Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Preface - p. page_xv 7. Chapter One: Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates - p. page_xvii 8. Chapter Two: Chemical tools for selective activity profiling of bacterial penicillin-binding proteins - p. page_26 9. Chapter Three: Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking - p. page_55 10. Chapter Four: Gram-scale preparation of the antibiotic lead compound salicyl-AMS, a potent inhibitor of bacterial salicylate adenylation enzymes - p. page_67 11. Chapter Five: Probe-enabled approaches for function-dependent cell sorting and characterization of microbiome subpopulations - p. page_87 12. Chapter Six: Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity - p. page_107 13. Chapter Seven: Controlled release of bioactive signaling molecules - p. page_127 14. Chapter Eight: Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation - p. page_138 15. Chapter Nine: Engineering reversible cell-cell interactions with chemical biology - p. page_165 16. Chapter Ten: Fast phosphine-activated control of protein function using unnatural lysine analogues - p. page_190 17. Chapter Eleven: Photopharmacological control of lipid function - p. page_217 18. Chapter Twelve: Expanding the substrate selectivity of SNAP/CLIP-tagging of intracellular targets - p. page_232 19. Chapter Thirteen: Light-induced protein proximity by activation of gibberellic acid derivatives in living cells - p. page_257 20. Chapter Fourteen: Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processes - p. page_271 21. Chapter Fifteen: Site-specific antibody fragment conjugates for targeted imaging - p. page_294 22. Chapter Sixteen: Quantifying protein-protein interactions of the acyl carrier protein with solvatochromic probes - p. page_320
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Preface - p. page_xv 7. Chapter One: Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates - p. page_xvii 8. Chapter Two: Chemical tools for selective activity profiling of bacterial penicillin-binding proteins - p. page_26 9. Chapter Three: Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking - p. page_55 10. Chapter Four: Gram-scale preparation of the antibiotic lead compound salicyl-AMS, a potent inhibitor of bacterial salicylate adenylation enzymes - p. page_67 11. Chapter Five: Probe-enabled approaches for function-dependent cell sorting and characterization of microbiome subpopulations - p. page_87 12. Chapter Six: Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity - p. page_107 13. Chapter Seven: Controlled release of bioactive signaling molecules - p. page_127 14. Chapter Eight: Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation - p. page_138 15. Chapter Nine: Engineering reversible cell-cell interactions with chemical biology - p. page_165 16. Chapter Ten: Fast phosphine-activated control of protein function using unnatural lysine analogues - p. page_190 17. Chapter Eleven: Photopharmacological control of lipid function - p. page_217 18. Chapter Twelve: Expanding the substrate selectivity of SNAP/CLIP-tagging of intracellular targets - p. page_232 19. Chapter Thirteen: Light-induced protein proximity by activation of gibberellic acid derivatives in living cells - p. page_257 20. Chapter Fourteen: Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processes - p. page_271 21. Chapter Fifteen: Site-specific antibody fragment conjugates for targeted imaging - p. page_294 22. Chapter Sixteen: Quantifying protein-protein interactions of the acyl carrier protein with solvatochromic probes - p. page_320
