Biomolecular Kinetics bridges the gap between fundamental biochemistry and practical bioprocess applications. The book offers a clear and logical exploration of biomolecules, their relationships to cellular functions, and the formulation of fermentation media. With detailed derivations and visual aids, readers will swiftly grasp the essentials of cell chemistry, reaction rates, and the complexities of bioprocess engineering. The content is well-organized, beginning with an introduction to biological basics and progressing through key topics such as carbohydrates, nucleic acids, proteins, lipids, and metabolites. Readers will gain insights into cell feed formulation, chemical reaction principles, enzyme kinetics, and the design of experiments. Each chapter builds on the last, providing a thorough understanding of the mechanistic pathways of bioreactions and the quantitative analysis of bioprocess parameters.
Biomolecular Kinetics is an invaluable resource for students, researchers, and professionals in biochemistry, biotechnology, and bioprocess engineering.
1. Chapter 1. An introduction to biological basics - p. 1 2. Chapter 2. Carbohydrate - p. 25 3. Chapter 3. Nucleic acids - p. 67 4. Chapter 4. Amino acid and protein - p. 87 5. Chapter 5. Lipid - p. 121 6. Chapter 6. Metabolites - p. 165 7. Chapter 7. Cell feed - p. 211 8. Chapter 8. Principles of chemical reactions - p. 239 9. Chapter 9. Chemical kinetics theory - p. 269 10. Chapter 10. Reaction rate data analysis - p. 295 11. Chapter 11. Enzymes - p. 323 12. Chapter 12. Protein bioactivation - p. 369 13. Abstract - p. 369 14. 12.1 Multifunctionalization of proteins - p. 371 15. 12.2 Covalently bound oligomers - p. 371 16. 12.3 Noncovalent assembly of protein - p. 376 17. 12.4 Protein assembly through domain swapping - p. 382 18. 12.5 Coexistence of protein oligomer mixtures - p. 386 19. 12.6 Protein–ligand interactions - p. 390 20. 12.7 Single ligand species on homosteric enzymes - p. 393 21. 12.8 Single ligand species in allosteric enzymes - p. 400 22. 12.9 Multiple different ligand-specific active centers - p. 405 23. 12.10 Competitive ligand interactions on a homosteric enzyme - p. 409 24. 12.11 Simplistic stiff homosteric enzyme bimorph models - p. 411 25. 12.12 Summary - p. 414 26. 12.13 Problems - p. 415 27. Further reading - p. 416 28. Chapter 13. Complex enzyme kinetics - p. 417 29. Abstract - p. 417 30. 13.1 Allosteric interaction - p. 418 31. 13.2 Single substrate reactions: the initial rates - p. 424 32. 13.3 “Unimolecular” reactions - p. 436 33. 13.4 Bimolecular reactions - p. 439 34. 13.5 The simplest polymorph: a bimorph of two monomeric forms of the same enzyme - p. 446 35. 13.6 Kinetics of polymorphic enzyme catalysis - p. 452 36. 13.7 Summary - p. 459 37. 13.8 Problems - p. 460 38. Further reading - p. 467 39. Chapter 14. Cell metabolism - p. 469 40. Chapter 15. Cell genetics and stability - p. 513 41. Chapter 16. Cell kinetics - p. 537 42. Abstract - p. 537 43. 16.1 Quantifying biomass - p. 538 44. 16.2 Batch growth patterns - p. 540 45. 16.3 Biomass yield - p. 544 46. 16.4 Growth kinetics—“Empirical Approximation” of metabolic pathway - p. 546 47. 16.5 Cell death rate, cell maintenance, and endogenous metabolism - p. 551 48. 16.6 Product yield/rate and overall substrate rate - p. 558 49. 16.7 Process analytical technology - p. 559 50. 16.8 Overview of kinetic models for cell cultivation - p. 569 51. 16.9 Selective substrate uptake kinetics - p. 584 52. 16.10 Summary - p. 590 53. 16.11 Problems - p. 595 54. References - p. 599 55. Further reading - p. 599 56. Chapter 17. Catalysis on solid surfaces - p. 601 57. Abstract - p. 601 58. 17.1 Catalysis - p. 601 59. 17.2 How does reaction with solids occur? - p. 604 60. 17.3 Langmuir: theoretical basis of adsorption kinetics - p. 607 61. 17.4 Idealization of nonideal surfaces - p. 611 62. 17.5 Cooperative adsorption - p. 620 63. 17.6 LHHW: surface reactions with rate-controlling steps - p. 633 64. 17.7 Why do rate approximations such as LHHW works? - p. 638 65. 17.8 Chemical reactions on nonideal surfaces based on the distribution of interaction energy - p. 647 66. 17.9 Cooperative catalysis - p. 650 67. 17.10 Kinetics of reactions on surfaces where the solid is either a product or a reactant - p. 652 68. 17.11 Decline of surface activity: catalyst deactivation - p. 654 69. 17.12 Summary - p. 654 70. 17.13 Problems - p. 658 71. Further reading - p. 662 72. Chapter 18. Design of experiment - p. 663 73. Index - p. 717
Biomolecular Kinetics bridges the gap between fundamental biochemistry and practical bioprocess applications. The book offers a clear and logical exploration of biomolecules, their relationships to cellular functions, and the formulation of fermentation media. With detailed derivations and visual aids, readers will swiftly grasp the essentials of cell chemistry, reaction rates, and the complexities of bioprocess engineering. The content is well-organized, beginning with an introduction to biological basics and progressing through key topics such as carbohydrates, nucleic acids, proteins, lipids, and metabolites. Readers will gain insights into cell feed formulation, chemical reaction principles, enzyme kinetics, and the design of experiments. Each chapter builds on the last, providing a thorough understanding of the mechanistic pathways of bioreactions and the quantitative analysis of bioprocess parameters.
