Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_ix 6. Preface - p. page_xiii 7. Chapter One: Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein - p. page_xiii 8. Chapter Two: (Strept)avidin as a template for ligands other than biotin: An overview - p. page_20 9. Chapter Three: Engineering siderophores - p. page_28 10. Chapter Four: Competitive profiling for enzyme inhibitors using chemical probes - p. page_47 11. Chapter Five: The NMR tube bioreactor - p. page_69 12. Chapter Six: A chemically-controlled system for activating RAS GTPases - p. page_101 13. Chapter Seven: Temporal and rheostatic control of genome editing with a chemically-inducible Cas9 - p. page_117 14. Chapter Eight: Synthetic receptors to understand and control cellular functions - p. page_141 15. Chapter Nine: A suite of bioassays to evaluate CREB inhibitors - p. page_167 16. Chapter Ten: Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe - p. page_184 17. Chapter Eleven: REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteins - p. page_201 18. Chapter Twelve: Building artificial genetic circuits to understand protein function - p. page_230 19. Chapter Thirteen: Methods for studying human sirtuins with activity-based chemical probes - p. page_250 20. Chapter Fourteen: Site-directed labeling of β-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors - p. page_269 21. Chapter Fifteen: Reversible biotinylation of purified proteins for measuring protein–protein interactions - p. page_280
Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_ix 6. Preface - p. page_xiii 7. Chapter One: Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein - p. page_xiii 8. Chapter Two: (Strept)avidin as a template for ligands other than biotin: An overview - p. page_20 9. Chapter Three: Engineering siderophores - p. page_28 10. Chapter Four: Competitive profiling for enzyme inhibitors using chemical probes - p. page_47 11. Chapter Five: The NMR tube bioreactor - p. page_69 12. Chapter Six: A chemically-controlled system for activating RAS GTPases - p. page_101 13. Chapter Seven: Temporal and rheostatic control of genome editing with a chemically-inducible Cas9 - p. page_117 14. Chapter Eight: Synthetic receptors to understand and control cellular functions - p. page_141 15. Chapter Nine: A suite of bioassays to evaluate CREB inhibitors - p. page_167 16. Chapter Ten: Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe - p. page_184 17. Chapter Eleven: REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteins - p. page_201 18. Chapter Twelve: Building artificial genetic circuits to understand protein function - p. page_230 19. Chapter Thirteen: Methods for studying human sirtuins with activity-based chemical probes - p. page_250 20. Chapter Fourteen: Site-directed labeling of β-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors - p. page_269 21. Chapter Fifteen: Reversible biotinylation of purified proteins for measuring protein–protein interactions - p. page_280
