For 40 years, Bancroft’s Theory and Practice of Histological Techniques has established itself as the standard reference for histotechnologists and laboratory scientists, as well as histopathologists. With coverage of the full range of histological techniques used in medical laboratories and pathology departments, it provides a strong foundation in all aspects of histological technology – from basic methods of section preparation and staining, to advanced diagnostic techniques such as immunocytochemistry and molecular testing. This revised and updated 8th Edition by Kim S. Suvarna, Christopher Layton, and John D. Bancroft is a one-stop reference for all those involved with histological preparations and applications, from student to highly advanced laboratory professional.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. Preface to the eighth edition - p. v 6. Preface to the first edition - p. vi 7. List of contributors - p. vii 8. Acknowledgments - p. ix 9. 1. Pathology laboratory management - p. 1 10. 2. Chemical safety in the laboratory - p. 12 11. 3. Light microscopy - p. 25 12. 4. Fixation of tissues - p. 40 13. 5. The gross room/surgical cut-up including sample handling - p. 64 14. 6. Tissue processing - p. 73 15. 7. Microtomy for paraffin and frozen sections - p. 84 16. 8. Resin (plastic) embedding for microscopy and tissue analysis - p. 96 17. 9. Theory of histological staining - p. 114 18. 10. The hematoxylins and eosin - p. 126 19. 11. Automation in the histology department - p. 139 20. 12. Connective and other mesenchymal tissues with their stains - p. 153 21. 13. Carbohydrates - p. 176 22. 14. Pigments and minerals - p. 198 23. 15. Amyloid - p. 231 24. 16. Traditional stains and modern techniques for demonstrating microorganisms in histology - p. 254 25. 17. Bone - p. 280 26. 18. Neuropathology and muscle biopsy techniques - p. 306 27. 19. Immunohistochemical and immunofluorescent techniques - p. 337 28. 20. Molecular pathology - p. 395 29. 21. Transmission electron microscopy - p. 434 30. 22. Digital pathology - p. 476 31. Appendices - p. 492 32. Appendix I. Traditional methods - p. 495 33. Appendix II. Tissue microarray - p. 505 34. Appendix III. Applications of immunohistochemistry - p. 509 35. Appendix IV. Measurement units - p. 519 36. Appendix V. Preparation of solutions - p. 521 37. Appendix VI. Buffer solutions - p. 525 38. Appendix VII. Solubility of some common reagents and dyes - p. 529 39. Appendix VIII. Mounting media and slide coatings - p. 535 40. Index - p. 537
For 40 years, Bancroft’s Theory and Practice of Histological Techniques has established itself as the standard reference for histotechnologists and laboratory scientists, as well as histopathologists. With coverage of the full range of histological techniques used in medical laboratories and pathology departments, it provides a strong foundation in all aspects of histological technology – from basic methods of section preparation and staining, to advanced diagnostic techniques such as immunocytochemistry and molecular testing. This revised and updated 8th Edition by Kim S. Suvarna, Christopher Layton, and John D. Bancroft is a one-stop reference for all those involved with histological preparations and applications, from student to highly advanced laboratory professional.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. Preface to the eighth edition - p. v 6. Preface to the first edition - p. vi 7. List of contributors - p. vii 8. Acknowledgments - p. ix 9. 1. Pathology laboratory management - p. 1 10. 2. Chemical safety in the laboratory - p. 12 11. 3. Light microscopy - p. 25 12. 4. Fixation of tissues - p. 40 13. 5. The gross room/surgical cut-up including sample handling - p. 64 14. 6. Tissue processing - p. 73 15. 7. Microtomy for paraffin and frozen sections - p. 84 16. 8. Resin (plastic) embedding for microscopy and tissue analysis - p. 96 17. 9. Theory of histological staining - p. 114 18. 10. The hematoxylins and eosin - p. 126 19. 11. Automation in the histology department - p. 139 20. 12. Connective and other mesenchymal tissues with their stains - p. 153 21. 13. Carbohydrates - p. 176 22. 14. Pigments and minerals - p. 198 23. 15. Amyloid - p. 231 24. 16. Traditional stains and modern techniques for demonstrating microorganisms in histology - p. 254 25. 17. Bone - p. 280 26. 18. Neuropathology and muscle biopsy techniques - p. 306 27. 19. Immunohistochemical and immunofluorescent techniques - p. 337 28. 20. Molecular pathology - p. 395 29. 21. Transmission electron microscopy - p. 434 30. 22. Digital pathology - p. 476 31. Appendices - p. 492 32. Appendix I. Traditional methods - p. 495 33. Appendix II. Tissue microarray - p. 505 34. Appendix III. Applications of immunohistochemistry - p. 509 35. Appendix IV. Measurement units - p. 519 36. Appendix V. Preparation of solutions - p. 521 37. Appendix VI. Buffer solutions - p. 525 38. Appendix VII. Solubility of some common reagents and dyes - p. 529 39. Appendix VIII. Mounting media and slide coatings - p. 535 40. Index - p. 537
