CRISPR-Cas Systems for RNA and Genome Editing, Volume 711 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites, Genome oligopaint via local denaturation fluorescence in situ hybridization, Biochemical reconstitution of a type I-B CRISPR-associated transposon, Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies, and much more.
Additional sections cover Analysis of metal-dependent DNA nicking activities by Cas endonucleases, Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems, Prime editing in bacteria with BacPE, Preparation of high-purity CRISPR-based DNA base editor RNPs, Bacterial directed evolution of CRISPR base editors, Cloning and validating systems for high throughput molecular recording, Using CRISPR-Cas13 for viral nucleic acid detection, and more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xix 7. Chapter One: Measuring double-strand break repair events in mammalian cells with multi-target CRISPR - p. 1 8. Chapter Two: General and robust sample preparation strategies for cryo-EM studies of CRISPR-Cas9 and Cas12 enzymes - p. 23 9. Chapter Three: Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies - p. 41 10. Chapter Four: Biochemical reconstitution of a type I-B CRISPR-associated transposon - p. 55 11. Chapter Five: Determining the biochemical function of type IV CRISPR ribonucleoprotein complexes and accessory proteins - p. 81 12. Chapter Six: Analysis of metal-dependent DNA nicking activities by Cas endonucleases - p. 117 13. Chapter Seven: Purification and in vivo, cell-free, and in vitro characterization of CRISPR-Cas12a2 - p. 143 14. Chapter Eight: Experimental strategy for characterization of novel TnpB orthologs - p. 183 15. Chapter Nine: Hydrolytic endonucleolytic ribozyme (HYER): Systematic identification, characterization and potential application in nucleic acid manipulation - p. 197 16. Chapter Ten: Methods for Cas13a expression and purification for use in CRISPR diagnostics - p. 225 17. Chapter Eleven: Using CRISPR for viral nucleic acid detection - p. 245 18. Chapter Twelve: Preparation of high-purity RNPs of CRISPR-based DNA base editors - p. 277 19. Chapter Thirteen: Bacterial directed evolution of CRISPR base editors - p. 317 20. Chapter Fourteen: Genome editing with programmable base editors in human cells - p. 351 21. Chapter Fifteen: Prime editing in bacteria with BacPE - p. 405 22. Chapter Sixteen: A quick guide to evaluating prime editing efficiency in mammalian cells - p. 419 23. Chapter Seventeen: Using Prime Editing Guide Generator (PEGG) for high-throughput generation of prime editing sensor libraries - p. 437 24. Chapter Eighteen: Cloning and validating systems for high throughput molecular recording - p. 453 25. Chapter Nineteen: Delivery of genome editors with engineered virus-like particles - p. 475 26. Chapter Twenty: CRISPRoff epigenome editing for programmable gene silencing in human cell lines and primary T cells - p. 517
CRISPR-Cas Systems for RNA and Genome Editing, Volume 711 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites, Genome oligopaint via local denaturation fluorescence in situ hybridization, Biochemical reconstitution of a type I-B CRISPR-associated transposon, Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies, and much more.
Additional sections cover Analysis of metal-dependent DNA nicking activities by Cas endonucleases, Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems, Prime editing in bacteria with BacPE, Preparation of high-purity CRISPR-based DNA base editor RNPs, Bacterial directed evolution of CRISPR base editors, Cloning and validating systems for high throughput molecular recording, Using CRISPR-Cas13 for viral nucleic acid detection, and more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xix 7. Chapter One: Measuring double-strand break repair events in mammalian cells with multi-target CRISPR - p. 1 8. Chapter Two: General and robust sample preparation strategies for cryo-EM studies of CRISPR-Cas9 and Cas12 enzymes - p. 23 9. Chapter Three: Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies - p. 41 10. Chapter Four: Biochemical reconstitution of a type I-B CRISPR-associated transposon - p. 55 11. Chapter Five: Determining the biochemical function of type IV CRISPR ribonucleoprotein complexes and accessory proteins - p. 81 12. Chapter Six: Analysis of metal-dependent DNA nicking activities by Cas endonucleases - p. 117 13. Chapter Seven: Purification and in vivo, cell-free, and in vitro characterization of CRISPR-Cas12a2 - p. 143 14. Chapter Eight: Experimental strategy for characterization of novel TnpB orthologs - p. 183 15. Chapter Nine: Hydrolytic endonucleolytic ribozyme (HYER): Systematic identification, characterization and potential application in nucleic acid manipulation - p. 197 16. Chapter Ten: Methods for Cas13a expression and purification for use in CRISPR diagnostics - p. 225 17. Chapter Eleven: Using CRISPR for viral nucleic acid detection - p. 245 18. Chapter Twelve: Preparation of high-purity RNPs of CRISPR-based DNA base editors - p. 277 19. Chapter Thirteen: Bacterial directed evolution of CRISPR base editors - p. 317 20. Chapter Fourteen: Genome editing with programmable base editors in human cells - p. 351 21. Chapter Fifteen: Prime editing in bacteria with BacPE - p. 405 22. Chapter Sixteen: A quick guide to evaluating prime editing efficiency in mammalian cells - p. 419 23. Chapter Seventeen: Using Prime Editing Guide Generator (PEGG) for high-throughput generation of prime editing sensor libraries - p. 437 24. Chapter Eighteen: Cloning and validating systems for high throughput molecular recording - p. 453 25. Chapter Nineteen: Delivery of genome editors with engineered virus-like particles - p. 475 26. Chapter Twenty: CRISPRoff epigenome editing for programmable gene silencing in human cell lines and primary T cells - p. 517
