Antimicrobial Peptides: A Roadmap for Accelerating Discovery and Development covers the most important efforts of scientists and engineers worldwide to accelerate the process of discovery, production, and eventual market penetration of more potent antimicrobial peptides. These efforts have been fueled by emerging technologies such as artificial intelligence and data science, molecular and CFD simulations, easy-to-use process simulation packages, microfluidics, 3D-printing, among many others. Such technologies can now be implemented and scaled up quickly and at relatively low cost in low-budget production facilities, critical to moving to sustainable and marketable products worldwide. Discovering novel antimicrobial peptides rationally and cost-effectively has emerged as one of the significant challenges of modern biotechnology. Thus far, this process has been tedious and costly, resulting in molecules with activities far below those needed to address the current challenge of microbial resistance to antibiotics that takes the lives of thousands of people around the world every year. Finally, the book also highlights how multidisciplinary teams have assembled to address the challenges of manufacturing, biological testing, and clinical trials to finally reach complete translation.
1. Introduction—Antimicrobial peptides: From the bench to the bedside - p. xiii 2. Section A: Computational approaches - p. 1 3. Chapter 1 Bioinformatic methods for the design of antimicrobial peptides - p. 3 4. Chapter 2 Fundamentals of molecular dynamics for antimicrobial peptides’ discovery - p. 37 5. Chapter 3 Artificial intelligence for the discovery of antimicrobial peptides - p. 59 6. Chapter 4 Computational tools for handling large databases of biological relevance - p. 81 7. Chapter 5 Statistical analysis and data interpretation - p. 97 8. Section B: Experimental approaches - p. 119 9. Chapter 6 Chemical synthesis of peptides: Conventional and novel routes - p. 121 10. Chapter 7 Biological platforms for the production of antimicrobial peptides: Bacterial, yeast, and cell-free systems - p. 131 11. Chapter 8 Biological platforms for the production of antimicrobial peptides: Plants, insects, and mammalian cells systems - p. 165 12. Chapter 9 Classical and emerging approximations for the screening of antimicrobial peptide libraries - p. 195 13. Abstract - p. 195 14. 1 Introduction - p. 195 15. 2 Classical screening technologies for peptide libraries - p. 197 16. 3 Emerging technologies for peptide library screening - p. 202 17. 4 Approaches to monitoring screening in real time - p. 211 18. 5 Proven schemes for screening peptide libraries - p. 217 19. 6 Technical challenges in screening peptide libraries - p. 219 20. 7 Future perspectives - p. 220 21. 8 AI disclosure - p. 220 22. References - p. 220 23. Chapter 10 Scaling-up of biological production processes - p. 233 24. Chapter 11 Downstream processing for antimicrobial peptide production - p. 243 25. Chapter 12 Physicochemical and biochemical characterization of antimicrobial peptides - p. 259 26. Section C: Translational studies - p. 301 27. Chapter 13 Biological characterization of antimicrobial peptides: In vitro and in vivo studies - p. 303 28. Chapter 14 Preclinical and clinical studies - p. 343 29. Chapter 15 Formulation and product design - p. 377 30. Chapter 16 Packaging, long-term stability, and usability of antimicrobial peptide products - p. 393 31. Chapter 17 Synthetic peptides quality control and assurance - p. 405 32. Index - p. 417
Antimicrobial Peptides: A Roadmap for Accelerating Discovery and Development covers the most important efforts of scientists and engineers worldwide to accelerate the process of discovery, production, and eventual market penetration of more potent antimicrobial peptides. These efforts have been fueled by emerging technologies such as artificial intelligence and data science, molecular and CFD simulations, easy-to-use process simulation packages, microfluidics, 3D-printing, among many others. Such technologies can now be implemented and scaled up quickly and at relatively low cost in low-budget production facilities, critical to moving to sustainable and marketable products worldwide. Discovering novel antimicrobial peptides rationally and cost-effectively has emerged as one of the significant challenges of modern biotechnology. Thus far, this process has been tedious and costly, resulting in molecules with activities far below those needed to address the current challenge of microbial resistance to antibiotics that takes the lives of thousands of people around the world every year. Finally, the book also highlights how multidisciplinary teams have assembled to address the challenges of manufacturing, biological testing, and clinical trials to finally reach complete translation.
1. Introduction—Antimicrobial peptides: From the bench to the bedside - p. xiii 2. Section A: Computational approaches - p. 1 3. Chapter 1 Bioinformatic methods for the design of antimicrobial peptides - p. 3 4. Chapter 2 Fundamentals of molecular dynamics for antimicrobial peptides’ discovery - p. 37 5. Chapter 3 Artificial intelligence for the discovery of antimicrobial peptides - p. 59 6. Chapter 4 Computational tools for handling large databases of biological relevance - p. 81 7. Chapter 5 Statistical analysis and data interpretation - p. 97 8. Section B: Experimental approaches - p. 119 9. Chapter 6 Chemical synthesis of peptides: Conventional and novel routes - p. 121 10. Chapter 7 Biological platforms for the production of antimicrobial peptides: Bacterial, yeast, and cell-free systems - p. 131 11. Chapter 8 Biological platforms for the production of antimicrobial peptides: Plants, insects, and mammalian cells systems - p. 165 12. Chapter 9 Classical and emerging approximations for the screening of antimicrobial peptide libraries - p. 195 13. Abstract - p. 195 14. 1 Introduction - p. 195 15. 2 Classical screening technologies for peptide libraries - p. 197 16. 3 Emerging technologies for peptide library screening - p. 202 17. 4 Approaches to monitoring screening in real time - p. 211 18. 5 Proven schemes for screening peptide libraries - p. 217 19. 6 Technical challenges in screening peptide libraries - p. 219 20. 7 Future perspectives - p. 220 21. 8 AI disclosure - p. 220 22. References - p. 220 23. Chapter 10 Scaling-up of biological production processes - p. 233 24. Chapter 11 Downstream processing for antimicrobial peptide production - p. 243 25. Chapter 12 Physicochemical and biochemical characterization of antimicrobial peptides - p. 259 26. Section C: Translational studies - p. 301 27. Chapter 13 Biological characterization of antimicrobial peptides: In vitro and in vivo studies - p. 303 28. Chapter 14 Preclinical and clinical studies - p. 343 29. Chapter 15 Formulation and product design - p. 377 30. Chapter 16 Packaging, long-term stability, and usability of antimicrobial peptide products - p. 393 31. Chapter 17 Synthetic peptides quality control and assurance - p. 405 32. Index - p. 417
