DNA Repair Enzymes, Part B, Volume 592 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA Repair Enzymes. Topics in this updated volume include MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes, Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes, Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR, the Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair, and Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Chapter One: MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes - p. xx 8. Chapter Two: Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes - p. 26 9. Chapter Three: Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR - p. 47 10. Chapter Four: Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair - p. 76 11. Chapter Five: Expression and Structural Analyses of Human DNA Polymerase θ (POLQ) - p. 101 12. Chapter Six: Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein–Nucleic Acid Complexes - p. 121 13. Chapter Seven: DNA-PKcs, Allostery, and DNA Double-Strand Break Repair: Defining the Structure and Setting the Stage - p. 143 14. Chapter Eight: Single-Particle Electron Microscopy Analysis of DNA Repair Complexes - p. 157 15. Chapter Nine: Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein–DNA Complexes That Carry Out DNA Repair - p. 186 16. Chapter Ten: Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time - p. 212 17. Chapter Eleven: Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination - p. 257 18. Chapter Twelve: Detection of Reaction Intermediates in Mg2+-Dependent DNA Synthesis and RNA Degradation by Time-Resolved X-Ray Crystallography - p. 281 19. Chapter Thirteen: Analyzing the Catalytic Activities and Interactions of Eukaryotic Translesion Synthesis Polymerases - p. 327 20. Chapter Fourteen: Kinetic Methods for Studying DNA Glycosylases Functioning in Base Excision Repair - p. 356 21. Chapter Fifteen: Transient Kinetic Methods for Mechanistic Characterization of DNA Binding and Nucleotide Flipping - p. 376 22. Chapter Sixteen: What Combined Measurements From Structures and Imaging Tell Us About DNA Damage Responses - p. 415 23. Author Index - p. 457 24. Subject Index - p. 483
DNA Repair Enzymes, Part B, Volume 592 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA Repair Enzymes. Topics in this updated volume include MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes, Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes, Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR, the Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair, and Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Chapter One: MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes - p. xx 8. Chapter Two: Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes - p. 26 9. Chapter Three: Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR - p. 47 10. Chapter Four: Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair - p. 76 11. Chapter Five: Expression and Structural Analyses of Human DNA Polymerase θ (POLQ) - p. 101 12. Chapter Six: Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein–Nucleic Acid Complexes - p. 121 13. Chapter Seven: DNA-PKcs, Allostery, and DNA Double-Strand Break Repair: Defining the Structure and Setting the Stage - p. 143 14. Chapter Eight: Single-Particle Electron Microscopy Analysis of DNA Repair Complexes - p. 157 15. Chapter Nine: Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein–DNA Complexes That Carry Out DNA Repair - p. 186 16. Chapter Ten: Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time - p. 212 17. Chapter Eleven: Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination - p. 257 18. Chapter Twelve: Detection of Reaction Intermediates in Mg2+-Dependent DNA Synthesis and RNA Degradation by Time-Resolved X-Ray Crystallography - p. 281 19. Chapter Thirteen: Analyzing the Catalytic Activities and Interactions of Eukaryotic Translesion Synthesis Polymerases - p. 327 20. Chapter Fourteen: Kinetic Methods for Studying DNA Glycosylases Functioning in Base Excision Repair - p. 356 21. Chapter Fifteen: Transient Kinetic Methods for Mechanistic Characterization of DNA Binding and Nucleotide Flipping - p. 376 22. Chapter Sixteen: What Combined Measurements From Structures and Imaging Tell Us About DNA Damage Responses - p. 415 23. Author Index - p. 457 24. Subject Index - p. 483
