Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology, Volume 671, the latest release in the Methods of Enzymology series highlights new advances in the field with chapters on Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches, Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes, Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids, Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages, and much more.
Additional chapters in this new release cover Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis, The isolation of sub-chromoplast structures from tomato and capsicum fruit, Carrot protoplasts as a suitable method for protein subcellular localization, High throughput production and characterization of carotenoid enzymes for structural and functional studies, Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation, and more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Illustrations - p. iii 5. List of Tables - p. iii 6. Series Page - p. iii 7. Copyright - p. iii 8. Contributors - p. iii 9. Preface - p. iii 10. Chapter One: Golden Rice—Lessons learned for inspiring future metabolic engineering strategies and synthetic biology solutions - p. iii 11. Chapter Two: Metabolic engineering of cassava to improve carotenoids - p. iii 12. Chapter Three: Elevating fruit carotenoid content in apple (Malus x domestica Borkh) - p. iii 13. Chapter Four: The breeder's tool-box for enhancing the content of esterified carotenoids in wheat: From extraction and profiling of carotenoids to marker-assisted selection of candidate genes - p. iii 14. Chapter Five: Understanding carotenoid biosynthetic pathway control points using metabolomic analysis and natural genetic variation - p. iii 15. Chapter Six: Enzymatic isomerization of ζ-carotene mediated by the heme-containing isomerase Z-ISO - p. iii 16. Chapter Seven: Genomics-based strategies toward the identification of a Z-ISO carotenoid biosynthetic enzyme suitable for structural studies - p. iii 17. Chapter Eight: Metalloenzymes involved in carotenoid biosynthesis in plants - p. iii 18. Chapter Nine: Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation - p. iii 19. Chapter Ten: Preparation of carotenoid cleavage dioxygenases for X-ray crystallography - p. iii 20. Chapter Eleven: Carrot protoplasts as a suitable method for protein subcellular localization - p. iii 21. Chapter Twelve: Isolation and characterization of sub-plastidial fractions from carotenoid rich fruits - p. iii 22. Chapter Thirteen: Protein–protein interaction techniques to investigate post-translational regulation of carotenogenesis - p. iii 23. Chapter Fourteen: Tracking subplastidic localization of carotenoid metabolic enzymes with proteomics - p. iii 24. Chapter Fifteen: Use of directed enzyme evolution to create novel biosynthetic pathways for production of rare or non-natural carotenoids - p. iii 25. Chapter Sixteen: A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: Read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi - p. iii 26. Chapter Seventeen: Imaging retinaldehyde-protein binding in plants using a merocyanine reporter - p. iii 27. Chapter Eighteen: Synthesis and characterization of abscisic acid receptor modulators - p. iii 28. Chapter Nineteen: Heterologous complementation in bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes - p. iii 29. Chapter Twenty: An in vivo plant platform to assess genes encoding native and synthetic enzymes for carotenoid biosynthesis - p. iii 30. Chapter Twenty-One: Rice callus as a high-throughput platform for synthetic biology and metabolic engineering of carotenoids - p. iii 31. Chapter Twenty-Two: Characterizing cytochrome P450 enzymes involved in plant apocarotenoid metabolism by using an engineered yeast system - p. iii
Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology, Volume 671, the latest release in the Methods of Enzymology series highlights new advances in the field with chapters on Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches, Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes, Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids, Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages, and much more.
Additional chapters in this new release cover Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis, The isolation of sub-chromoplast structures from tomato and capsicum fruit, Carrot protoplasts as a suitable method for protein subcellular localization, High throughput production and characterization of carotenoid enzymes for structural and functional studies, Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation, and more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Illustrations - p. iii 5. List of Tables - p. iii 6. Series Page - p. iii 7. Copyright - p. iii 8. Contributors - p. iii 9. Preface - p. iii 10. Chapter One: Golden Rice—Lessons learned for inspiring future metabolic engineering strategies and synthetic biology solutions - p. iii 11. Chapter Two: Metabolic engineering of cassava to improve carotenoids - p. iii 12. Chapter Three: Elevating fruit carotenoid content in apple (Malus x domestica Borkh) - p. iii 13. Chapter Four: The breeder's tool-box for enhancing the content of esterified carotenoids in wheat: From extraction and profiling of carotenoids to marker-assisted selection of candidate genes - p. iii 14. Chapter Five: Understanding carotenoid biosynthetic pathway control points using metabolomic analysis and natural genetic variation - p. iii 15. Chapter Six: Enzymatic isomerization of ζ-carotene mediated by the heme-containing isomerase Z-ISO - p. iii 16. Chapter Seven: Genomics-based strategies toward the identification of a Z-ISO carotenoid biosynthetic enzyme suitable for structural studies - p. iii 17. Chapter Eight: Metalloenzymes involved in carotenoid biosynthesis in plants - p. iii 18. Chapter Nine: Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation - p. iii 19. Chapter Ten: Preparation of carotenoid cleavage dioxygenases for X-ray crystallography - p. iii 20. Chapter Eleven: Carrot protoplasts as a suitable method for protein subcellular localization - p. iii 21. Chapter Twelve: Isolation and characterization of sub-plastidial fractions from carotenoid rich fruits - p. iii 22. Chapter Thirteen: Protein–protein interaction techniques to investigate post-translational regulation of carotenogenesis - p. iii 23. Chapter Fourteen: Tracking subplastidic localization of carotenoid metabolic enzymes with proteomics - p. iii 24. Chapter Fifteen: Use of directed enzyme evolution to create novel biosynthetic pathways for production of rare or non-natural carotenoids - p. iii 25. Chapter Sixteen: A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: Read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi - p. iii 26. Chapter Seventeen: Imaging retinaldehyde-protein binding in plants using a merocyanine reporter - p. iii 27. Chapter Eighteen: Synthesis and characterization of abscisic acid receptor modulators - p. iii 28. Chapter Nineteen: Heterologous complementation in bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes - p. iii 29. Chapter Twenty: An in vivo plant platform to assess genes encoding native and synthetic enzymes for carotenoid biosynthesis - p. iii 30. Chapter Twenty-One: Rice callus as a high-throughput platform for synthetic biology and metabolic engineering of carotenoids - p. iii 31. Chapter Twenty-Two: Characterizing cytochrome P450 enzymes involved in plant apocarotenoid metabolism by using an engineered yeast system - p. iii
