Cilia: From Mechanisms to Disease, Part A, Volume 175 in the Methods in Cell Biology series, offers a range of techniques and protocols that can be used to study aspects of this interesting cellular organelle both in vitro and in vivo. Sections in this new release include Protocols to induce and study ciliogenesis, Flow Cytometry-Based Approach for the study of primary Cilia, Microscopic observation of human airway ciliary movement using wheat germ agglutinin, Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins, Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method, and much more.
Other sections cover Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish, Visualizing Multiciliated Cells in the Zebrafish, Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas, Using Paramecium as a Model for Ciliopathies, Using organoids to study cilia, Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model, and more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. List of Tables - p. iii 6. Copyright - p. iii 7. Contributors - p. iii 8. Cilia: From Mechanisms to Disease - Part A - p. iii 9. Protocols to induce and study ciliogenesis - p. iii 10. A flow cytometry-based approach for the study of primary cilia - p. iii 11. Microscopic observation of human airway ciliary movement using wheat germ agglutinin - p. iii 12. Time-lapse imaging of primary cilium behavior with physiological expression of fluorescent ciliary proteins - p. iii 13. Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method - p. iii 14. High-efficient CRISPR/Cas9-mediated gene targeting to establish cell models of ciliopathies - p. iii 15. Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish - p. iii 16. Visualizing multiciliated cells in the zebrafish - p. iii 17. Isolation of ciliary ectosomes and analysis of amidated peptide-mediated chemotaxis in Chlamydomonas - p. iii 18. Methods for Paramecium tetraurelia ciliary membrane protein identification and function - p. iii 19. Using mammary organoids to study cilia - p. iii 20. Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model - p. iii
Cilia: From Mechanisms to Disease, Part A, Volume 175 in the Methods in Cell Biology series, offers a range of techniques and protocols that can be used to study aspects of this interesting cellular organelle both in vitro and in vivo. Sections in this new release include Protocols to induce and study ciliogenesis, Flow Cytometry-Based Approach for the study of primary Cilia, Microscopic observation of human airway ciliary movement using wheat germ agglutinin, Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins, Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method, and much more.
Other sections cover Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish, Visualizing Multiciliated Cells in the Zebrafish, Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas, Using Paramecium as a Model for Ciliopathies, Using organoids to study cilia, Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model, and more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. List of Tables - p. iii 6. Copyright - p. iii 7. Contributors - p. iii 8. Cilia: From Mechanisms to Disease - Part A - p. iii 9. Protocols to induce and study ciliogenesis - p. iii 10. A flow cytometry-based approach for the study of primary cilia - p. iii 11. Microscopic observation of human airway ciliary movement using wheat germ agglutinin - p. iii 12. Time-lapse imaging of primary cilium behavior with physiological expression of fluorescent ciliary proteins - p. iii 13. Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method - p. iii 14. High-efficient CRISPR/Cas9-mediated gene targeting to establish cell models of ciliopathies - p. iii 15. Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish - p. iii 16. Visualizing multiciliated cells in the zebrafish - p. iii 17. Isolation of ciliary ectosomes and analysis of amidated peptide-mediated chemotaxis in Chlamydomonas - p. iii 18. Methods for Paramecium tetraurelia ciliary membrane protein identification and function - p. iii 19. Using mammary organoids to study cilia - p. iii 20. Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model - p. iii
