DNA Repair Enzymes, Part A, Volume 591 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA repair enzymes. Topics in this new release include chapters on the Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells, the Proteomic Analyses of the Eukaryotic Replication Machinery, DNA Fiber Analysis: Mind the Gap!, Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells, Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner, Base Excision Repair Variants in Cancer, and Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.
1. Cover image - p. cover 2. Title page - p. cover 3. Table of Contents - p. cover 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells - p. xviii 8. Chapter Two: Proteomic Analyses of the Eukaryotic Replication Machinery - p. 32 9. Chapter Three: DNA Fiber Analysis: Mind the Gap! - p. 53 10. Chapter Four: Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells - p. 82 11. Chapter Five: Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner - p. 95 12. Chapter Six: Base Excision Repair Variants in Cancer - p. 118 13. Chapter Seven: Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli - p. 157 14. Chapter Eight: Sequencing DNA for the Oxidatively Modified Base 8-Oxo-7,8-Dihydroguanine - p. 186 15. Chapter Nine: Xenopus laevis as Model System to Study DNA Damage Response and Replication Fork Stability - p. 210 16. Chapter Ten: Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg Extracts - p. 232 17. Chapter Eleven: Analysis of Structure-Selective Endonuclease Activities From Yeast and Human Extracts - p. 270 18. Chapter Twelve: Strategies and Methods of Transcription-Coupled Repair Studies In Vitro and In Vivo - p. 286 19. Chapter Thirteen: Reconstituted System for the Examination of Repair DNA Synthesis in Homologous Recombination - p. 306 20. Chapter Fourteen: Current and Emerging Assays for Studying the Primer Synthesis Activities of DNA Primases - p. 325 21. Chapter Fifteen: Electrical Probes of DNA-Binding Proteins - p. 353 22. Chapter Sixteen: Preparation of Stable Nitrogen Mustard DNA Interstrand Cross-Link Analogs for Biochemical and Cell Biological Studies - p. 414 23. Author Index - p. 433 24. Subject Index - p. 453
DNA Repair Enzymes, Part A, Volume 591 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA repair enzymes. Topics in this new release include chapters on the Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells, the Proteomic Analyses of the Eukaryotic Replication Machinery, DNA Fiber Analysis: Mind the Gap!, Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells, Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner, Base Excision Repair Variants in Cancer, and Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.
1. Cover image - p. cover 2. Title page - p. cover 3. Table of Contents - p. cover 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells - p. xviii 8. Chapter Two: Proteomic Analyses of the Eukaryotic Replication Machinery - p. 32 9. Chapter Three: DNA Fiber Analysis: Mind the Gap! - p. 53 10. Chapter Four: Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells - p. 82 11. Chapter Five: Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner - p. 95 12. Chapter Six: Base Excision Repair Variants in Cancer - p. 118 13. Chapter Seven: Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli - p. 157 14. Chapter Eight: Sequencing DNA for the Oxidatively Modified Base 8-Oxo-7,8-Dihydroguanine - p. 186 15. Chapter Nine: Xenopus laevis as Model System to Study DNA Damage Response and Replication Fork Stability - p. 210 16. Chapter Ten: Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg Extracts - p. 232 17. Chapter Eleven: Analysis of Structure-Selective Endonuclease Activities From Yeast and Human Extracts - p. 270 18. Chapter Twelve: Strategies and Methods of Transcription-Coupled Repair Studies In Vitro and In Vivo - p. 286 19. Chapter Thirteen: Reconstituted System for the Examination of Repair DNA Synthesis in Homologous Recombination - p. 306 20. Chapter Fourteen: Current and Emerging Assays for Studying the Primer Synthesis Activities of DNA Primases - p. 325 21. Chapter Fifteen: Electrical Probes of DNA-Binding Proteins - p. 353 22. Chapter Sixteen: Preparation of Stable Nitrogen Mustard DNA Interstrand Cross-Link Analogs for Biochemical and Cell Biological Studies - p. 414 23. Author Index - p. 433 24. Subject Index - p. 453
