Bacterial Efflux Systems, Volume 722 covers experimental and computational approaches to study the function, structure, and mechanism of bacterial efflux pump systems. The methods covered include preparation of samples for structural and functional analyses and in vivo activity analysis. Specific chapters in this release cover Efflux pump functional assays, NMR and functional analysis efflux pumps, Methods for saturation mutagenesis and functional characterization, Cooperation and functional interplay between pumps, Mapping interactions, HDX-MS, and much more.
Other sections cover Recombinant Expression and purification, Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach, Reconstitution of rnd and mfs efflux pumps for structural biology, CusCBA system, Expression and purification of efflux pumps of Gram positives, Expression and purification of efflux pumps of archaea, ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc, In situ structural analysis of efflux assemblies by cryoET, Molecular dynamics simulations, and so much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xxi 8. Preface - p. xxvii 9. Section 1: Preparation and functional characterisation - p. 1 10. Chapter One: A robust protocol for refolding TolC and other outer membrane components of tripartite efflux pumps from inclusion bodies - p. 3 11. Chapter Two: Functional analysis and NMR studies of multi-drug efflux pumps - p. 21 12. Chapter Three: Using hydrogen/deuterium exchange mass spectrometry to understand bacterial membrane efflux proteins - p. 39 13. Chapter Four: Doxorubicin transport measurements for a bacterial multidrug transport protein - p. 83 14. Chapter Five: Molecular investigation of drug efflux pumps by deep mutational scanning - p. 99 15. Chapter Six: Reconstitution of tripartite efflux pumps MexAB-OprM and MacAB-TolC in biomimetic systems - p. 133 16. Chapter Seven: Preparation and activity characterization of a type IV ABC transporter efflux pump in peptidiscs - p. 159 17. Chapter Eight: Reconstitution and functional characterization of MmpL3, an essential transporter from Mycobacterium tuberculosis - p. 181 18. Chapter Nine: Discovery and functional characterization of archaeal efflux transporters - p. 211 19. Section 2: Structural and computational studies - p. 275 20. Chapter Ten: Crystallographic studies of efflux transporters with transport substrates and inhibitors - p. 277 21. Chapter Eleven: Computational approaches for modelling multidrug efflux pumps of the resistance nodulation-cell division superfamily - p. 299 22. Chapter Twelve: MexB/inhibitor complexes preparation for crystallography and cryo-EM, comparison and possible pitfalls - p. 363 23. Chapter Thirteen: Stabilization of multidrug efflux transporter MdfA and antibody fragments complex by cross-linking for Cryo-EM single-particle analysis - p. 395 24. Chapter Fourteen: Reconstitution of RND tripartite multidrug complexes for single-particle electron microscopy - p. 411 25. Chapter Fifteen: Cryo-electron microscopy (Cryo-EM) structural determination of the MmpL family of transporters - p. 437 26. Chapter Sixteen: In situ structural analysis of tripartite efflux assemblies by cryoET - p. 467 27. Section 3: Functional interplay and single cell heterogeneity - p. 485 28. Chapter Seventeen: Methods for investigating functional interplay between efflux pumps in Escherichia coli - p. 487 29. Chapter Eighteen: Quantitative single-cell imaging of efflux pump heterogeneity and antibiotic response dynamics - p. 501
Bacterial Efflux Systems, Volume 722 covers experimental and computational approaches to study the function, structure, and mechanism of bacterial efflux pump systems. The methods covered include preparation of samples for structural and functional analyses and in vivo activity analysis. Specific chapters in this release cover Efflux pump functional assays, NMR and functional analysis efflux pumps, Methods for saturation mutagenesis and functional characterization, Cooperation and functional interplay between pumps, Mapping interactions, HDX-MS, and much more.
Other sections cover Recombinant Expression and purification, Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach, Reconstitution of rnd and mfs efflux pumps for structural biology, CusCBA system, Expression and purification of efflux pumps of Gram positives, Expression and purification of efflux pumps of archaea, ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc, In situ structural analysis of efflux assemblies by cryoET, Molecular dynamics simulations, and so much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xxi 8. Preface - p. xxvii 9. Section 1: Preparation and functional characterisation - p. 1 10. Chapter One: A robust protocol for refolding TolC and other outer membrane components of tripartite efflux pumps from inclusion bodies - p. 3 11. Chapter Two: Functional analysis and NMR studies of multi-drug efflux pumps - p. 21 12. Chapter Three: Using hydrogen/deuterium exchange mass spectrometry to understand bacterial membrane efflux proteins - p. 39 13. Chapter Four: Doxorubicin transport measurements for a bacterial multidrug transport protein - p. 83 14. Chapter Five: Molecular investigation of drug efflux pumps by deep mutational scanning - p. 99 15. Chapter Six: Reconstitution of tripartite efflux pumps MexAB-OprM and MacAB-TolC in biomimetic systems - p. 133 16. Chapter Seven: Preparation and activity characterization of a type IV ABC transporter efflux pump in peptidiscs - p. 159 17. Chapter Eight: Reconstitution and functional characterization of MmpL3, an essential transporter from Mycobacterium tuberculosis - p. 181 18. Chapter Nine: Discovery and functional characterization of archaeal efflux transporters - p. 211 19. Section 2: Structural and computational studies - p. 275 20. Chapter Ten: Crystallographic studies of efflux transporters with transport substrates and inhibitors - p. 277 21. Chapter Eleven: Computational approaches for modelling multidrug efflux pumps of the resistance nodulation-cell division superfamily - p. 299 22. Chapter Twelve: MexB/inhibitor complexes preparation for crystallography and cryo-EM, comparison and possible pitfalls - p. 363 23. Chapter Thirteen: Stabilization of multidrug efflux transporter MdfA and antibody fragments complex by cross-linking for Cryo-EM single-particle analysis - p. 395 24. Chapter Fourteen: Reconstitution of RND tripartite multidrug complexes for single-particle electron microscopy - p. 411 25. Chapter Fifteen: Cryo-electron microscopy (Cryo-EM) structural determination of the MmpL family of transporters - p. 437 26. Chapter Sixteen: In situ structural analysis of tripartite efflux assemblies by cryoET - p. 467 27. Section 3: Functional interplay and single cell heterogeneity - p. 485 28. Chapter Seventeen: Methods for investigating functional interplay between efflux pumps in Escherichia coli - p. 487 29. Chapter Eighteen: Quantitative single-cell imaging of efflux pump heterogeneity and antibiotic response dynamics - p. 501
