This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.
1. Cover image 2. Title page 3. Table of Contents 4. Methods in Enzymology 5. Copyright 6. Contributors 7. Preface 8. Series Page 9. Chapter One. Isolation of Intraflagellar Transport Particle Proteins from Chlamydomonas reinhardtii 10. Chapter Two. Molecular Tools for Studying the Radial Spoke 11. Chapter Three. Methods for Analysis of Calcium/Calmodulin Signaling in Cilia and Flagella 12. Chapter Four. Analysis of Flagellar Protein Ubiquitination 13. Chapter Five. Analysis of Ciliary Import 14. Chapter Six. Analysis of Signaling Pathways Controlling Flagellar Movements in Mammalian Spermatozoa 15. Chapter Seven. Analysis of Microtubule Plus-End-Tracking Proteins in Cilia 16. Chapter Eight. Biochemical and Physiological Analysis of Axonemal Dyneins 17. Chapter Nine. Measuring the Regulation of Dynein Activity During Flagellar Motility 18. Chapter Ten. Analysis of Interactions Between Intraflagellar Transport Proteins 19. Chapter Eleven. Analysis of Ciliary Membrane Protein Dynamics Using SNAP Technology 20. Chapter Twelve. Engineering Oscillating Microtubule Bundles 21. Chapter Thirteen. Purification of Exosome-Like Vesicles from Urine 22. Chapter Fourteen. Electron Microscopy of Flagella, Primary Cilia, and Intraflagellar Transport in Flat-Embedded Cells 23. Chapter Fifteen. In vivo Imaging of IFT in Chlamydomonas Flagella 24. Chapter Sixteen. Measuring Rates of Intraflagellar Transport Along Caenorhabditis elegans Sensory Cilia Using Fluorescence Microscopy 25. Chapter Seventeen. 3D Structural Analysis of Flagella/Cilia by Cryo-Electron Tomography 26. Chapter Eighteen. Electron Tomography of IFT Particles 27. Chapter Nineteen. Reconstitution of Flagellar Sliding 28. Author Index 29. Subject Index
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.
1. Cover image 2. Title page 3. Table of Contents 4. Methods in Enzymology 5. Copyright 6. Contributors 7. Preface 8. Series Page 9. Chapter One. Isolation of Intraflagellar Transport Particle Proteins from Chlamydomonas reinhardtii 10. Chapter Two. Molecular Tools for Studying the Radial Spoke 11. Chapter Three. Methods for Analysis of Calcium/Calmodulin Signaling in Cilia and Flagella 12. Chapter Four. Analysis of Flagellar Protein Ubiquitination 13. Chapter Five. Analysis of Ciliary Import 14. Chapter Six. Analysis of Signaling Pathways Controlling Flagellar Movements in Mammalian Spermatozoa 15. Chapter Seven. Analysis of Microtubule Plus-End-Tracking Proteins in Cilia 16. Chapter Eight. Biochemical and Physiological Analysis of Axonemal Dyneins 17. Chapter Nine. Measuring the Regulation of Dynein Activity During Flagellar Motility 18. Chapter Ten. Analysis of Interactions Between Intraflagellar Transport Proteins 19. Chapter Eleven. Analysis of Ciliary Membrane Protein Dynamics Using SNAP Technology 20. Chapter Twelve. Engineering Oscillating Microtubule Bundles 21. Chapter Thirteen. Purification of Exosome-Like Vesicles from Urine 22. Chapter Fourteen. Electron Microscopy of Flagella, Primary Cilia, and Intraflagellar Transport in Flat-Embedded Cells 23. Chapter Fifteen. In vivo Imaging of IFT in Chlamydomonas Flagella 24. Chapter Sixteen. Measuring Rates of Intraflagellar Transport Along Caenorhabditis elegans Sensory Cilia Using Fluorescence Microscopy 25. Chapter Seventeen. 3D Structural Analysis of Flagella/Cilia by Cryo-Electron Tomography 26. Chapter Eighteen. Electron Tomography of IFT Particles 27. Chapter Nineteen. Reconstitution of Flagellar Sliding 28. Author Index 29. Subject Index
