Advances in Biomolecular EPR, Volume 666 in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics including Magnetic Resonance Characterization of Physiologically Important Metal Ion Binding Sites in the Prion and Related Proteins, The catalytic role of metal-radical/protein-based radicals in heme enzymes, Rigid Cu2+-based spin labels for the study of higher-order DNA G-quadruplex structures, Orthogonal spin labeling and membrane proteins: increasing the information content and going towards in cell applications, Spectroscopic investigation of mono- and di-Mn-containing centers in biochemistry with an emphasis on application of paramagnetic resonance techniques, and more.
Additional chapters cover In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance, an Update, EPR contributions to understanding molybdenum-containing enzymes, EPR spectroscopy of Type I reaction centers, Characterization of a substrate-derived radical in the NosN reaction during the biosynthesis of nosiheptide, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter One: Advances in rapid scan EPR spectroscopy - p. 1 8. Chapter Two: Resolution and characterization of confinement- and temperature-dependent dynamics in solvent phases that surround proteins in frozen aqueous solution by using spin-probe EPR spectroscopy - p. 25 9. Chapter Three: Site directed spin labeling to elucidating the mechanism of the cyanobacterial circadian clock - p. 59 10. Chapter Four: Orthogonal spin labeling and pulsed dipolar spectroscopy for protein studies - p. 79 11. Chapter Five: Tracking protein domain movements by EPR distance determination and multilateration - p. 121 12. Chapter Six: Integrative ensemble modeling of proteins and their complexes with distance distribution restraints - p. 145 13. Chapter Seven: Light-induced pulsed dipolar EPR spectroscopy for distance and orientation analysis - p. 171 14. Chapter Eight: Controlling and exploiting intrinsic unpaired electrons in metalloproteins - p. 233 15. Chapter Nine: EPR of copper centers in the prion protein - p. 297 16. Chapter Ten: Paramagnetic resonance investigation of mono- and di-manganese-containing systems in biochemistry - p. 315 17. Chapter Eleven: Application of EPR and related methods to molybdenum-containing enzymes - p. 380 18. Chapter Twelve: EPR of Type I photosynthetic reaction centers - p. 413 19. Chapter Thirteen: Experimental guidelines for trapping paramagnetic reaction intermediates in radical S-adenosylmethionine enzymes - p. 451 20. Chapter Fourteen: Using peptide substrate analogs to characterize a radical intermediate in NosN catalysis - p. 469
Advances in Biomolecular EPR, Volume 666 in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics including Magnetic Resonance Characterization of Physiologically Important Metal Ion Binding Sites in the Prion and Related Proteins, The catalytic role of metal-radical/protein-based radicals in heme enzymes, Rigid Cu2+-based spin labels for the study of higher-order DNA G-quadruplex structures, Orthogonal spin labeling and membrane proteins: increasing the information content and going towards in cell applications, Spectroscopic investigation of mono- and di-Mn-containing centers in biochemistry with an emphasis on application of paramagnetic resonance techniques, and more.
Additional chapters cover In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance, an Update, EPR contributions to understanding molybdenum-containing enzymes, EPR spectroscopy of Type I reaction centers, Characterization of a substrate-derived radical in the NosN reaction during the biosynthesis of nosiheptide, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter One: Advances in rapid scan EPR spectroscopy - p. 1 8. Chapter Two: Resolution and characterization of confinement- and temperature-dependent dynamics in solvent phases that surround proteins in frozen aqueous solution by using spin-probe EPR spectroscopy - p. 25 9. Chapter Three: Site directed spin labeling to elucidating the mechanism of the cyanobacterial circadian clock - p. 59 10. Chapter Four: Orthogonal spin labeling and pulsed dipolar spectroscopy for protein studies - p. 79 11. Chapter Five: Tracking protein domain movements by EPR distance determination and multilateration - p. 121 12. Chapter Six: Integrative ensemble modeling of proteins and their complexes with distance distribution restraints - p. 145 13. Chapter Seven: Light-induced pulsed dipolar EPR spectroscopy for distance and orientation analysis - p. 171 14. Chapter Eight: Controlling and exploiting intrinsic unpaired electrons in metalloproteins - p. 233 15. Chapter Nine: EPR of copper centers in the prion protein - p. 297 16. Chapter Ten: Paramagnetic resonance investigation of mono- and di-manganese-containing systems in biochemistry - p. 315 17. Chapter Eleven: Application of EPR and related methods to molybdenum-containing enzymes - p. 380 18. Chapter Twelve: EPR of Type I photosynthetic reaction centers - p. 413 19. Chapter Thirteen: Experimental guidelines for trapping paramagnetic reaction intermediates in radical S-adenosylmethionine enzymes - p. 451 20. Chapter Fourteen: Using peptide substrate analogs to characterize a radical intermediate in NosN catalysis - p. 469
