Cell Death, Part A, Volume 205 explores the diverse mechanisms and methodologies underlying cell death, providing an up-to-date resource for researchers and clinicians in the biomedical sciences. Chapters in this new release include Methodologies for Monitoring Alkaliptosis in Cancer Cells, Protein-complementation assay and DISC analysis, Detecting phosphorylated MLKL in murine tissues, Establishment of different cell death models (Apoptosis, Necroptosis, Pyroptosis), Implementation of the FENIX assay to determine anti-ferroptotic properties of test compounds, CRISPR-Cas9 knockout screens to identify drug resistance genes in Acute Myeloid Leukemia, and much more.
Additional chapters cover Cell death assays for the detection of acute kidney tubular necrosis, Methods of cell death imaging by Incucyte and similar platforms, Super-resolution microscopy-based assessment of organellar redox-active iron distribution and its relevance for ferroptosis, Monitoring inflammasome activation by analyzing ASC specks and oligomerization, LPS-induced sepsis/ and TNF-induced SIRS (In vivo cell death models), Evaluating protease activation and inactivation of gasdermin cell death effectors, and Measurement of plasma membrane rupture via confocal microscopy.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: Protein-fragment complementation assays to analyze the CD95 complex - p. 1 9. Chapter Two: Monitoring inflammasome activation by analysing ASC specks and oligomerisation - p. 19 10. Chapter Three: Assessing the cleavage of gasdermin-family cell death effectors by microbial proteases - p. 37 11. Chapter Four: Measurement of plasma membrane rupture via confocal microscopy - p. 47 12. Chapter Five: Implementation of the FENIX assay to assess the anti-ferroptotic properties of test compounds - p. 63 13. Chapter Six: Super resolution microscopy-based assessment of organellar redox-active iron transfer and its relevance for ferroptosis - p. 85 14. Chapter Seven: Methodologies for monitoring alkaliptosis in cancer cells - p. 107 15. Chapter Eight: Cell death assays for the detection of acute kidney tubular necrosis - p. 125 16. Chapter Nine: Single cell temporally-resolved analysis of apoptotic and non-apoptotic cell death by live-cell imaging - p. 141 17. Chapter Ten: Detecting phosphorylated MLKL in murine tissues - p. 159 18. Chapter Eleven: Methods for the detection of canonical markers of apoptosis, necroptosis and pyroptosis - p. 171 19. Chapter Twelve: Establishment of LPS-induced sepsis and TNF-induced SIRS - p. 189 20. Chapter Thirteen: CRISPR-Cas9 knockout screens to identify drug resistance genes in acute myeloid leukemia - p. 199
Cell Death, Part A, Volume 205 explores the diverse mechanisms and methodologies underlying cell death, providing an up-to-date resource for researchers and clinicians in the biomedical sciences. Chapters in this new release include Methodologies for Monitoring Alkaliptosis in Cancer Cells, Protein-complementation assay and DISC analysis, Detecting phosphorylated MLKL in murine tissues, Establishment of different cell death models (Apoptosis, Necroptosis, Pyroptosis), Implementation of the FENIX assay to determine anti-ferroptotic properties of test compounds, CRISPR-Cas9 knockout screens to identify drug resistance genes in Acute Myeloid Leukemia, and much more.
Additional chapters cover Cell death assays for the detection of acute kidney tubular necrosis, Methods of cell death imaging by Incucyte and similar platforms, Super-resolution microscopy-based assessment of organellar redox-active iron distribution and its relevance for ferroptosis, Monitoring inflammasome activation by analyzing ASC specks and oligomerization, LPS-induced sepsis/ and TNF-induced SIRS (In vivo cell death models), Evaluating protease activation and inactivation of gasdermin cell death effectors, and Measurement of plasma membrane rupture via confocal microscopy.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: Protein-fragment complementation assays to analyze the CD95 complex - p. 1 9. Chapter Two: Monitoring inflammasome activation by analysing ASC specks and oligomerisation - p. 19 10. Chapter Three: Assessing the cleavage of gasdermin-family cell death effectors by microbial proteases - p. 37 11. Chapter Four: Measurement of plasma membrane rupture via confocal microscopy - p. 47 12. Chapter Five: Implementation of the FENIX assay to assess the anti-ferroptotic properties of test compounds - p. 63 13. Chapter Six: Super resolution microscopy-based assessment of organellar redox-active iron transfer and its relevance for ferroptosis - p. 85 14. Chapter Seven: Methodologies for monitoring alkaliptosis in cancer cells - p. 107 15. Chapter Eight: Cell death assays for the detection of acute kidney tubular necrosis - p. 125 16. Chapter Nine: Single cell temporally-resolved analysis of apoptotic and non-apoptotic cell death by live-cell imaging - p. 141 17. Chapter Ten: Detecting phosphorylated MLKL in murine tissues - p. 159 18. Chapter Eleven: Methods for the detection of canonical markers of apoptosis, necroptosis and pyroptosis - p. 171 19. Chapter Twelve: Establishment of LPS-induced sepsis and TNF-induced SIRS - p. 189 20. Chapter Thirteen: CRISPR-Cas9 knockout screens to identify drug resistance genes in acute myeloid leukemia - p. 199
