Biorthogonal Tagging, Volume 731 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters such as Staudinger Ligations and Related Reactions: Scope and Applications in Chemical Biology, Strain-Promoted Bioorthogonal Cycloadditions to Investigate Sialyltransferases Promiscuity Towards Unnatural Sialic Acids, Practical Guidelines for the Bioorthogonal AMI–Isonitrile Ligation, Nitrones and Other Dipoles in Bioorthogonal Chemistry Applications, 1,2-Aminothiol-Specific Conjugation for Dual-Color Fluorescent Labeling via Ultrafast TAMMs, Genetic Incorporation of Bicyclononyne for Cell-Surface Protein Labeling via Tetrazine Ligation, and much more.
Additional chapters cover Genetic Code Expansion for Single Noncanonical Amino Acid Incorporation into SARS-CoV-2 Omicron Spike on Amber-Free Lentiviral Particles, Bioorthogonal Click-Chemistry Labeling and Visualization of Dual Noncanonical Amino Acid–Incorporated HIV-1 Env on Intact Virions, Studying Bacterial Glycans with Azidosugars and Bioorthogonal Chemistry, Bioorthogonal Tagging of DNA and RNA, Efficient Labeling of Pathogenic Fungal Chitin with Bioorthogonal Probes, and many more timely topics.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xxiii 8. Chapter One: Biorthogonal tagging Staudinger ligations and related reactions – scope and applications in chemical biology - p. 1 9. Chapter Two: Strain-promoted bioorthogonal cycloadditions to investigate sialyltransferases promiscuity towards unnatural sialic acids - p. 31 10. Chapter Three: Practical guidelines for the AMI-Isonitrile ligation - p. 45 11. Chapter Four: Nitrone dipoles in bioorthogonal chemistry applications - p. 73 12. Chapter Five: 1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates - p. 99 13. Chapter Six: Genetic incorporation of a BCN-bearing amino acid for cell-surface protein labeling via tetrazine ligation - p. 119 14. Chapter Seven: Genetic code expansion for single noncanonical amino acid incorporation into SARS-CoV-2 Omicron spike on amber-free lentiviral particles - p. 141 15. Chapter Eight: Bioorthogonal click-chemistry labeling and visualization of dual noncanonical amino acid-incorporated HIV-1 Env on intact virions - p. 167 16. Chapter Nine: Studying bacterial glycans with azidosugars and bioorthogonal chemistry - p. 193 17. Chapter Ten: Chemoenzymatic labeling of RNA using terminal uridylyl transferase and bioorthogonal click chemistry - p. 223 18. Chapter Eleven: Efficient labeling of pathogenic fungal chitin with bioorthogonal probes - p. 253 19. Chapter Twelve: Metabolic labeling and targeting of dendritic cells - p. 285 20. Chapter Thirteen: Orthogonal crosslinking of monobodies using a genetically encoded β-lactam amino acid - p. 301 21. Chapter Fourteen: Bioorthogonal chemical labeling of endogenous receptors in the live mouse brain - p. 317 22. Chapter Fifteen: Organelle-directed metabolic glycan labeling - p. 335 23. Chapter Sixteen: Molecular imaging enabled by bioorthogonal click labeling - p. 353 24. Chapter Seventeen: Molecular engineering of activatable cyclopropenes for bioorthogonal labeling - p. 387 25. Chapter Eighteen: Pyrone and pyranthione scaffolds for click-to-release and biological gas delivery - p. 427 26. Chapter Nineteen: Click and release through bioorthogonal hydroamination - p. 463 27. Chapter Twenty: Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling - p. 485 28. Chapter Twenty One: inCu-click: Enabling copper-catalyzed click chemistry inside living cells - p. 503 29. Chapter Twenty Two: Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell–cell interactions - p. 529 30. Chapter Twenty Three: Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy - p. 555
Biorthogonal Tagging, Volume 731 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters such as Staudinger Ligations and Related Reactions: Scope and Applications in Chemical Biology, Strain-Promoted Bioorthogonal Cycloadditions to Investigate Sialyltransferases Promiscuity Towards Unnatural Sialic Acids, Practical Guidelines for the Bioorthogonal AMI–Isonitrile Ligation, Nitrones and Other Dipoles in Bioorthogonal Chemistry Applications, 1,2-Aminothiol-Specific Conjugation for Dual-Color Fluorescent Labeling via Ultrafast TAMMs, Genetic Incorporation of Bicyclononyne for Cell-Surface Protein Labeling via Tetrazine Ligation, and much more.
Additional chapters cover Genetic Code Expansion for Single Noncanonical Amino Acid Incorporation into SARS-CoV-2 Omicron Spike on Amber-Free Lentiviral Particles, Bioorthogonal Click-Chemistry Labeling and Visualization of Dual Noncanonical Amino Acid–Incorporated HIV-1 Env on Intact Virions, Studying Bacterial Glycans with Azidosugars and Bioorthogonal Chemistry, Bioorthogonal Tagging of DNA and RNA, Efficient Labeling of Pathogenic Fungal Chitin with Bioorthogonal Probes, and many more timely topics.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xxiii 8. Chapter One: Biorthogonal tagging Staudinger ligations and related reactions – scope and applications in chemical biology - p. 1 9. Chapter Two: Strain-promoted bioorthogonal cycloadditions to investigate sialyltransferases promiscuity towards unnatural sialic acids - p. 31 10. Chapter Three: Practical guidelines for the AMI-Isonitrile ligation - p. 45 11. Chapter Four: Nitrone dipoles in bioorthogonal chemistry applications - p. 73 12. Chapter Five: 1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates - p. 99 13. Chapter Six: Genetic incorporation of a BCN-bearing amino acid for cell-surface protein labeling via tetrazine ligation - p. 119 14. Chapter Seven: Genetic code expansion for single noncanonical amino acid incorporation into SARS-CoV-2 Omicron spike on amber-free lentiviral particles - p. 141 15. Chapter Eight: Bioorthogonal click-chemistry labeling and visualization of dual noncanonical amino acid-incorporated HIV-1 Env on intact virions - p. 167 16. Chapter Nine: Studying bacterial glycans with azidosugars and bioorthogonal chemistry - p. 193 17. Chapter Ten: Chemoenzymatic labeling of RNA using terminal uridylyl transferase and bioorthogonal click chemistry - p. 223 18. Chapter Eleven: Efficient labeling of pathogenic fungal chitin with bioorthogonal probes - p. 253 19. Chapter Twelve: Metabolic labeling and targeting of dendritic cells - p. 285 20. Chapter Thirteen: Orthogonal crosslinking of monobodies using a genetically encoded β-lactam amino acid - p. 301 21. Chapter Fourteen: Bioorthogonal chemical labeling of endogenous receptors in the live mouse brain - p. 317 22. Chapter Fifteen: Organelle-directed metabolic glycan labeling - p. 335 23. Chapter Sixteen: Molecular imaging enabled by bioorthogonal click labeling - p. 353 24. Chapter Seventeen: Molecular engineering of activatable cyclopropenes for bioorthogonal labeling - p. 387 25. Chapter Eighteen: Pyrone and pyranthione scaffolds for click-to-release and biological gas delivery - p. 427 26. Chapter Nineteen: Click and release through bioorthogonal hydroamination - p. 463 27. Chapter Twenty: Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling - p. 485 28. Chapter Twenty One: inCu-click: Enabling copper-catalyzed click chemistry inside living cells - p. 503 29. Chapter Twenty Two: Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell–cell interactions - p. 529 30. Chapter Twenty Three: Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy - p. 555
