Basic Biotechnique for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from basic laboratory techniques, to scale-up, process development, demonstration and commercialization. The book compiles currently scattered materials on the topic and updates information based on practical experience and requirements.
1. Title of Book - p. Cover 2. Cover image - p. Cover 3. Title page - p. ii 4. Table of Contents - p. iv 5. Copyright - p. iv 6. Contributors - p. xv 7. Foreword - p. xviii 8. Preface - p. xviii 9. Part I: Isolation, screening and culture maintenance - p. xviii 10. Chapter 1. Isolation of microorganisms - p. 5 11. Chapter 2. Screening strategies - p. 23 12. Chapter 3. Identification, morphological, biochemical, and genetic characterization of microorganisms - p. 47 13. Chapter 4. Microbial activity and productivity enhancement strategies - p. 85 14. Chapter 5. Culture maintenance, preservation, and strain improvement - p. 105 15. Part II: Laboratory techniques & instrumentation - p. 121 16. Chapter 6. Biomolecules: Types, homogenization, bead beater, sonication - p. 126 17. Chapter 7. Centrifugation: Basic principle, types - p. 133 18. Chapter 8. Spectroscopy—Principle, types, and applications - p. 145 19. Chapter 9. Protein purification: Basic principles and techniques - p. 165 20. Chapter 10. Chromatography: Basic principle, types, and applications - p. 173 21. Chapter 11. Electrophoresis: Basic principle, types, and applications - p. 183 22. Part III: Genomic and proteomic analysis - p. 193 23. Chapter 12. DNA, RNA isolation, primer designing, sequence submission, and phylogenetic analysis - p. 197 24. Chapter 13. RDT and genetic engineering: Basic of RDT method, PCR, and application - p. 212 25. Chapter 14. Protein sequence analysis - p. 217 26. Chapter 15. Computational strategies and tools for protein tertiary structure prediction - p. 228 27. Chapter 16. Docking strategies - p. 244 28. Chapter 17. A beginner’s guide to measuring binding affinity during biomolecular interactions - p. 259 29. Part IV: Techniques in process development - p. 273 30. Chapter 18. Enzymes and their significance in the industrial bioprocesses - p. 274 31. Chapter 19. Enzyme kinetics: Industrially important enzymes - p. 290 32. Chapter 20. Industrial enzymes: Basic information, assay, and applications - p. 295 33. Part V: Techniques in process development - p. 309 34. Chapter 21. Fundamentals of fermentation technology - p. 313 35. Chapter 22. Sterilization in bioprocesses - p. 329 36. Chapter 23. Scale-up: Lab to commercial scale - p. 341 37. Chapter 24. Bioprocess: Control, management, and biosafety issues - p. 355 38. Chapter 25. Demonstration and industrial scale-up - p. 365 39. Chapter 26. Downstream processing of biotechnology products - p. 377 40. Chapter 27. Waste management and environment - p. 391 41. Part VI: Feasibility, marketing and biobusiness - p. 413 42. Chapter 28. Biobusiness opportunities - p. 417 43. Chapter 29. Feasibility analysis and business plan - p. 427 44. Chapter 30. Patenting, IPR, and regulatory issues - p. 441 45. Chapter 31. Commercialization and technology transfers of bioprocess - p. 455 46. Chapter 32. Bioproduct marketing strategies - p. 471 47. Index - p. 485
Basic Biotechnique for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from basic laboratory techniques, to scale-up, process development, demonstration and commercialization. The book compiles currently scattered materials on the topic and updates information based on practical experience and requirements.
1. Title of Book - p. Cover 2. Cover image - p. Cover 3. Title page - p. ii 4. Table of Contents - p. iv 5. Copyright - p. iv 6. Contributors - p. xv 7. Foreword - p. xviii 8. Preface - p. xviii 9. Part I: Isolation, screening and culture maintenance - p. xviii 10. Chapter 1. Isolation of microorganisms - p. 5 11. Chapter 2. Screening strategies - p. 23 12. Chapter 3. Identification, morphological, biochemical, and genetic characterization of microorganisms - p. 47 13. Chapter 4. Microbial activity and productivity enhancement strategies - p. 85 14. Chapter 5. Culture maintenance, preservation, and strain improvement - p. 105 15. Part II: Laboratory techniques & instrumentation - p. 121 16. Chapter 6. Biomolecules: Types, homogenization, bead beater, sonication - p. 126 17. Chapter 7. Centrifugation: Basic principle, types - p. 133 18. Chapter 8. Spectroscopy—Principle, types, and applications - p. 145 19. Chapter 9. Protein purification: Basic principles and techniques - p. 165 20. Chapter 10. Chromatography: Basic principle, types, and applications - p. 173 21. Chapter 11. Electrophoresis: Basic principle, types, and applications - p. 183 22. Part III: Genomic and proteomic analysis - p. 193 23. Chapter 12. DNA, RNA isolation, primer designing, sequence submission, and phylogenetic analysis - p. 197 24. Chapter 13. RDT and genetic engineering: Basic of RDT method, PCR, and application - p. 212 25. Chapter 14. Protein sequence analysis - p. 217 26. Chapter 15. Computational strategies and tools for protein tertiary structure prediction - p. 228 27. Chapter 16. Docking strategies - p. 244 28. Chapter 17. A beginner’s guide to measuring binding affinity during biomolecular interactions - p. 259 29. Part IV: Techniques in process development - p. 273 30. Chapter 18. Enzymes and their significance in the industrial bioprocesses - p. 274 31. Chapter 19. Enzyme kinetics: Industrially important enzymes - p. 290 32. Chapter 20. Industrial enzymes: Basic information, assay, and applications - p. 295 33. Part V: Techniques in process development - p. 309 34. Chapter 21. Fundamentals of fermentation technology - p. 313 35. Chapter 22. Sterilization in bioprocesses - p. 329 36. Chapter 23. Scale-up: Lab to commercial scale - p. 341 37. Chapter 24. Bioprocess: Control, management, and biosafety issues - p. 355 38. Chapter 25. Demonstration and industrial scale-up - p. 365 39. Chapter 26. Downstream processing of biotechnology products - p. 377 40. Chapter 27. Waste management and environment - p. 391 41. Part VI: Feasibility, marketing and biobusiness - p. 413 42. Chapter 28. Biobusiness opportunities - p. 417 43. Chapter 29. Feasibility analysis and business plan - p. 427 44. Chapter 30. Patenting, IPR, and regulatory issues - p. 441 45. Chapter 31. Commercialization and technology transfers of bioprocess - p. 455 46. Chapter 32. Bioproduct marketing strategies - p. 471 47. Index - p. 485