Biomolecular Kinetics is an invaluable resource for students, researchers, and professionals in biochemistry, biotechnology, and bioprocess engineering.
1. Chapter 1. An introduction to biological basics - p. 1 2. Chapter 2. Carbohydrate - p. 25 3. Chapter 3. Nucleic acids - p. 67 4. Chapter 4. Amino acid and protein - p. 87 5. Chapter 5. Lipid - p. 121 6. Chapter 6. Metabolites - p. 165 7. Chapter 7. Cell feed - p. 211 8. Chapter 8. Principles of chemical reactions - p. 239 9. Chapter 9. Chemical kinetics theory - p. 269 10. Chapter 10. Reaction rate data analysis - p. 295 11. Chapter 11. Enzymes - p. 323 12. Chapter 12. Protein bioactivation - p. 369 13. Abstract - p. 369 14. 12.1 Multifunctionalization of proteins - p. 371 15. 12.2 Covalently bound oligomers - p. 371 16. 12.3 Noncovalent assembly of protein - p. 376 17. 12.4 Protein assembly through domain swapping - p. 382 18. 12.5 Coexistence of protein oligomer mixtures - p. 386 19. 12.6 Protein–ligand interactions - p. 390 20. 12.7 Single ligand species on homosteric enzymes - p. 393 21. 12.8 Single ligand species in allosteric enzymes - p. 400 22. 12.9 Multiple different ligand-specific active centers - p. 405 23. 12.10 Competitive ligand interactions on a homosteric enzyme - p. 409 24. 12.11 Simplistic stiff homosteric enzyme bimorph models - p. 411 25. 12.12 Summary - p. 414 26. 12.13 Problems - p. 415 27. Further reading - p. 416 28. Chapter 13. Complex enzyme kinetics - p. 417 29. Abstract - p. 417 30. 13.1 Allosteric interaction - p. 418 31. 13.2 Single substrate reactions: the initial rates - p. 424 32. 13.3 “Unimolecular” reactions - p. 436 33. 13.4 Bimolecular reactions - p. 439 34. 13.5 The simplest polymorph: a bimorph of two monomeric forms of the same enzyme - p. 446 35. 13.6 Kinetics of polymorphic enzyme catalysis - p. 452 36. 13.7 Summary - p. 459 37. 13.8 Problems - p. 460 38. Further reading - p. 467 39. Chapter 14. Cell metabolism - p. 469 40. Chapter 15. Cell genetics and stability - p. 513 41. Chapter 16. Cell kinetics - p. 537 42. Abstract - p. 537 43. 16.1 Quantifying biomass - p. 538 44. 16.2 Batch growth patterns - p. 540 45. 16.3 Biomass yield - p. 544 46. 16.4 Growth kinetics—“Empirical Approximation” of metabolic pathway - p. 546 47. 16.5 Cell death rate, cell maintenance, and endogenous metabolism - p. 551 48. 16.6 Product yield/rate and overall substrate rate - p. 558 49. 16.7 Process analytical technology - p. 559 50. 16.8 Overview of kinetic models for cell cultivation - p. 569 51. 16.9 Selective substrate uptake kinetics - p. 584 52. 16.10 Summary - p. 590 53. 16.11 Problems - p. 595 54. References - p. 599 55. Further reading - p. 599 56. Chapter 17. Catalysis on solid surfaces - p. 601 57. Abstract - p. 601 58. 17.1 Catalysis - p. 601 59. 17.2 How does reaction with solids occur? - p. 604 60. 17.3 Langmuir: theoretical basis of adsorption kinetics - p. 607 61. 17.4 Idealization of nonideal surfaces - p. 611 62. 17.5 Cooperative adsorption - p. 620 63. 17.6 LHHW: surface reactions with rate-controlling steps - p. 633 64. 17.7 Why do rate approximations such as LHHW works? - p. 638 65. 17.8 Chemical reactions on nonideal surfaces based on the distribution of interaction energy - p. 647 66. 17.9 Cooperative catalysis - p. 650 67. 17.10 Kinetics of reactions on surfaces where the solid is either a product or a reactant - p. 652 68. 17.11 Decline of surface activity: catalyst deactivation - p. 654 69. 17.12 Summary - p. 654 70. 17.13 Problems - p. 658 71. Further reading - p. 662 72. Chapter 18. Design of experiment - p. 663 73. Index - p. 717
