Cell Death, Part D, Volume 209 explores the latest advances in the mechanisms, regulation, and significance of cell death in health and disease. Covering diverse topics such as apoptosis, necrosis, autophagy, and emerging pathways, this volume presents chapters on Utilizing FLAMBE to study the structure-function relationship and regulation of monomeric BAX activation in solution, Sensitive detection of Lysosomal Membrane Permeabilization using the Galectin Puncta Assay, Assessing the activity of caspase-activated pannexin-1 channels by flow cytometry, In vitro culture of intestinal organoids for cell death assessments, Optimized Strategies for Necroptosis Induction and Evaluation, and more.
Additional sections cover Ferroptosis induction via genetic approaches - CRISPR/Cas9-based disruption on key anti-ferroptotic genes, Reprogramming T cell fate through antibody-mediated galectin-1 blockade, 3D bioluminescence imaging assays for measuring in vitro CAR T cell cytotoxicity, The heat-ramp method to study regulated cell death in the pathogenic yeast Cryptococcus neoformans, High throughput assessment of cellular viability, and High-Throughput Screening for Cell Death Inducers and Inhibitors. This book will serve as a valuable resource for researchers, clinicians, and students interested in understanding cellular processes and their implications for therapy and diagnostics.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xi 8. Chapter One: Utilizing FLAMBE to study the structure-function relationship and regulation of monomeric BAX activation in solution - p. 1 9. Chapter Two: Sensitive detection of lysosomal membrane permeabilization using the galectin puncta assay - p. 27 10. Chapter Three: Assessing the activity of caspase-activated pannexin-1 channels by flow cytometry - p. 41 11. Chapter Four: In vitro culture of intestinal organoids for cell death assessments - p. 55 12. Chapter Five: Optimized strategies for necroptosis induction and evaluation - p. 67 13. Chapter Six: Ferroptosis induction via genetic approaches - CRISPR/Cas9-based disruption on key anti-ferroptotic genes - p. 91 14. Chapter Seven: Reprogramming T cell fate through antibody-mediated galectin-1 blockade - p. 105 15. Chapter Eight: 3D bioluminescence imaging assays for measuring in vitro CAR T cell cytotoxicity - p. 123 16. Chapter Nine: The heat-ramp method to study regulated cell death in a pathogenic yeast Cryptococcus neoformans - p. 141 17. Chapter Ten: High throughput assessment of viability in 2D and 3D cell culture - p. 159 18. Chapter Eleven: High-throughput screening for cell death inducers and inhibitors - p. 173
Cell Death, Part D, Volume 209 explores the latest advances in the mechanisms, regulation, and significance of cell death in health and disease. Covering diverse topics such as apoptosis, necrosis, autophagy, and emerging pathways, this volume presents chapters on Utilizing FLAMBE to study the structure-function relationship and regulation of monomeric BAX activation in solution, Sensitive detection of Lysosomal Membrane Permeabilization using the Galectin Puncta Assay, Assessing the activity of caspase-activated pannexin-1 channels by flow cytometry, In vitro culture of intestinal organoids for cell death assessments, Optimized Strategies for Necroptosis Induction and Evaluation, and more.
Additional sections cover Ferroptosis induction via genetic approaches - CRISPR/Cas9-based disruption on key anti-ferroptotic genes, Reprogramming T cell fate through antibody-mediated galectin-1 blockade, 3D bioluminescence imaging assays for measuring in vitro CAR T cell cytotoxicity, The heat-ramp method to study regulated cell death in the pathogenic yeast Cryptococcus neoformans, High throughput assessment of cellular viability, and High-Throughput Screening for Cell Death Inducers and Inhibitors. This book will serve as a valuable resource for researchers, clinicians, and students interested in understanding cellular processes and their implications for therapy and diagnostics.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xi 8. Chapter One: Utilizing FLAMBE to study the structure-function relationship and regulation of monomeric BAX activation in solution - p. 1 9. Chapter Two: Sensitive detection of lysosomal membrane permeabilization using the galectin puncta assay - p. 27 10. Chapter Three: Assessing the activity of caspase-activated pannexin-1 channels by flow cytometry - p. 41 11. Chapter Four: In vitro culture of intestinal organoids for cell death assessments - p. 55 12. Chapter Five: Optimized strategies for necroptosis induction and evaluation - p. 67 13. Chapter Six: Ferroptosis induction via genetic approaches - CRISPR/Cas9-based disruption on key anti-ferroptotic genes - p. 91 14. Chapter Seven: Reprogramming T cell fate through antibody-mediated galectin-1 blockade - p. 105 15. Chapter Eight: 3D bioluminescence imaging assays for measuring in vitro CAR T cell cytotoxicity - p. 123 16. Chapter Nine: The heat-ramp method to study regulated cell death in a pathogenic yeast Cryptococcus neoformans - p. 141 17. Chapter Ten: High throughput assessment of viability in 2D and 3D cell culture - p. 159 18. Chapter Eleven: High-throughput screening for cell death inducers and inhibitors - p. 173
