This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Contributors 6. Preface 7. Methods in Enzymology 8. Chapter One. Non-Motile Primary Cilia as Fluid Shear Stress Mechanosensors 9. Chapter Two. Biomechanical Measurement of Kinocilium 10. Chapter Three. Analysis of Primary Cilia in Directional Cell Migration in Fibroblasts 11. Chapter Four. Sperm Patch-Clamp 12. Chapter Five. Laser Trap Measurements of Flagellar Membrane Motility 13. Chapter Six. Probing Ciliogenesis Using Micropatterned Substrates 14. Chapter Seven. Fluorescent Imaging of Drosophila melanogaster Sperm in the Reproductive Tract 15. Chapter Eight. Visualization of Mouse Nodal Cilia and Nodal Flow 16. Chapter Nine. Dissecting the Functional Interplay Between the TOR Pathway and the Cilium in Zebrafish 17. Chapter Ten. Using Xenopus Skin to Study Cilia Development and Function 18. Chapter Eleven. Zebrafish Cilia 19. Chapter Twelve. Analysis of Ciliary Assembly and Function in Planaria 20. Chapter Thirteen. Discovery and Functional Evaluation of Ciliary Proteins in Tetrahymena thermophila 21. Chapter Fourteen. Analysis of Ciliogenesis in Primary Culture Mouse Tracheal Epithelial Cells 22. Chapter Fifteen. Isolation of Mammalian Primary Cilia 23. Chapter Sixteen. Finding Ciliary Genes 24. Chapter Seventeen. Chemical Screening Methods for Flagellar Phenotypes in Chlamydomonas 25. Chapter Eighteen. Centrosome Isolation and Analysis by Mass Spectrometry-Based Proteomics 26. Author Index 27. Subject Index
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Contributors 6. Preface 7. Methods in Enzymology 8. Chapter One. Non-Motile Primary Cilia as Fluid Shear Stress Mechanosensors 9. Chapter Two. Biomechanical Measurement of Kinocilium 10. Chapter Three. Analysis of Primary Cilia in Directional Cell Migration in Fibroblasts 11. Chapter Four. Sperm Patch-Clamp 12. Chapter Five. Laser Trap Measurements of Flagellar Membrane Motility 13. Chapter Six. Probing Ciliogenesis Using Micropatterned Substrates 14. Chapter Seven. Fluorescent Imaging of Drosophila melanogaster Sperm in the Reproductive Tract 15. Chapter Eight. Visualization of Mouse Nodal Cilia and Nodal Flow 16. Chapter Nine. Dissecting the Functional Interplay Between the TOR Pathway and the Cilium in Zebrafish 17. Chapter Ten. Using Xenopus Skin to Study Cilia Development and Function 18. Chapter Eleven. Zebrafish Cilia 19. Chapter Twelve. Analysis of Ciliary Assembly and Function in Planaria 20. Chapter Thirteen. Discovery and Functional Evaluation of Ciliary Proteins in Tetrahymena thermophila 21. Chapter Fourteen. Analysis of Ciliogenesis in Primary Culture Mouse Tracheal Epithelial Cells 22. Chapter Fifteen. Isolation of Mammalian Primary Cilia 23. Chapter Sixteen. Finding Ciliary Genes 24. Chapter Seventeen. Chemical Screening Methods for Flagellar Phenotypes in Chlamydomonas 25. Chapter Eighteen. Centrosome Isolation and Analysis by Mass Spectrometry-Based Proteomics 26. Author Index 27. Subject Index
