Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes.
Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.
1. Biocatalyst Immobilization - p. i 2. Cover - p. i 3. Title Page - p. ii 4. Copyright - p. iv 5. Table of Contents - p. iv 6. Dedication - p. v 7. Contributors - p. xix 8. Preface - p. xxiii 9. Acknowledgments - p. xxv 10. Chapter 1 The enzyme, the support, and the immobilization strategy: The key findings to a desirable biocatalyst - p. 1 11. Chapter 2 Selection and modification of enzymes prior to immobilization - p. 17 12. Chapter 3 Enzyme immobilization approaches - p. 37 13. Chapter 4 Postimmobilization treatments before applications - p. 55 14. Chapter 5 Support-free immobilization - p. 87 15. Chapter 6 Measuring and reporting enzyme's immobilization efficiency - p. 115 16. Chapter 7 Some recent innovations related to enzyme immobilization - p. 149 17. Chapter 8 Enzyme immobilization for use in nonconventional media - p. 165 18. Chapter 9 Assaying the biological activities of immobilized enzymes - p. 203 19. Chapter 10 Enzyme immobilization on nanomaterials and nanostructured supports - p. 231 20. Chapter 11 Oriented immobilization of biomolecules on small surfaces - p. 249 21. Chapter 12 Affinity immobilization and affinity layers - p. 269 22. Chapter 13 New enzymatic reactor designs: From enzymatic batch to 3D microreactors and monoliths - p. 291 23. Chapter 14 Immobilization of multienzymes: Problems and solutions - p. 316 24. Chapter 15 Enzymatic biosensors - p. 341 25. Chapter 16 Industrial applications of immobilized enzymes: Food and other areas - p. 365 26. Chapter 17 Future perspectives in enzyme immobilization - p. 403 27. Index - p. 427
Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes.
Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.
1. Biocatalyst Immobilization - p. i 2. Cover - p. i 3. Title Page - p. ii 4. Copyright - p. iv 5. Table of Contents - p. iv 6. Dedication - p. v 7. Contributors - p. xix 8. Preface - p. xxiii 9. Acknowledgments - p. xxv 10. Chapter 1 The enzyme, the support, and the immobilization strategy: The key findings to a desirable biocatalyst - p. 1 11. Chapter 2 Selection and modification of enzymes prior to immobilization - p. 17 12. Chapter 3 Enzyme immobilization approaches - p. 37 13. Chapter 4 Postimmobilization treatments before applications - p. 55 14. Chapter 5 Support-free immobilization - p. 87 15. Chapter 6 Measuring and reporting enzyme's immobilization efficiency - p. 115 16. Chapter 7 Some recent innovations related to enzyme immobilization - p. 149 17. Chapter 8 Enzyme immobilization for use in nonconventional media - p. 165 18. Chapter 9 Assaying the biological activities of immobilized enzymes - p. 203 19. Chapter 10 Enzyme immobilization on nanomaterials and nanostructured supports - p. 231 20. Chapter 11 Oriented immobilization of biomolecules on small surfaces - p. 249 21. Chapter 12 Affinity immobilization and affinity layers - p. 269 22. Chapter 13 New enzymatic reactor designs: From enzymatic batch to 3D microreactors and monoliths - p. 291 23. Chapter 14 Immobilization of multienzymes: Problems and solutions - p. 316 24. Chapter 15 Enzymatic biosensors - p. 341 25. Chapter 16 Industrial applications of immobilized enzymes: Food and other areas - p. 365 26. Chapter 17 Future perspectives in enzyme immobilization - p. 403 27. Index - p. 427
