Carotenoids: Biological Functions of Carotenoids and Apocarotenoids in Natural and Artificial Systems, Volume 674 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Ultrafast laser spectroscopic studies on carotenoids in solution and on those bound to photosynthetic pigment-protein complexes, Assessing photoprotective functions of carotenoids in photosynthetic systems of plants and green algae, Fluorescence of carotenoids: probing binding site interactions and conformational motion in carotenoproteins, Resonance Raman: A powerful tool to interrogate carotenoids in biological matrices, and much more.
Other chapters in the book cover Engineering the carotenoid biosynthetic pathway to study the function of carotenoids in light-harvesting complexes, Carotenoids as proxies for variations in photosynthesis and phenology in response to environmental and climatic change, Apocarotenoid pigment biosynthesis in non-model plants, Apocarotenoid transport in plants, Screening for apocarotenoid plant growth regulators in Arabidopsis, Effects of herbivory on carotenoid biosynthesis and breakdown, Biosynthesis and action of apocarotenoid plant hormones, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xvii 7. Chapter One: Ultrafast laser spectroscopic studies on carotenoids in solution and on those bound to photosynthetic pigment-protein complexes - p. 1 8. Chapter Two: Assessing photoprotective functions of carotenoids in photosynthetic systems of plants and green algae - p. 53 9. Chapter Three: Fluorescence of carotenoids: Probing binding site interactions and conformational motion in carotenoproteins - p. 85 10. Chapter Four: Resonance Raman: A powerful tool to interrogate carotenoids in biological matrices - p. 113 11. Chapter Five: Engineering purple bacterial carotenoid biosynthesis to study the roles of carotenoids in light-harvesting complexes - p. 137 12. Chapter Six: Extraction, detection, and imaging of the macular carotenoids - p. 185 13. Chapter Seven: Analysis of macular carotenoids in the developing macaque retina: The timeline of macular pigment development - p. 229 14. Chapter Eight: Assessment of dietary carotenoid intake and biologic measurement of exposure in humans - p. 261 15. Chapter Nine: A guide for the evaluation of in vitro bioaccessibility of carotenoids - p. 300 16. Chapter Ten: A fast and simplified method to estimate bioaccessibility of carotenoids from plant tissues - p. 329 17. Chapter Eleven: Microsomal triglyceride transfer protein-mediated transfer of β-carotene from donor to acceptor vesicles in vitro - p. 343 18. Chapter Twelve: Development and validation of a method to deliver vitamin A to macrophages - p. 363 19. Chapter Thirteen: Methods for assessing the interaction of apocarotenoids with vertebrate nuclear receptors - p. 391 20. Chapter Fourteen: Carotenoid modifying enzymes in metazoans - p. 405 21. Chapter Fifteen: Expression and biochemical analyses of proteins involved in the transport of carotenoids and retinoids - p. 447 22. Chapter Sixteen: Screening for apocarotenoid plant growth regulators in Arabidopsis - p. 481 23. Chapter Seventeen: Effects of herbivory on carotenoid biosynthesis and breakdown - p. 497 24. Chapter Eighteen: Strigolactone signaling complex formation in yeast: A paradigm for studying hormone-induced receptor interaction with multiple downstream proteins - p. 519
Carotenoids: Biological Functions of Carotenoids and Apocarotenoids in Natural and Artificial Systems, Volume 674 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Ultrafast laser spectroscopic studies on carotenoids in solution and on those bound to photosynthetic pigment-protein complexes, Assessing photoprotective functions of carotenoids in photosynthetic systems of plants and green algae, Fluorescence of carotenoids: probing binding site interactions and conformational motion in carotenoproteins, Resonance Raman: A powerful tool to interrogate carotenoids in biological matrices, and much more.
Other chapters in the book cover Engineering the carotenoid biosynthetic pathway to study the function of carotenoids in light-harvesting complexes, Carotenoids as proxies for variations in photosynthesis and phenology in response to environmental and climatic change, Apocarotenoid pigment biosynthesis in non-model plants, Apocarotenoid transport in plants, Screening for apocarotenoid plant growth regulators in Arabidopsis, Effects of herbivory on carotenoid biosynthesis and breakdown, Biosynthesis and action of apocarotenoid plant hormones, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xvii 7. Chapter One: Ultrafast laser spectroscopic studies on carotenoids in solution and on those bound to photosynthetic pigment-protein complexes - p. 1 8. Chapter Two: Assessing photoprotective functions of carotenoids in photosynthetic systems of plants and green algae - p. 53 9. Chapter Three: Fluorescence of carotenoids: Probing binding site interactions and conformational motion in carotenoproteins - p. 85 10. Chapter Four: Resonance Raman: A powerful tool to interrogate carotenoids in biological matrices - p. 113 11. Chapter Five: Engineering purple bacterial carotenoid biosynthesis to study the roles of carotenoids in light-harvesting complexes - p. 137 12. Chapter Six: Extraction, detection, and imaging of the macular carotenoids - p. 185 13. Chapter Seven: Analysis of macular carotenoids in the developing macaque retina: The timeline of macular pigment development - p. 229 14. Chapter Eight: Assessment of dietary carotenoid intake and biologic measurement of exposure in humans - p. 261 15. Chapter Nine: A guide for the evaluation of in vitro bioaccessibility of carotenoids - p. 300 16. Chapter Ten: A fast and simplified method to estimate bioaccessibility of carotenoids from plant tissues - p. 329 17. Chapter Eleven: Microsomal triglyceride transfer protein-mediated transfer of β-carotene from donor to acceptor vesicles in vitro - p. 343 18. Chapter Twelve: Development and validation of a method to deliver vitamin A to macrophages - p. 363 19. Chapter Thirteen: Methods for assessing the interaction of apocarotenoids with vertebrate nuclear receptors - p. 391 20. Chapter Fourteen: Carotenoid modifying enzymes in metazoans - p. 405 21. Chapter Fifteen: Expression and biochemical analyses of proteins involved in the transport of carotenoids and retinoids - p. 447 22. Chapter Sixteen: Screening for apocarotenoid plant growth regulators in Arabidopsis - p. 481 23. Chapter Seventeen: Effects of herbivory on carotenoid biosynthesis and breakdown - p. 497 24. Chapter Eighteen: Strigolactone signaling complex formation in yeast: A paradigm for studying hormone-induced receptor interaction with multiple downstream proteins - p. 519
