Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge.
1. Introduction to the Second Edition - p. xv 2. General Introduction - p. xvii 3. Part I: Historical Perspectives and Classical Methods - p. xxi 4. Chapter 1. Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells - p. 3 5. Chapter 2. Primary Cell Cultures of Drosophila Embryonic Cells - p. 47 6. Chapter 3. Continuous Drosophila Cell Lines Established In Vitro - p. 61 7. Chapter 4. Cells or Tissues in Course of Differentiation - p. 81 8. Chapter 5. Hormonal Control of Development of Insects: Implications in Organ or Cell Cultures of Drosophila - p. 103 9. Part II: Contemporary Approaches - p. 125 10. Chapter 6. Directed Genetic Methods for Establishing Continuous Cell Lines - p. 135 11. Chapter 7. Organ Culture Methods for the Drosophila Wing Imaginal Disc - p. 145 12. Chapter 8. Functional Genomics Screens in Drosophila Cells - p. 165 13. Chapter 9. CRISPR-Based Perturbation of Gene Function in Drosophila Cells - p. 193 14. Chapter 10. Compendium of Drosophila Cell Line Resources and Plasmid Vectors at the Drosophila Genome Resource Center - p. 207 15. Index - p. 227
Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge.
1. Introduction to the Second Edition - p. xv 2. General Introduction - p. xvii 3. Part I: Historical Perspectives and Classical Methods - p. xxi 4. Chapter 1. Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells - p. 3 5. Chapter 2. Primary Cell Cultures of Drosophila Embryonic Cells - p. 47 6. Chapter 3. Continuous Drosophila Cell Lines Established In Vitro - p. 61 7. Chapter 4. Cells or Tissues in Course of Differentiation - p. 81 8. Chapter 5. Hormonal Control of Development of Insects: Implications in Organ or Cell Cultures of Drosophila - p. 103 9. Part II: Contemporary Approaches - p. 125 10. Chapter 6. Directed Genetic Methods for Establishing Continuous Cell Lines - p. 135 11. Chapter 7. Organ Culture Methods for the Drosophila Wing Imaginal Disc - p. 145 12. Chapter 8. Functional Genomics Screens in Drosophila Cells - p. 165 13. Chapter 9. CRISPR-Based Perturbation of Gene Function in Drosophila Cells - p. 193 14. Chapter 10. Compendium of Drosophila Cell Line Resources and Plasmid Vectors at the Drosophila Genome Resource Center - p. 207 15. Index - p. 227
