Apobec Enzymes, Volume 713 in this series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Fluorescent shift assay for APOBECs RNA editing, Low Error Sequencing Methods to Detect APOBEC-mediated RNA editing: Circular RNAseq and Safe-Sequencing System, "Safe-Barcode" RNAseq assay for APOBECs RNA editing, DT40 cell system to characterize somatic hypermutation, CH12 cell system to assay AID activity on class switch recombination, Purification of Enzymatically Active APOBEC Proteins from an Insect Cell Expression System, and more.
Additional chapters cover Defining genome-wide mutagenic impact of APOBEC3 enzymes, APOBEC-induced mutational assay in yeast, Assays for APOBEC drug discovery, Biochemical assay for the identification of APOBECs inhibitors, An In Vitro Cytidine Deaminase Assay to Monitor APOBEC activity on DNA, Profiling rare C-to-U editing events via direct RNA sequencing, Global quantification of off-target activity by base editors, and so much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xiii 7. Preface - p. xvii 8. Chapter One: Fluorescent shift assay for APOBEC-mediated RNA editing - p. 1 9. Chapter Two: Low-error RNA sequencing techniques for detecting RNA editing by APOBECs: Circular RNAseq assay and safe-sequencing system (SSS) - p. 15 10. Chapter Three: Purification of enzymatically active APOBEC proteins from an insect cell expression system - p. 31 11. Chapter Four: In vitro deamination assay to measure the activity and processivity of AID/APOBEC enzymes - p. 69 12. Chapter Five: Defining the genome-wide mutagenic impact of APOBEC3 enzymes - p. 101 13. Chapter Six: Defining APOBEC-induced mutation signatures and modifying activities in yeast - p. 115 14. Chapter Seven: Biochemical assays for AID/APOBECs and the identification of AID/APOBEC inhibitors - p. 163 15. Chapter Eight: An in vitro cytidine deaminase assay to monitor APOBEC activity on DNA - p. 201 16. Chapter Nine: Profiling rare C-to-U editing events via direct RNA sequencing - p. 221 17. Chapter Ten: Global quantification of off-target activity by base editors - p. 255 18. Chapter Eleven: Restoration of cytidine to uridine genetic code using an MS2-APOBEC1 artificial enzymatic approach - p. 271 19. Chapter Twelve: Identification of RBP binding sites using RNA deaminases - p. 287 20. Chapter Thirteen: Programmable C-to-U editing to track endogenous proteins - p. 299
Apobec Enzymes, Volume 713 in this series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Fluorescent shift assay for APOBECs RNA editing, Low Error Sequencing Methods to Detect APOBEC-mediated RNA editing: Circular RNAseq and Safe-Sequencing System, "Safe-Barcode" RNAseq assay for APOBECs RNA editing, DT40 cell system to characterize somatic hypermutation, CH12 cell system to assay AID activity on class switch recombination, Purification of Enzymatically Active APOBEC Proteins from an Insect Cell Expression System, and more.
Additional chapters cover Defining genome-wide mutagenic impact of APOBEC3 enzymes, APOBEC-induced mutational assay in yeast, Assays for APOBEC drug discovery, Biochemical assay for the identification of APOBECs inhibitors, An In Vitro Cytidine Deaminase Assay to Monitor APOBEC activity on DNA, Profiling rare C-to-U editing events via direct RNA sequencing, Global quantification of off-target activity by base editors, and so much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xiii 7. Preface - p. xvii 8. Chapter One: Fluorescent shift assay for APOBEC-mediated RNA editing - p. 1 9. Chapter Two: Low-error RNA sequencing techniques for detecting RNA editing by APOBECs: Circular RNAseq assay and safe-sequencing system (SSS) - p. 15 10. Chapter Three: Purification of enzymatically active APOBEC proteins from an insect cell expression system - p. 31 11. Chapter Four: In vitro deamination assay to measure the activity and processivity of AID/APOBEC enzymes - p. 69 12. Chapter Five: Defining the genome-wide mutagenic impact of APOBEC3 enzymes - p. 101 13. Chapter Six: Defining APOBEC-induced mutation signatures and modifying activities in yeast - p. 115 14. Chapter Seven: Biochemical assays for AID/APOBECs and the identification of AID/APOBEC inhibitors - p. 163 15. Chapter Eight: An in vitro cytidine deaminase assay to monitor APOBEC activity on DNA - p. 201 16. Chapter Nine: Profiling rare C-to-U editing events via direct RNA sequencing - p. 221 17. Chapter Ten: Global quantification of off-target activity by base editors - p. 255 18. Chapter Eleven: Restoration of cytidine to uridine genetic code using an MS2-APOBEC1 artificial enzymatic approach - p. 271 19. Chapter Twelve: Identification of RBP binding sites using RNA deaminases - p. 287 20. Chapter Thirteen: Programmable C-to-U editing to track endogenous proteins - p. 299
