Biophysical Approaches for the Study of Membrane Structure, Part A, Volume 700 explores lipid membrane asymmetry and lateral heterogeneity. A burst of recent research has shown that bilayers whose leaflets differ in their physical properties—such as composition, phase state, or lateral stress—exhibit many fascinating new characteristics, but also pose a host of new challenges related to their creation, characterization, simulation, and theoretical description. Chapters in this new release include Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCS, Effects of lateral and hydrostatic pressure on membrane structure and properties, and much more.
Other sections cover Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cells, Direct quantification of cellular membrane lipids using ratiometric fluorescence sensors, The spectral phasor approach to resolving membrane order with environmentally sensitive dyes, The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains, Advanced microscopy methods to study membrane pores, Use of cryo-EM to study membrane phase separation, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging fluorescence correlation spectroscopy - p. 2 9. Chapter Two: Fluorescence imaging of lamellipodin-mediated biomolecular condensates on solid supported lipid bilayer membranes - p. 34 10. Chapter Three: The effect of hydrostatic pressure on lipid membrane lateral structure - p. 50 11. Chapter Four: Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells - p. 79 12. Chapter Five: The spectral phasor approach to resolving membrane order with environmentally sensitive dyes - p. 106 13. Chapter Six: The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains - p. 129 14. Chapter Seven: Super-resolution microscopy methods to study membrane pores in situ - p. 162 15. Chapter Eight: Phase separation in model lipid membranes investigated with cryogenic electron microscopy - p. 190 16. Chapter Nine: Using lipid binding proteins and advanced microscopy to study lipid domains - p. 218 17. Chapter Ten: Structural characterization of lateral phase separation in polymer–lipid hybrid membranes - p. 237 18. Chapter Eleven: Applications of phase-separating multi-bilayers in protein-membrane domain interactions - p. 276 19. Chapter Twelve: Studying lipid flip-flop in asymmetric liposomes using 1H NMR and TR-SANS - p. 296 20. Chapter Thirteen: Thickness determination of hydroperoxidized lipid bilayers from medium-resolution cryo-TEM images - p. 330 21. Chapter Fourteen: Structure of symmetric and asymmetric lipid membranes from joint SAXS/SANS - p. 350 22. Chapter Fifteen: Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopy - p. 387 23. Chapter Sixteen: A live cell imaging-based assay for tracking particle uptake by clathrin-mediated endocytosis - p. 415 24. Chapter Seventeen: Pore-spanning membranes as a tool to investigate lateral lipid membrane heterogeneity - p. 456 25. Chapter Eighteen: MαCD-based plasma membrane outer leaflet lipid exchange in mammalian cells to study insulin receptor activity - p. 487
Biophysical Approaches for the Study of Membrane Structure, Part A, Volume 700 explores lipid membrane asymmetry and lateral heterogeneity. A burst of recent research has shown that bilayers whose leaflets differ in their physical properties—such as composition, phase state, or lateral stress—exhibit many fascinating new characteristics, but also pose a host of new challenges related to their creation, characterization, simulation, and theoretical description. Chapters in this new release include Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCS, Effects of lateral and hydrostatic pressure on membrane structure and properties, and much more.
Other sections cover Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cells, Direct quantification of cellular membrane lipids using ratiometric fluorescence sensors, The spectral phasor approach to resolving membrane order with environmentally sensitive dyes, The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains, Advanced microscopy methods to study membrane pores, Use of cryo-EM to study membrane phase separation, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging fluorescence correlation spectroscopy - p. 2 9. Chapter Two: Fluorescence imaging of lamellipodin-mediated biomolecular condensates on solid supported lipid bilayer membranes - p. 34 10. Chapter Three: The effect of hydrostatic pressure on lipid membrane lateral structure - p. 50 11. Chapter Four: Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells - p. 79 12. Chapter Five: The spectral phasor approach to resolving membrane order with environmentally sensitive dyes - p. 106 13. Chapter Six: The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains - p. 129 14. Chapter Seven: Super-resolution microscopy methods to study membrane pores in situ - p. 162 15. Chapter Eight: Phase separation in model lipid membranes investigated with cryogenic electron microscopy - p. 190 16. Chapter Nine: Using lipid binding proteins and advanced microscopy to study lipid domains - p. 218 17. Chapter Ten: Structural characterization of lateral phase separation in polymer–lipid hybrid membranes - p. 237 18. Chapter Eleven: Applications of phase-separating multi-bilayers in protein-membrane domain interactions - p. 276 19. Chapter Twelve: Studying lipid flip-flop in asymmetric liposomes using 1H NMR and TR-SANS - p. 296 20. Chapter Thirteen: Thickness determination of hydroperoxidized lipid bilayers from medium-resolution cryo-TEM images - p. 330 21. Chapter Fourteen: Structure of symmetric and asymmetric lipid membranes from joint SAXS/SANS - p. 350 22. Chapter Fifteen: Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopy - p. 387 23. Chapter Sixteen: A live cell imaging-based assay for tracking particle uptake by clathrin-mediated endocytosis - p. 415 24. Chapter Seventeen: Pore-spanning membranes as a tool to investigate lateral lipid membrane heterogeneity - p. 456 25. Chapter Eighteen: MαCD-based plasma membrane outer leaflet lipid exchange in mammalian cells to study insulin receptor activity - p. 487
