccharomyces cerevisiae for ADAR research: From high-yield purification to high-throughput screening and therapeutic applications - p. 1 10. Chapter Two: Structural analysis of human ADAR2-RNA complexes by X-ray crystallography - p. 19 11. Chapter Three: A probe-based capture enrichment method for detection of A-to-I editing in low abundance transcripts - p. 55 12. Chapter Four: Editing specificity of ADAR isoforms - p. 77 13. Chapter Five: EndoVIA for quantifying A-to-I editing and mapping the subcellular localization of edited transcripts - p. 99 14. Chapter Six: En masse evaluation of RNA guides (EMERGe) for ADARs - p. 131 15. Chapter Seven: Mouse models for understanding physiological functions of ADARs - p. 153 16. Chapter Eight: Nanopore sequencing to detect A-to-I editing sites - p. 187 17. Chapter Nine: CellREADR: An ADAR-based RNA sensor-actuator device - p. 207 18. Chapter Ten: Restoration of G to A mutated transcripts using the MS2-ADAR1 system - p. 229 19. Chapter Eleven: Bioinformatic approaches for accurate assessment of A-to-I editing in complete transcriptomes - p. 241 20. Chapter Twelve: Aptazyme-directed A-to-I RNA editing - p. 267 21. Chapter Thirteen: Obstacles in quantifying A-to-I RNA editing by Sanger sequencing - p. 285
