Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges.
This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques.
1. Title of Book - p. Cover 2. Cover image - p. Cover 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Contributors - p. xi 7. Preface - p. xv 8. Chapter 1. Derivatization of carbohydrates for analysis by liquid chromatography and capillary electrophoresis - p. 6 9. Chapter 2. Reversed-phase and hydrophobic interaction chromatography of carbohydrates and glycoconjugates - p. 35 10. Chapter 3. HPLC of carbohydrates with cation-exchange silica and resin-based stationary phases - p. 146 11. Chapter 4. High-performance anion-exchange chromatography with pulsed amperometric detection for carbohydrate and glycoconjugate analyses - p. 157 12. Chapter 5. Hydrophilic interaction liquid chromatography-mass spectrometry for the characterization of glycoproteins at the glycan, peptide, subunit, and intact level - p. 218 13. Chapter 6. Frontal affinity chromatography: A unique approach for weak interaction analysis targeting lectins and oligosaccharides - p. 279 14. Chapter 7. Capillary electrophoresis and electrochromatography of carbohydrates - p. 311 15. Chapter 8. LC-MS/MS in glycomics and glycoproteomics analyses - p. 391 16. Chapter 9. Capillary electrophoresis-mass spectrometry of carbohydrates and glycoconjugates - p. 469 17. Chapter 10. Modern techniques for separation, mass spectrometric detection, and characterization of glycolipids - p. 509 18. Chapter 11. Liquid-phase separation methods hyphenated to electrospray ionization mass spectrometry for structural analysis of chondroitin/dermatan sulfate - p. 529 19. Chapter 12. CE/microchip electrophoresis of carbohydrates and glycoconjugates with electrochemical detection - p. 568 20. Chapter 13. CE and HPLC of carbohydrate species using contactless conductivity detection - p. 595 21. Chapter 14. Carbohydrate analysis by high-performance liquid chromatography (HPLC) with evaporative light-scattering detection (ELSD) - p. 631 22. Chapter 15. Capillary electrophoresis of glycoproteins - p. 645 23. Chapter 16. Capillary electrophoresis analysis of intact and depolymerized complex heteropolysaccharides for quality assurance and purity - p. 729 24. Chapter 17. HPLC- and CE-based methods for the characterization of therapeutic glycoproteins - p. 761 25. Chapter 18. Analysis of carbohydrates and glycoconjugates in food by CE and HPLC - p. 815 26. Database search assisted N-glycan structure identification - p. 843 27. Subject Index - p. 859 28. Author Index - p. 875 29. A - p. 875 30. B - p. 877 31. C - p. 880 32. D - p. 885 33. E - p. 888 34. F - p. 889 35. G - p. 891 36. H - p. 894 37. I - p. 898 38. J - p. 898 39. K - p. 900 40. L - p. 904 41. M - p. 908 42. N - p. 913 43. O - p. 914 44. P - p. 915 45. Q - p. 918 46. R - p. 919 47. S - p. 921 48. T - p. 927 49. U - p. 930 50. V - p. 930 51. W - p. 932 52. X - p. 934 53. Y - p. 935 54. Z - p. 936
Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges.
This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques.
1. Title of Book - p. Cover 2. Cover image - p. Cover 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Contributors - p. xi 7. Preface - p. xv 8. Chapter 1. Derivatization of carbohydrates for analysis by liquid chromatography and capillary electrophoresis - p. 6 9. Chapter 2. Reversed-phase and hydrophobic interaction chromatography of carbohydrates and glycoconjugates - p. 35 10. Chapter 3. HPLC of carbohydrates with cation-exchange silica and resin-based stationary phases - p. 146 11. Chapter 4. High-performance anion-exchange chromatography with pulsed amperometric detection for carbohydrate and glycoconjugate analyses - p. 157 12. Chapter 5. Hydrophilic interaction liquid chromatography-mass spectrometry for the characterization of glycoproteins at the glycan, peptide, subunit, and intact level - p. 218 13. Chapter 6. Frontal affinity chromatography: A unique approach for weak interaction analysis targeting lectins and oligosaccharides - p. 279 14. Chapter 7. Capillary electrophoresis and electrochromatography of carbohydrates - p. 311 15. Chapter 8. LC-MS/MS in glycomics and glycoproteomics analyses - p. 391 16. Chapter 9. Capillary electrophoresis-mass spectrometry of carbohydrates and glycoconjugates - p. 469 17. Chapter 10. Modern techniques for separation, mass spectrometric detection, and characterization of glycolipids - p. 509 18. Chapter 11. Liquid-phase separation methods hyphenated to electrospray ionization mass spectrometry for structural analysis of chondroitin/dermatan sulfate - p. 529 19. Chapter 12. CE/microchip electrophoresis of carbohydrates and glycoconjugates with electrochemical detection - p. 568 20. Chapter 13. CE and HPLC of carbohydrate species using contactless conductivity detection - p. 595 21. Chapter 14. Carbohydrate analysis by high-performance liquid chromatography (HPLC) with evaporative light-scattering detection (ELSD) - p. 631 22. Chapter 15. Capillary electrophoresis of glycoproteins - p. 645 23. Chapter 16. Capillary electrophoresis analysis of intact and depolymerized complex heteropolysaccharides for quality assurance and purity - p. 729 24. Chapter 17. HPLC- and CE-based methods for the characterization of therapeutic glycoproteins - p. 761 25. Chapter 18. Analysis of carbohydrates and glycoconjugates in food by CE and HPLC - p. 815 26. Database search assisted N-glycan structure identification - p. 843 27. Subject Index - p. 859 28. Author Index - p. 875 29. A - p. 875 30. B - p. 877 31. C - p. 880 32. D - p. 885 33. E - p. 888 34. F - p. 889 35. G - p. 891 36. H - p. 894 37. I - p. 898 38. J - p. 898 39. K - p. 900 40. L - p. 904 41. M - p. 908 42. N - p. 913 43. O - p. 914 44. P - p. 915 45. Q - p. 918 46. R - p. 919 47. S - p. 921 48. T - p. 927 49. U - p. 930 50. V - p. 930 51. W - p. 932 52. X - p. 934 53. Y - p. 935 54. Z - p. 936
