B12 Enzyme, Volume 667 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting timely, interesting chapters, including Part I: B12 Biosynthesis and B12 Chaperones, Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity, Biosynthesis of corrinoids, The human B12 trafficking chaperones: CblC, CblD and ATR, A method for the efficient adenosylation of corrinoids, Production, purification and liposome reconstitution of cobamide synthase, Extraction and Purification of Cobamides from Bacterial Cultures, Part II: The many faces of B12-dependent enzyme reactions, Methylcobalamin-dependent methionine synthase, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Section I: B12 Biosynthesis and B12 Chaperones - p. xviii 8. Chapter One: Biosynthesis of cobamides: Methods for the detection, analysis and production of cobamides and biosynthetic intermediates - p. 3 9. Chapter Two: Guardian of cobamide diversity: Probing the role of CobT in lower ligand activation in the biosynthesis of vitamin B12 and other cobamide cofactors - p. 54 10. Chapter Three: Purification and detection of vitamin B12 analogs - p. 61 11. Chapter Four: A method for the efficient adenosylation of corrinoids - p. 87 12. Chapter Five: A method for the production, purification and liposome reconstitution of cobamide synthase - p. 109 13. Chapter Six: A method for the isolation of α-ribazole from vitamin B12, and its enzymatic conversion to α-ribazole 5′-phosphate - p. 125 14. Chapter Seven: The human B12 trafficking chaperones: CblA, ATR, CblC and CblD - p. 137 15. Chapter Eight: Antivitamins B12: Synthesis and application as inhibitory ligand of the B12-tailoring enzyme CblC - p. 157 16. Section II: B12-dependent enzyme reactions - p. 178 17. Chapter Nine: Coenzyme B12-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase - p. 202 18. Chapter Ten: Reactivating chaperones for coenzyme B12-dependent diol and glycerol dehydratases and ethanolamine ammonia-lyase - p. 243 19. Chapter Eleven: Glutamate mutase and 2-methyleneglutarate mutase - p. 285 20. Chapter Twelve: Human B12-dependent enzymes: Methionine synthase and Methylmalonyl-CoA mutase - p. 309 21. Chapter Thirteen: Heterologous production and biophysical characterization of catabolic Nitratireductor pacificus pht-3B reductive dehalogenase - p. 327 22. Chapter Fourteen: An unusual light-sensing function for coenzyme B12 in bacterial transcription regulator CarH - p. 349
B12 Enzyme, Volume 667 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting timely, interesting chapters, including Part I: B12 Biosynthesis and B12 Chaperones, Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity, Biosynthesis of corrinoids, The human B12 trafficking chaperones: CblC, CblD and ATR, A method for the efficient adenosylation of corrinoids, Production, purification and liposome reconstitution of cobamide synthase, Extraction and Purification of Cobamides from Bacterial Cultures, Part II: The many faces of B12-dependent enzyme reactions, Methylcobalamin-dependent methionine synthase, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Section I: B12 Biosynthesis and B12 Chaperones - p. xviii 8. Chapter One: Biosynthesis of cobamides: Methods for the detection, analysis and production of cobamides and biosynthetic intermediates - p. 3 9. Chapter Two: Guardian of cobamide diversity: Probing the role of CobT in lower ligand activation in the biosynthesis of vitamin B12 and other cobamide cofactors - p. 54 10. Chapter Three: Purification and detection of vitamin B12 analogs - p. 61 11. Chapter Four: A method for the efficient adenosylation of corrinoids - p. 87 12. Chapter Five: A method for the production, purification and liposome reconstitution of cobamide synthase - p. 109 13. Chapter Six: A method for the isolation of α-ribazole from vitamin B12, and its enzymatic conversion to α-ribazole 5′-phosphate - p. 125 14. Chapter Seven: The human B12 trafficking chaperones: CblA, ATR, CblC and CblD - p. 137 15. Chapter Eight: Antivitamins B12: Synthesis and application as inhibitory ligand of the B12-tailoring enzyme CblC - p. 157 16. Section II: B12-dependent enzyme reactions - p. 178 17. Chapter Nine: Coenzyme B12-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase - p. 202 18. Chapter Ten: Reactivating chaperones for coenzyme B12-dependent diol and glycerol dehydratases and ethanolamine ammonia-lyase - p. 243 19. Chapter Eleven: Glutamate mutase and 2-methyleneglutarate mutase - p. 285 20. Chapter Twelve: Human B12-dependent enzymes: Methionine synthase and Methylmalonyl-CoA mutase - p. 309 21. Chapter Thirteen: Heterologous production and biophysical characterization of catabolic Nitratireductor pacificus pht-3B reductive dehalogenase - p. 327 22. Chapter Fourteen: An unusual light-sensing function for coenzyme B12 in bacterial transcription regulator CarH - p. 349
