Droplets of Life: Membrane-Less Organelles, Biomolecular Condensates, and Biological Liquid–Liquid Phase Separation provides foundational information on the biophysics, biogenesis, structure, functions, and roles of membrane-less organelles. The study of liquid–liquid phase separation has attracted a lot of attention from disciplines such as cell biology, biophysics, biochemistry, and others trying to understand how, why, and what roles these condensates play in homeostasis and disease states in living organisms. This book's editor recruited a group of international experts to provide a current and authoritative overview of all aspects associated with this exciting area. Sections introduce membrane-less organelles (MLOs) and biomolecular condensates; MLOs in different sizes, shapes, and composition; and the formation of MLOs due to phase separation and how it can tune reactions, organize the intracellular environment, and provide a role in cellular fitness.
.1. Introduction - p. 3 2. Aqueous solution of nonionic polymers - p. 4 3. Types of two-phase systems in water - p. 19 4. Phase diagrams - p. 20 5. Properties of coexisting phases and solute partitioning in aqueous two-phase system - p. 32 6. Existing approaches to description of phase diagrams of ATPS and likely mechanism of liquid–liquid phase separation - p. 60 7. Theoretical treatments based on virial expansion model - p. 63 8. Theoretical treatment based on the excluded volume principle - p. 64 9. Chapter 2 Major structural features of membrane-less organelles - p. 83 10. Chapter 3 Biochemical and structural biology aspects of liquid–liquid phase separation: protein side of liquid–liquid phase separation, membrane-less organelles, and biomolecular condensates - p. 101 11. Chapter 4 Biochemical and structural biology aspects of liquid–liquid phase separation: an interplay between proteins and RNA - p. 133 12. Chapter 5 Liquid–liquid phase separation and biomolecular condensates in cell quiescence - p. 157 13. Chapter 6 Multiphase complex droplets - p. 173 14. Chapter 7 Techniques for the detection and analysis of LLPS and MLOs - p. 205 15. Chapter 8 Guidelines for experimental characterization of liquid–liquid phase separation in vitro - p. 233 16. Chapter 9 Liquid–liquid phase separation at the origins of life - p. 251 17. Chapter 10 Known types of membrane-less organelles and biomolecular condensates - p. 271 18. Chapter 11 Active regulation mechanisms of LLPS and MLOs biogenesis - p. 337 19. Chapter 12 Interactions and interplay of MLOs with classical membrane-bound organelles - p. 375 20. Chapter 13 Intrinsically disordered regions: a platform for regulated assembly of biomolecular condensates - p. 397 21. Chapter 14 MLOs and control of metabolic pathways - p. 431 22. Chapter 15 LLPS and regulation of transmembrane signaling - p. 447 23. Chapter 16 Phase separation in chromatin-based intranuclear processes - p. 461 24. Chapter 17 MLOstasis: liquid–liquid phase separation and biomolecular condensates in cell competition, fitness, and aging - p. 485 25. Chapter 18 Stress, membraneless organelles, and liquid–liquid phase separation - p. 505 26. Chapter 19 Roles of phase separation and condensates in autophagy - p. 531 27. Chapter 20 Liquid–liquid phase in anhydrobiosis - p. 545 28. Chapter 21 Plant biomolecular condensates - p. 557 29. Chapter 22 Droplets of life: role of phase separation in virus replication and compartmentalization - p. 567 30. Chapter 23 Liquid–liquid phase separation in neurodegenerative diseases - p. 619 31. Chapter 24 Emerging roles of liquid–liquid phase separation and membraneless organelles in cancer progression - p. 651 32. Chapter 25 Liquid–liquid phase separation, membrane-less organelles, and biomolecular condensates in cardiovascular disease - p. 663 33. Chapter 26 Phase separation and infectious diseases - p. 681 34. Index - p. 699
Droplets of Life: Membrane-Less Organelles, Biomolecular Condensates, and Biological Liquid–Liquid Phase Separation provides foundational information on the biophysics, biogenesis, structure, functions, and roles of membrane-less organelles. The study of liquid–liquid phase separation has attracted a lot of attention from disciplines such as cell biology, biophysics, biochemistry, and others trying to understand how, why, and what roles these condensates play in homeostasis and disease states in living organisms. This book's editor recruited a group of international experts to provide a current and authoritative overview of all aspects associated with this exciting area. Sections introduce membrane-less organelles (MLOs) and biomolecular condensates; MLOs in different sizes, shapes, and composition; and the formation of MLOs due to phase separation and how it can tune reactions, organize the intracellular environment, and provide a role in cellular fitness.
.1. Introduction - p. 3 2. Aqueous solution of nonionic polymers - p. 4 3. Types of two-phase systems in water - p. 19 4. Phase diagrams - p. 20 5. Properties of coexisting phases and solute partitioning in aqueous two-phase system - p. 32 6. Existing approaches to description of phase diagrams of ATPS and likely mechanism of liquid–liquid phase separation - p. 60 7. Theoretical treatments based on virial expansion model - p. 63 8. Theoretical treatment based on the excluded volume principle - p. 64 9. Chapter 2 Major structural features of membrane-less organelles - p. 83 10. Chapter 3 Biochemical and structural biology aspects of liquid–liquid phase separation: protein side of liquid–liquid phase separation, membrane-less organelles, and biomolecular condensates - p. 101 11. Chapter 4 Biochemical and structural biology aspects of liquid–liquid phase separation: an interplay between proteins and RNA - p. 133 12. Chapter 5 Liquid–liquid phase separation and biomolecular condensates in cell quiescence - p. 157 13. Chapter 6 Multiphase complex droplets - p. 173 14. Chapter 7 Techniques for the detection and analysis of LLPS and MLOs - p. 205 15. Chapter 8 Guidelines for experimental characterization of liquid–liquid phase separation in vitro - p. 233 16. Chapter 9 Liquid–liquid phase separation at the origins of life - p. 251 17. Chapter 10 Known types of membrane-less organelles and biomolecular condensates - p. 271 18. Chapter 11 Active regulation mechanisms of LLPS and MLOs biogenesis - p. 337 19. Chapter 12 Interactions and interplay of MLOs with classical membrane-bound organelles - p. 375 20. Chapter 13 Intrinsically disordered regions: a platform for regulated assembly of biomolecular condensates - p. 397 21. Chapter 14 MLOs and control of metabolic pathways - p. 431 22. Chapter 15 LLPS and regulation of transmembrane signaling - p. 447 23. Chapter 16 Phase separation in chromatin-based intranuclear processes - p. 461 24. Chapter 17 MLOstasis: liquid–liquid phase separation and biomolecular condensates in cell competition, fitness, and aging - p. 485 25. Chapter 18 Stress, membraneless organelles, and liquid–liquid phase separation - p. 505 26. Chapter 19 Roles of phase separation and condensates in autophagy - p. 531 27. Chapter 20 Liquid–liquid phase in anhydrobiosis - p. 545 28. Chapter 21 Plant biomolecular condensates - p. 557 29. Chapter 22 Droplets of life: role of phase separation in virus replication and compartmentalization - p. 567 30. Chapter 23 Liquid–liquid phase separation in neurodegenerative diseases - p. 619 31. Chapter 24 Emerging roles of liquid–liquid phase separation and membraneless organelles in cancer progression - p. 651 32. Chapter 25 Liquid–liquid phase separation, membrane-less organelles, and biomolecular condensates in cardiovascular disease - p. 663 33. Chapter 26 Phase separation and infectious diseases - p. 681 34. Index - p. 699
