carboxylases series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Purification and characterization of mitochondrial biotin-dependent carboxylases from native tissues - p. 2 9. Chapter Two: Sample optimizations to enable the structure determination of biotin-dependent carboxylases - p. 32 10. Chapter Three: Purification of heteromeric acetyl-CoA carboxylases from Escherichia coli for structure solution - p. 46 11. Chapter Four: Insights into the methodology of acetyl-CoA carboxylase inhibition - p. 69 12. Chapter Five: Characterization of guanidine carboxylases - p. 106 13. Chapter Six: Methods to study prFMN-UbiD mediated (de)carboxylation - p. 127 14. Chapter Seven: Enzyme cascades for in vitro and in vivo FMN prenylation and UbiD (de)carboxylase activation under aerobic conditions - p. 152 15. Chapter Eight: Cellular and biochemical approaches to define GGCX carboxylation of vitamin K-dependent proteins - p. 176 16. Chapter Nine: Assessment of gamma-glutamyl carboxylase activity in its native milieu - p. 208 17. Chapter Ten: Expression, purification, and activation of one key enzyme in anaerobic CO2 fixation: Carbon monoxide dehydrogenase II from Carboxydothermus hydrogenoformans - p. 238 18. Chapter Eleven: Molybdenum-containing CO dehydrogenase and formate dehydrogenases - p. 258 19. Chapter Twelve: Assessing the role of redox carriers in the reduction of CO2 by the oxo-acid: ferredoxin oxidoreductase superfamily - p. 276 20. Chapter Thirteen: Measuring carbonic anhydrase activity in alpha-carboxysomes using stopped-flow - p. 298 21. Chapter Fourteen: Radiometric determination of rubisco activation state and quantity in leaves - p. 324 22. Chapter Fifteen: Computational methods for the study of carboxylases: The case of crotonyl-CoA carboxylase/reductase - p. 354 23. Chapter Sixteen: Carboxylation in de novo purine biosynthesis - p. 390
carboxylases series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter One: Purification and characterization of mitochondrial biotin-dependent carboxylases from native tissues - p. 2 9. Chapter Two: Sample optimizations to enable the structure determination of biotin-dependent carboxylases - p. 32 10. Chapter Three: Purification of heteromeric acetyl-CoA carboxylases from Escherichia coli for structure solution - p. 46 11. Chapter Four: Insights into the methodology of acetyl-CoA carboxylase inhibition - p. 69 12. Chapter Five: Characterization of guanidine carboxylases - p. 106 13. Chapter Six: Methods to study prFMN-UbiD mediated (de)carboxylation - p. 127 14. Chapter Seven: Enzyme cascades for in vitro and in vivo FMN prenylation and UbiD (de)carboxylase activation under aerobic conditions - p. 152 15. Chapter Eight: Cellular and biochemical approaches to define GGCX carboxylation of vitamin K-dependent proteins - p. 176 16. Chapter Nine: Assessment of gamma-glutamyl carboxylase activity in its native milieu - p. 208 17. Chapter Ten: Expression, purification, and activation of one key enzyme in anaerobic CO2 fixation: Carbon monoxide dehydrogenase II from Carboxydothermus hydrogenoformans - p. 238 18. Chapter Eleven: Molybdenum-containing CO dehydrogenase and formate dehydrogenases - p. 258 19. Chapter Twelve: Assessing the role of redox carriers in the reduction of CO2 by the oxo-acid: ferredoxin oxidoreductase superfamily - p. 276 20. Chapter Thirteen: Measuring carbonic anhydrase activity in alpha-carboxysomes using stopped-flow - p. 298 21. Chapter Fourteen: Radiometric determination of rubisco activation state and quantity in leaves - p. 324 22. Chapter Fifteen: Computational methods for the study of carboxylases: The case of crotonyl-CoA carboxylase/reductase - p. 354 23. Chapter Sixteen: Carboxylation in de novo purine biosynthesis - p. 390
